- Research article
- Open Access
Insulin resistance and its association with the components of the metabolic syndrome among obese children and adolescents
BMC Public Health volume 10, Article number: 318 (2010)
Insulin resistance is the primary metabolic disorder associated with obesity; yet little is known about its role as a determinant of the metabolic syndrome in obese children. The aim of this study is to assess the association between the degree of insulin resistance and the different components of the metabolic syndrome among obese children and adolescents.
An analytical, cross-sectional and population-based study was performed in forty-four public primary schools in Campeche City, Mexico. A total of 466 obese children and adolescents between 11-13 years of age were recruited. Fasting glucose and insulin concentrations, high density lipoprotein cholesterol, triglycerides, waist circumference, systolic and diastolic blood pressures were measured; insulin resistance and metabolic syndrome were also evaluated.
Out of the total population studied, 69% presented low values of high density lipoprotein cholesterol, 49% suffered from abdominal obesity, 29% had hypertriglyceridemia, 8% presented high systolic and 13% high diastolic blood pressure, 4% showed impaired fasting glucose, 51% presented insulin resistance and 20% metabolic syndrome. In spite of being obese, 13% of the investigated population did not present any metabolic disorder. For each one of the components of the metabolic syndrome, when insulin resistance increased so did odds ratios as cardiometabolic risk factors.
Regardless of age and gender an increased degree of insulin resistance is associated with a higher prevalence of disorders in each of the components of the metabolic syndrome and with a heightened risk of suffering metabolic syndrome among obese children and adolescents.
The World Health Organization recognizes overweight and obesity in children and adolescents as worldwide public health problems. Mexico is one of the countries which suffer most from these.
Indeed, according to Mexican National Health and Nutrition Surveys, in 1999  the combined prevalence of overweight and obesity in school-age children was 18.6%; by 2006, such prevalence had increased to 26%, which represents an average increase of 1.1 percentage points per year .
Insulin resistance (IR) is the primary metabolic disorder associated with obesity and is defined as a diminished ability of insulin to stimulate glucose uptake by skeletal muscle and adipose tissue, in addition to reducing insulin's ability to suppress hepatic glucose production and output . Some of the several disorders associated with IR that have been described include systemic inflammation, increases in fibrinolysis, endothelial dysfunction, and atherosclerosis , all of which can first appear during childhood in obese individuals . Although the hyperinsulinemic-euglycemic clamp is considered the gold standard for evaluating and measuring IR, the technical difficulties associated with this method have led to the development of less invasive methods. Among these, the homeostasis model assessment of insulin resistance index (HOMA-IR) is one of the most commonly used . It is worth noting that no consensus exists concerning the HOMA-IR cut-off points that define IR among the pediatric population, although there is general agreement that IR is a common pathway for the development of glucose metabolism disorders, dyslipidemias, and high blood pressure, all of which are components of the metabolic syndrome (MS) [7–9]. In turn, MS is a risk factor for the subsequent development of type-2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) .
Among children, MS is not well characterized, and there is a lack of agreement as to its components and cut-off points, though most authors include among the former abdominal obesity, high blood pressure, glucose abnormalities, and dyslipidemias [11, 12]. Due to this lack of consensus, the reported prevalence of MS among children and adolescents shows a great deal of variability [13, 14]. For instance, in studies including children and adolescents with varying nutritional conditions, the prevalence lies between 2.5% and 12.9% . However, when only overweight and obese children and adolescents are included, the prevalence increases and falls within the range 26% -31.2% [13–15].
In children and adolescents, a direct relationship between the degree of obesity and the prevalence of MS has been reported [16, 17]; however, it is not exactly known how and to what extent IR is associated with each of the components of MS. In this context, this study aims to elucidate the prevalence of IR and to evaluate the association of IR with each of the components of MS among obese children and adolescents.
The study was conducted in all of the forty-four schools that compose the public school system in Campeche City, Mexico.
