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SARS-CoV-2 antibody prevalence among homeless people and shelter workers in Denmark: a nationwide cross-sectional study

Abstract

Background

People experiencing homelessness (PEH) and associated shelter workers may be at higher risk of infection with “Severe acute respiratory syndrome coronavirus 2” (SARS-CoV-2). The aim of this study was to determine the prevalence of SARS-CoV-2 among PEH and shelter workers in Denmark.

Design and methods

In November 2020, we conducted a nationwide cross-sectional seroprevalence study among PEH and shelter workers at 21 recruitment sites in Denmark. The assessment included a point-of-care test for antibodies against SARS-CoV-2, followed by a questionnaire. The seroprevalence was compared to that of geographically matched blood donors considered as a proxy for the background population, tested using a total Ig ELISA assay.

Results

We included 827 participants in the study, of whom 819 provided their SARS-CoV-2 antibody results. Of those, 628 were PEH (median age 50.8 (IQR 40.9–59.1) years, 35.5% female) and 191 were shelter workers (median age 46.6 (IQR 36.1–55.0) years and 74.5% female). The overall seroprevalence was 6.7% and was similar among PEH and shelter workers (6.8% vs 6.3%, p = 0.87); and 12.2% among all participants who engaged in sex work. The overall participant seroprevalence was significantly higher than that of the background population (2.9%, p < 0.001). When combining all participants who reported sex work or were recruited at designated safe havens, we found a significantly increased risk of seropositivity compared to other participants (OR 2.23, 95%CI 1.06–4.43, p = 0.02). Seropositive and seronegative participants reported a similar presence of at least one SARS-CoV-2 associated symptom (49% and 54%, respectively).

Interpretations

The prevalence of SARS-CoV-2 antibodies was more than twice as high among PEH and associated shelter workers, compared to the background population. These results could be taken into consideration when deciding in which phase PEH are eligible for a vaccine, as part of the Danish national SARS-CoV-2 vaccination program rollout.

Funding

TrygFonden and HelseFonden.

Peer Review reports

Summary of key points

Homeless and shelter workers had a SARS-CoV-2 seroprevalence of 6.7%. Those who reported sex work were at a fourfold elevated risk of being SARS-CoV-2 seropositive. There was no significant association between reported symptoms and seropositivity, nor between substance abuse and seropositivity. 

Introduction

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has since its emergence in China in 2019 caused a global pandemic. As of April 26th 2021 an estimated 146 million people worldwide were infected and more than 3 million people has died from SARS-CoV-2 [1]. The first confirmed case of SARS-CoV-2 in Denmark was detected on February 27th, 2020. Since then, there have been more than 246 460 confirmed cases of SARS-CoV-2 in Denmark [2]. More than 12 880 of the Danish SARS-CoV-2 patients were hospitalized between January 2020 and March 2021.

Vulnerable groups including people experiencing homelessness (PEH) have challenges in accessing health care systems and public health information [3], including that of SARS-CoV-2. A French study found the overall seroprevalence among PEH to be 52.1% which was 4.3 times higher than the modelled estimate for the general population in Ile de France (12%) [4]. Limited knowledge of protection against SARS-CoV-2 among vulnerable individuals such as PEH is likely to increase the risk of infection for both PEH and people in their proximity, such as shelter workers. Additionally, the recommended guidelines to prevent the spread of SARS-CoV-2 might not be feasible due to inadequate access to handwash, protective equipment and difficulties in practicing social distancing [5, 6]. An estimated 6,431 (0.1%) Danes are categorized as homeless, of whom 2.666 (41.5%) are registered in the Capital Region of Denmark and the rest are distributed throughout the largest cities in Denmark [7, 8]. PEH have more physical and mental health issues than the background population [9, 10] and often engage in substance abuse [7], which could increase their risk of infection and of a serious course of disease by SARS-CoV-2 [11, 12]. Furthermore, a fear of experiencing serious withdrawal symptoms may prevent PEH from both testing and subsequent self-isolation. Furthermore, crowded living conditions in shelters and public spaces where PEH reside, constitute a potential risk of becoming epicenters, as congregate settings have proven to be associated to high exposure to SARS-CoV-2 [4].

Some PEH engage in sex work which may further increase the risk of infection with SARS-CoV-2, due to direct physical contact with clients [13]. In addition, sex workers are known to have a high prevalence of HIV [14] and other underlying health conditions [15, 16], which may add to their risk of SARS-CoV-2 progressing to severe illness [12].