Prior to the study, ethical clearance was obtained from Campeche State research ethics committees and school authorities. The study consisted of two stages, the first of which aimed to identify children suffering from obesity. For this purpose, weight and height were measured by four nurses previously trained to follow international anthropometric guidelines. The study included 4,937 children between 11 and 13 years of age attending the 5th and 6th grades. Obesity was defined as a body mass index (BMI) percentile ≥95th for the child's age and gender according to the Centers for Disease Control (CDC) 2000 references . Following this criterion, 1,475 children were classified as obese. Out of this total, written parental and child informed consent was obtained from 600 randomly selected children. From this group, complete blood samples were collected from 466 children.
During the second stage, blood pressure (BP) was obtained using the auscultatory method, and waist circumference (WC) was measured with children in the standing position, placing the metric strip at the midpoint between the lower rib and the iliac crest after a normal exhalation. In addition, a sample of venous blood was obtained after a 12-hour fast in order to determine concentrations of insulin by chemiluminescence immunoassay (Access Beckman Coulter Instruments, Brea California), glucose, and plasma lipids by an enzymatic method, and high density lipoprotein cholesterol (HDL-C) assayed by the addition of magnesium ions (Synchron CX® Beckman Instruments, Brea California). In all cases, commercial enzymatic kits were employed. Low density lipoprotein cholesterol (LDL-C) levels were estimated using the Friedewald formula, as modified by De Long .
Definition of MS and its components
MS was defined according to guidelines by the International Diabetes Federation (IDF) , the only exception being that the BP criteria were used according to the North American Task Force guidelines . For each of the components of MS, the following cut-off points were used: Hypertriglyceridemia (triglycerides ≥ 150 mg/dL), low HDL-C (HDL-C ≤ 40 mg/dL), high blood pressure (systolic and/or diastolic BP ≥ 90th percentile for children's age, gender, and height), impaired fasting glucose (fasting glucose ≥ 100 mg/dL), and abdominal obesity (WC ≥ percentile 90th for children's age, gender, and ethnic origin) . MS was diagnosed when three or more of the previously described components were present. Hypercholesterolemia (total cholesterol ≥ 200 mg/dL) and high values of LDL-C (LDL-C ≥ 130 mg/dL) were defined according by the American Academy of Pediatrics .
Definition of IR
IR was determined through HOMA-IR, which was calculated using the following equation: [(fasting glucose (mg/dL))(fasting insulin (μU/mL))/405)] . A HOMA-IR value of 3.4 was chosen as the cut-off point to define IR as it has been suggested that beyond this value, which corresponds to the 90th percentile of a population of healthy children, IR becomes a cardiovascular risk factor .
Means and standard deviations and prevalence of anthropometric and metabolic variables were obtained. These measures were compared by gender using Student's t test or X2 test. Four categories were derived for HOMA-IR percentiles: <25th, 25-49.9th, 50-74.9th, and ≥75th. In order to assess the risk of presenting disorders in each of the MS components, measures of these were compared according to the aforementioned HOMA-IR percentile categories through logistic regression analysis. Statistically significant differences were assumed if the P-value was < 0.05.
Data were processed with STATA, SE v.9.0, and EPIINFO 3.3.2 according to the CDC 2000 reference .
Table 1 depicts the anthropometric features, BP and metabolic profile of the obese children and adolescents who participated in the study. For both sexes, the mean age was 11.3, SD 0.8 years; the mean BMI, 27.3 kg/m², SD 3.0, which corresponds to the 97.5th percentile for age and gender; and the mean WC, 85.0 cm, SD 7.8.
No difference was observed between sexes in the values of systolic and diastolic BP. However, in both sexes systolic BP fell into the 49.5th percentile, whereas diastolic BP fell into the 60.9th percentile, according to the reference values for gender, age, and height  (these data are not shown in the tables).
The same table summarizes the values of glucose, insulin, and lipids in the investigated children and adolescent population. Girls exhibited a higher concentration of fasting insulin and higher HOMA-IR values compared to boys, but smaller concentrations of fasting glucose, total cholesterol, and LDL-C.
Table 2 depicts the prevalence of cardiometabolic risk factors and the components of MS among the study participants. The prevalence of the components of MS in this population was as follows: abdominal obesity, 49%; high systolic BP, 8%; high diastolic BP, 13%; impaired fasting glucose, 4%; hypertriglyceridemia, 29%; and low HDL-C, 69%. Although 56% of the population suffered from hyperinsulinemia, this condition was observed in 71% of girls but only in 45% of boys (P < 0.001); likewise, IR was more common among girls than boys, 63% versus 43%, respectively (P < 0.001). The prevalence of heightened total cholesterol levels was higher among boys (14%) than girls (7%) (P = 0.013), a similar pattern also found in relation to increased LDL-C values, where the prevalence was 15% and 6% (P = 0.004) among boys and girls, respectively. Although 13% of these children and adolescents did not have any components associated with MS, 20% had three or more of such components.
Using the group with HOMA-IR values below the 25th percentile as a reference, table 3 shows how, for each one of the components of MS, when IR increases so does the "odds ratio (OR)" as a cardiometabolic risk factor. WC begins to be significant from the 25th percentile of IR (P = 0.014). From the 50th percentile onwards, in addition to WC, also diastolic BP, glucose and triglycerides become significant (P < 0.05). Above the 75th percentile, all of the OR are significant (P < 0.05), except systolic and diastolic BP.
Likewise, Table 4 shows the ORs of suffering MS according to IR categories. From this table, it is apparent that the Odds of developing MS (adjusted for gender and age) increases as a function of IR. Such OR is 5.5 (95% CI 2.6-11.6) times greater when IR is above the 75th percentile.
Our results show that higher levels of IR are associated with a greater degree of alterations in the components of the MS in the population studied, half of which presented IR.
The apparently normal fasting glucose levels in this population are maintained by a compensatory mechanism based on hyperinsulinism, which is reflected in HOMA-IR values . However, when impaired fasting glucose prevalence is analyzed through ORs, it is possible to observe that increased levels of IR are associated with rising OR.
Girls presented the highest insulin levels and HOMA-IR values, along with lower glucose concentrations. This pattern could be a consequence of the fact that, at equal ages, girls can enter puberty up to two years earlier than boys [26, 27] - therefore, more girls would have reached higher pubertal stages. However - and this is a limitation of this study - no information was collected about either pubertal stage or growth and sexual hormones, factors which could influence the prevalence of the rise in IR .
In accordance with previous studies on adolescents and adults, the IR reported in this study is associated with the primary alterations in the lipid profile; hyperinsulinism increases the free fatty acid release and the triglyceride synthesis, which results in hypertriglyceridemia [29, 30]. Likewise increased hepatic lipase activity can account for the rise in high-density lipoprotein depuration, producing hypoalphalipoproteinemia . However, the high prevalence of low C-HDL levels observed in this study is one of the highest reported in the literature [13, 30, 32]. Regardless of its cause, it is a risk factor for the development of cardiovascular events during adulthood. Such events stem from both genetic and environmental factors. Indeed, it has been reported that individuals of Turkish descent display greater hepatic lipase activity, which augments the depuration of this lipoprotein and is associated with lower levels of HDL-C . However, it is not known whether population of Mexican descent could have a polymorphism such as that identified among their Turkish counterparts that may explain the higher prevalence of low HDL-C observed in this study. What is known is that, among individuals of Mexican descent, an association has been described between a variant of gene ABCA1 and lower HDL-C levels, along with a greater risk of developing obesity, MS, and early onset T2DM . As far as environmental factors are concerned, lower levels of this lipoprotein can be explained in terms of changing eating habits . The diet of Mexican children resembles ever more that of their North American counterparts , a diet rich in simple sugars and animal fats, but with limited amounts of fiber .
Although the association between IR and OR of suffering high blood pressure was not statistically significant, the observed trend in this population can be associated with the different disorders stemming from obesity  among which IR stands out . Hyperinsulinism increases renal sodium absorption and sympathetic tone , which combines with altered vasodilatation, which in turn is a secondary effect of nitric oxide deficient secretion by the vascular endothelium . Therefore, it is to be expected that if such IR-induced hyperinsulinism continues, permanent alterations such as atherosclerosis and hypertension will eventually develop.