Systematic screening for SARS-CoV-2 antibodies is an important tool in the surveillance of the current pandemic. Information on the prevalence of SARS-CoV-2 infection among vulnerable groups such as PEH is important to assess the need for preventative measures in such groups, to provide information about support estimations of the overall prevalence of SARS-CoV-2 infection and to help guide the public health response in the future. This study is part of the national surveillance study “Testing Denmark”, aimed at assessing the extent and impact of SARS-CoV-2 infection in Denmark [17]. The aim of the present study was to determine SARS-CoV-2 seropositivity among PEH and shelter workers in Denmark, and to study risk factors for infection and clinical presentation in PEH.

Design and methods

Study design and sampling strategy

Between November 2nd and 20th 2020 we conducted a nationwide, cross-sectional seroprevalence study to determine the prevalence of SARS-CoV-2 antibodies among PEH. In addition, we also included shelter workers at the recruitment sites. SARS-CoV-2 antibody testing was done using a rapid point-of care SARS-CoV-2 antibody test (POCT) and participants were invited to fill in a questionnaire (supplementary file 1) in collaboration with a trained project employee, at the same time as the test was performed. We recruited participants from 21 sites in the four biggest cities in Denmark; Copenhagen, Aarhus, Aalborg and Odense. The recruitment sites were shelters, supervised sites for intravenous drug abusers, food distribution sites, meeting places and day/night cafés. In the week prior to our visit, written information was distributed by shelter workers at the recruitment sites notifying the participants of our project. To ensure a high attendance and inclusion, recruitment sites were visited several times, on different days and at different time of the day, including weekends and evenings. The objective was to invite all PEH and shelter workers at each of the 21 assessed sites to participate. However, participation was voluntary. Participants were encouraged to wait around for15 minutes for their test results, but in case they did not want to, they were contacted if the test result was positive. Most came back throughout the day to receive their test result.

Background population

All Danish blood donations are routinely screened for the presence of SARS-CoV-2 antibodies since October 2020. Blood donations take place in all five Danish administrative regions [18] and donors are 17–69 years old. The seroprevalence estimates from the period between the 1st and 22nd of November 2020, are used in this study as proxies for the SARS-CoV-2 infection in the background population.

Point of care test

SARS-CoV-2 antibodies in PEH and shelter workers were detected in whole blood, by use of the OnSite COVID-19 IgG/IgM Rapid Test (CTK Biotech inc., Poway, California, United States of America) according to the manufacturer’s recommendations. This POCT is a single use lateral flow chromatographic immunoassay rapid test, intended for qualitative detection and differentiation of SARS-CoV-2 immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies. Blood was extracted from the fingertip. Test results were read after 15 min by a trained project employee. Participants were categorized as seropositive if they were either IgG and/or IgM positive. The test sensitivity and specificity is 96.86% (95% confidence interval (95% CI:93.66%-98.47%) and 99.39% (95% CI 97.80%-99.83%) respectively[19], as reported from the manufacturer. In house validation of CTK’s POCT showed a slightly lower sensitivity of 90% and a specificity of 100% [20].

Enzyme-Linked Immunosorbent Assay (ELISA)

In contrast to PEH and shelter workers, blood donors were screened for seropositivity using an Enzyme-Linked Immunosorbent Assay (ELISA), with a sensitivity of 96.7% (95% CI 92.4–98.6) and specificity of 99.5% (95% CI 98.7–99.8) (Beijing Wantai Biological Pharmacy Enterprise, China). Participants who were either IgG and/or IgM positive, were categorized as seropositive.

Questionnaire

Participants were invited to fill in a questionnaire provided at the recruitment site, comprised of questions covering sociodemographic characteristics, physical health, self-reported height and weight, use of drugs and alcohol, sex work, co-morbidities, symptom manifestations and the use of personal protective equipment against SARS-CoV-2. Alcohol abuse was defined as drinking more than the national recommendations of < 1 or < 2 beverages per day for women and men, respectively [21]. The questionnaire was filled out with the assistance of a trained project employee. Personal data was collected using a web-based electronic data capture tool (Research Electronic Data Capture, REDCap) [22, 23].

Study group

PEH were defined as people living rough, in emergency accommodation, in accommodation for the homeless or living in non-conventional dwellings due to lack of housing, in accordance with the ETHOS (European Typology on Homelessness and Housing Exclusion) classification [24] established by the FEANTSA (European Federation of National Organizations Working with the Homeless) [25]. Sex workers were defined as individuals either having reported to be engaged in sex work in the questionnaire and/or being included at one of the designated safe havens for sex workers, Reden or Reden International [26]. The safe havens are drop-in centers that offer sex workers a break from the streets, providing food, counselling and a free and anonymous health clinic. Shelter workers were defined as people either working or volunteering at the recruitment sites, by providing support to the residents and visitors [27].