Furthermore, the prevalence of MS reported in this study - around 20% - might stem from the employed definition, which relied on stricter cut-off points than those used in other studies . In studies where Cook's criteria was applied, the prevalence of MS among North American obese adolescents was 27% , in others following the III Adult Treatment Panel criteria, the prevalence was 26.1% .
In order to ascertain the real prevalence of this syndrome among children, agreement is needed concerning the cut-off points for each of the syndrome's components; likewise, categories for pubertal stage and gender need to be established . Notwithstanding the heterogeneity of current definitions, the notion of MS has been useful for identifying individual children at risk of developing CVD and T2DM .
As mentioned above, 87% of the study participants presented at least some kind of functional or metabolic disorder. Nevertheless, in spite of being obese, almost 13% of the study population did not present any kind of disorder, a phenotype known as "metabolically healthy but obese individuals" . Among adults, a 9.7% prevalence of this phenotype has been reported in the literature; interestingly, within this group no family history of T2DM has been found . This suggests that protection against cardiometabolic disorders, even in the presence of obesity, can have genetic features .
A further limitation of this study derives from the fact that cross-sectional studies are not able to establish causality, only associations between different variables.
However, given the close relationship between obesity, IR, and MS - with its attendant risk of developing co-morbidities, such as T2DM and CVD - it is imperative to implement prevention, diagnosis, and early treatment measures for obesity involving all sectors of society.
This study has confirmed that among obese children and adolescents, regardless of age and gender, an increased degree of insulin resistance is associated with a higher prevalence of disorders in each of the components of the metabolic syndrome and with a heightened risk of suffering MS.
HOMA-IR values above 3.4, which correspond to the 50th percentile of this population, were associated with an increased risk of having MS, compared to the lowest percentile of HOMA-IR values.
Rivera Dommarco J, Shamah-Levy T, Villalpando Hernández S, González de Cossio T, Hernández Prado B, Sepúlveda J: Encuesta Nacional de Nutrición 1999. Estado nutricio de niños y mujeres en México. 2001, Cuernavaca Morelos México: Instituto Nacional de Salud Pública
Olaiz-Fernández G, Rivera-Dommarco J, Shamah-Levy T, Rojas R, Villalpando-Hernández S, Hernández-Avila M, Sepúlveda-Amor J: Encuesta nacional de salud y nutrición 2006. 2006, Cuernavaca Morelos México: Instituto Nacional de Salud Pública
Chiarelli F, Marcovecchio ML: Insulin resistance and obesity in childhood. Eur J Endocrinol. 2008, 159 (Suppl 1): S67-S74. 10.1530/EJE-08-0245.
Dandona P, Aljada A, Mohanty P: The anti-inflammatory and potential anti-atherogenic effect of insulin: a new paradigm. Diabetologia. 2002, 45 (6): 924-930. 10.1007/s00125-001-0766-5.
Peña AS, Wiltshire E, MacKenzie K, Gent R, Piotto L, Hirte C, Couper J: Vascular endothelial relates to body mass nonobese children and smooth muscle function index and glucose in obese and nonobese children. J Clin Endocrinol Metab. 2006, 91 (11): 4467-4471. 10.1210/jc.2006-0863.
Radziuk J: Insulin sensitivity and its measurement: structural commonalities among the methods. J Clin Endocrinol Metab. 2000, 85 (12): 4426-4433. 10.1210/jc.85.12.4426.
Flores-Huerta S, Klünder-Klünder M, Reyes-de-la-Cruz L, Santos JI: Increase in body mass index and waist circumference is associated with high blood pressure in children and adolescents in Mexico City. Arch Med Res. 2009, 40 (3): 208-215. 10.1016/j.arcmed.2009.02.009.
Perichart-Perera O, Balas-Nakash M, Schiffman-Selechnik E, Barbato-Dosal A, Vadillo-Ortega F: Obesity increases metabolic syndrome risk factors in school-aged children from an urban school in Mexico City. J Am Diet Assoc. 2007, 107 (1): 81-91. 10.1016/j.jada.2006.10.011.
Taksali SE, Caprio S, Dziura J, Dufour S, Calí AMG, Goodman R, Papademetris X, Burgert TS, Pierpont BM, Savoye M, et al: High visceral and low abdominal subcutaneous fat stores in the obese adolescent: a determinant of an adverse metabolic phenotype. Diabetes. 2008, 57: 367-371. 10.2337/db07-0932.
Burke V, Beilin LJ, Simmer K, Oddy WH, Blake KV, Doherty D, Kendall GE, Newnham JP, Landau LI, Stanley FJ: Predictors of body mass index and associations with cardiovascular risk factors in Australian children: a prospective cohort study. Int J Obes. 2005, 29 (1): 15-23. 10.1038/sj.ijo.0802750.
Amemiya S, Dobashi K, Urakami T, Sugihara S, Ohzeki T, Tajima N: Metabolic syndrome in youths. Pediatr Diabetes. 2007, 8 (Suppl 9): 48-54. 10.1111/j.1399-5448.2007.00332.x.
Zimmet P, Alberti G, Kaufman F, Tajima N, Silink M, Arslanian S, Wong G, Bennett P, Shaw J, Caprio S: The metabolic syndrome in children and adolescents. Lancet. 2007, 369 (9579): 2059-2061. 10.1016/S0140-6736(07)60958-1.
de Ferranti SD, Gauvreau K, Ludwig DS, Neufeld EJ, Newburger JW, Rifai N: Prevalence of the metabolic syndrome in American adolescents. Findings from the third national health and nutrition examination survey. Circulation. 2004, 110: 2494-2497. 10.1161/01.CIR.0000145117.40114.C7.
Cruz ML, Goran MI: The metabolic syndrome in children and adolescents. Current Diabetes Reports. 2004, 4: 53-62. 10.1007/s11892-004-0012-x.
Rodriguez-Moran M, Salazar-Vazquez B, Violante R, Guerrero-Romero F: Metabolic syndrome among children and adolescents aged 10-18 years. Diabetes Care. 2004, 27 (10): 2516-2517. 10.2337/diacare.27.10.2516.
Cook S, Weitzman M, Auinger P, Nguyen M, Dietz WH: Prevalence of a metabolic syndrome phenotype in adolescents: findings from the third National Health and Nutrition Examination Survey, 1988-1994. Arch Pediatr Adolesc Med. 2003, 157 (8): 821-827. 10.1001/archpedi.157.8.821.
Messiah SE, Arheart KL, Luke B, Lipshultz SE, Miller TL: Relationship between body mass index and metabolic syndrome risk factors among US 8- to 14-year-olds, 1999 to 2002. J Pediatr. 2008, 153 (2): 215-221. 10.1016/j.jpeds.2008.03.002.
Kuczmarski RJ, Ogden CL, Guo SS, Grummer-Strawn LM, Flegal KM, Mei Z, Wei R, Curtin LR, Roche AF, Johnson CL: 2000 CDC growth charts for the United States: Methods and development. National Center for Health Statistics. Vital Health Stat. 2002, 11 (246):
DeLong DM, DeLong ER, Wood PD, Lippel K, BM R: A comparison of methods for the estimation of plasma low- and very low-density lipoprotein cholesterol. The Lipid Research Clinics Prevalence Study. JAMA. 1986, 256 (17): 2372-2377. 10.1001/jama.256.17.2372.
National high blood pressure education program working group on high blood pressure in children and adolescents: The fourth report on the diagnosis, evaluation, and treatment of high blood pressure in children and adolescents. Pediatrics. 2004, 114 (Suppl 2): 555-576. 10.1542/peds.114.2.S2.555.
Fernández JR, Redden DT, Pietrobelli A, Allison DL: Waist circumference percentiles in nationally representative samples of African-American, European-American, and Mexican-American children and adolescents. J Pediatr. 2004, 145 (4): 439-444. 10.1016/j.jpeds.2004.06.044.
Daniels SR, Greer FR, Committee on Nutrition: Lipid screening and cardiovascular health in childhood. Pediatrics. 2008, 122 (1): 198-208. 10.1542/peds.2008-1349.
Keskin M, Kurtoglu S, Kendirci M, Atabek ME, Yazici C: Homeostasis model assessment is more reliable than the fasting glucose/insulin ratio and quantitative insulin sensitivity check index for assessing insulin resistance among obese children and adolescents. Pediatrics. 2005, 115 (4): e500-503. 10.1542/peds.2004-1921.
García-Cuartero B, García-Lacalle C, Jiménez-Lobo C, González-Vergaz A, Calvo-Rey C, Alcázar-Villar MJ, Díaz-Martínez E: Índice HOMA y QUICKI, insulina y péptido C en niños sanos. Puntos de corte de riesgo cardiovascular. An Pediatr (Barc). 2007, 66 (5): 481-490. 10.1157/13102513.
Lebovitz HE, Banerji MA: Point: Visceral adiposity is causally related to insulin resistance. Diabetes Care. 2005, 28 (9): 2322-2325. 10.2337/diacare.28.9.2322.
Hirschler V, Maccallini G, Karam C, González C, Aranda C: Are girls more insulin-resistant than boys?. Clin Biochem. 2009, 42 (10-11): 1051-1056. 10.1016/j.clinbiochem.2009.03.002.
Kaplowitz PB, Slora EJ, Wasserman RC, Pedlow SE, Herman-Giddens ME: Earlier onset of puberty in girls: Relation to increased body mass index and race. Pediatrics. 2001, 108 (2): 347-353. 10.1542/peds.108.2.347.
Brufani C, Tozzi A, Fintini D, Ciampalini P, Grossi A, Fiori R, Kiepe D, Manco M, Schiaffini R, Porzio O, et al: Sexual dimorphism of body composition and insulin sensitivity across pubertal development in obese Caucasian subjects. Eur J Endocrinol. 2009, 160 (5): 769-775. 10.1530/EJE-08-0878.
Aguilar-Salinas CA, Olaiz G, Valles V, Torres JMR, Perez FJG, Rull JA, Rojas R, Franco A, Sepulveda J: High prevalence of low HDL cholesterol concentrations and mixed hyperlipidemia in a Mexican nationwide survey. J Lipid Res. 2001, 42 (8): 1298-1307.
Posadas-Sánchez R, Posadas-Romero C, Zamora-González C, Mendoza-Pérez E, Cardoso-Saldaña G, Yamamoto-Kimura L: Lipid and lipoprotein profiles and prevalence of dyslipidemia in Mexican adolescents. Metabolism: clinical and experimental. 2007, 56 (12): 1666-1672.
Decsi T, Molnar D: Insulin resistance syndrome in children. Pediatr Drugs. 2003, 5 (5): 291-299.
Jago R, Harrell JS, McMurray RG, Edelstein S, Ghormli LE, Bassin S: Prevalence of abnormal lipid and blood pressure values among an ethnically diverse population of eighth-grade adolescents and screening implications. Pediatrics. 2006, 117: 2065-2073. 10.1542/peds.2005-1716.
Mahley RW, Pepin J, Palaoglu KE, Malloy MJ, Kane JP, Bersot TP: Low levels of high density lipoproteins in Turks, a population with elevated hepatic lipase: high density lipoprotein characterization and gender-specific effects of apolipoprotein E genotype. J Lipid Res. 2000, 41 (8): 1290-1301.
Villarreal-Molina MT, Aguilar-Salinas CA, Rodríguez-Cruz M, Riaño D, Villalobos-Comparan M, Coral-Vazquez R, Menjivar M, Yescas-Gomez P, Königsoerg-Fainstein M, Romero-Hidalgo S, et al: The ATP-Binding cassette transporter A1 R230C variant affects HDL cholesterol levels and BMI in the Mexican population. Diabetes. 2007, 56 (7): 1881-1887. 10.2337/db06-0905.
Lichtenstein AH: Thematic review series: Patient-Oriented Research. Dietary fat, carbohydrate, and protein: effects on plasma lipoprotein patterns. J Lipid Res. 2006, 47 (8): 1661-1667. 10.1194/jlr.R600019-JLR200.
Dixon LB, Sundquist J, Winkleby M: Differences in Energy, Nutrient, and Food Intakes in a US Sample of Mexican-American Women and Men: Findings from the Third National Health and Nutrition Examination Survey, 1988-1994. Am J Epidemiol. 2000, 152 (6): 548-557. 10.1093/aje/152.6.548.
Flores-Huerta S, Acosta-Cázares B, Rendón-Macías ME, Klünder-Klünder M, Gutiérrez-Trujillo G: ENCOPREVENIMSS 2003, 2004 y 2005. 5. Consumo de alimentos saludables, o con riesgo para la salud, 2004. Rev Med Inst Mex Seguro Soc. 2006, 44 (Suppl 1): S63-S78.
Sinaiko AR, Steinberger J, Moran A, Hong C-P, Prineas RJ, Jacobs DR: Influence of insulin resistance and body mass index at age 13 on systolic blood pressure, triglycerides, and high-density lipoprotein cholesterol at age 19. Hypertension. 2006, 48 (4): 730-736. 10.1161/01.HYP.0000237863.24000.50.
Reaven GM, Lithell H, Landsberg L: Hypertension and associated metabolic abnormalities: The role of insulin resistance and the sympathoadrenal system. N Engl J Med. 1996, 334 (6): 374-382. 10.1056/NEJM199602083340607.
Chen W, Srinivasan SR, Elkasabany A, Ellsworth DL, Boerwinkle E, Berenson GS: Combined effects of endothelial nitric oxide synthase gene polymorphism (G894T) and insulin resistance status on blood pressure and familial risk of hypertension in young adults: the Bogalusa Heart Study. Am J Hipertens. 2001, 14 (10): 1046-1052. 10.1016/S0895-7061(01)02192-6.
Jolliffe CJ, Janssen I: Development of age-specific adolescent metabolic syndrome criteria that are linked to the Adult Treatment Panel III and International Diabetes Federation Criteria. J Am Coll Cardiol. 2007, 49 (8): 891-898. 10.1016/j.jacc.2006.08.065.
Perez-Gomez G, Huffman FG: Risk factors for type 2 diabetes and cardiovascular diseases in Hispanic adolescents. J Adolesc Health. 2008, 43 (5): 444-450. 10.1016/j.jadohealth.2008.03.010.
Marini MA, Succurro E, Frontoni S, Hribal ML, Andreozzi F, Lauro R, Perticone F, Sesti G: Metabolically healthy but obese women have an intermediate cardiovascular risk profile between healthy nonobese women and obese insulin-resistant women. Diabetes Care. 2007, 30 (8): 2145-2147. 10.2337/dc07-0419.
Dvorak RV, DeNino WF, Ades PA, Poehlman ET: Phenotypic characteristics associated with insulin resistance in metabolically obese but normal-weight young women. Diabetes. 1999, 48 (11): 2210-2214. 10.2337/diabetes.48.11.2210.
Bouhours-Nouet N, Dufresne S, de Casson FB, Mathieu E, Douay O, Gatelais F, Rouleau S, Coutant R: High birth weight and early postnatal weight gain protect obese children and adolescents from truncal adiposity and insulin resistance. Diabetes Care. 2008, 31 (5): 1031-1036. 10.2337/dc07-1647.
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The authors declare that they have no competing interests.
CJL conceived of the study, and participated in its design and coordination. MKK and PMB performed the statistical analysis and revised the manuscript critically. AMA participated in administrative and technical support, obtained data and supervised the work field. EMD provided administrative and technical support, obtained data and analysed blood samples. SFH participated in the conception and design of the research question as well as in fieldwork supervision. All authors were involved in drafting the manuscript. All of them read and approved the final version of the manuscript.
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Juárez-López, C., Klünder-Klünder, M., Medina-Bravo, P. et al. Insulin resistance and its association with the components of the metabolic syndrome among obese children and adolescents. BMC Public Health 10, 318 (2010). https://doi.org/10.1186/1471-2458-10-318
- Insulin Resistance
- Metabolic Syndrome
- Waist Circumference
- Obese Child
- High Density Lipoprotein Cholesterol