Primary outcome

The primary outcome was the proportion of the study population with a positive antibody test (IgG and/or IgM) for SARS-CoV-2.

Statistical analysis

The estimated sample size was 491, as we assumed that the risk of being seropositive were two times higher for PEH compared to the estimated 3% seroprevalence in the background population. This was calculated with an α of 0.05 and power of 80%. Seropositivity is presented as numbers (n) and percentage (%) with 95% confidence intervals (95% CI). Baseline characteristics and exposures are presented as n (%) for factors and median and interquartile range (IQR)) for numeric variables as appropriate. The body mass index (BMI) was defined as weight in kilograms divided by height in meters squared. BMI was calculated from self-reported height and weight. We calculated the true seroprevalence for both the POCT and ELISA, by adjusting the apparent (crude) seroprevalence in our study group for the sensitivity and specificity of the POCT and ELISA. The calculations were done using the epiR package and by use of the method suggested by Rogan and Gladen to calculate a true prevalence with a 95% CI adjusted for sensitivity and specificity of a test [28]. Possible associations between exposures and primary outcome were reported as odds ratios (OR) and presented with 95% CI. Answers with “do not know” were classified as missing and answers marked “not relevant” were classified as “no”. We tested for significance using Fischer’s exact test. Significant risk factors of seropositivity among sex workers were combined in a multivariate logistic regression model including region of stay, age and gender. P-values < 0.05 were considered significant. Analyses were performed using RStudio version 1.2.5001 [29].

Results

Characteristics

We recruited a total of 827 participants between the 2nd and 20th of November 2020 of which 819 provided their SARS-CoV-2 antibody result as illustrated in Fig. 1. Participants were recruited from 21 recruitment sites placed in Copenhagen (n = 351), Odense (n = 128), Aarhus (n = 144) and Aalborg (n = 144). Fifty-two participants did not register their test location. The recruited participants included 628 PEH (median age 50.8 (IQR 40.9–59.1) years, 35.5% female) and 191 shelter workers (median age 46.6 (IQR 36.1–55.0) years, 74.5% female). Baseline characteristics of the cohort are shown in Table 1, stratified first on PEH and shelter workers and later according to seropositivity. The PEH were older (p < 0.001), had a lower BMI (p = 0.02), were more likely to be male (p < 0.001) and smoke (p < 0.001) compared to shelter workers. The only significant difference between the seropositive and seronegative participants, was significantly higher BMI among the seropositive group (p < 0.001).

Fig. 1
figure 1

Flow chart reporting the flow of participants through each stage

Table 1 Baseline characteristics of the study cohort, stratified by shelter workers and people experiencing homelessness (PEH) and by seropositive or seronegative status

Seroprevalence

Of the 819 participants, 55 (6.7%) were seropositive. We found that 43 of 628 (6.8%) PEH and 12 of 191 (6.3%) shelter workers were seropositive, the prevalence in the two groups was not significantly different (p = 0.87).

Seroprevalence compared to the background population

In the period between the 1st and 22nd of November 2020, the background population (n = 18,505) had a 2.9% seroprevalence. This group was characterized by a median age of 43 (IQR 29–54) years and a higher proportion (47.9%) were women. Taken together, the participants in our study (PEH and shelter workers combined) were at a significantly higher risk of seropositivity than the background population (OR 2.37, 95%CI 1.75–3.17, p < 0.001). Figure 2 illustrates the regional SARS-CoV-2 seroprevalence in PEH, shelter workers and the background population. The seroprevalence among PEH alone was also significantly higher than that in the background population (OR 2.43, 95%CI 1.72–3.35, p < 0.001). The subset of shelter workers (n = 191) in general was significantly associated with seropositivity compared to the background population (OR 2.21, 95%CI 1.12–4.00). In an adjusted analysis for test characteristics of the POCT we found an estimated true seroprevalence of 7.46% (95% CI 5.77–9.60). The estimated true seroprevalence as measured by ELISA was 2.50% (95%CI 2.26–2.76).

Fig. 2
figure 2

Seroprevalence among PEH and shelter workers, compared to the background population

Risk factors for attracting infection

Table 2 shows the prevalence of risk factors (drugs, alcohol, sex work) in the study population, stratified by seropositivity. Tobacco was not considered a drug. None of the lifestyle risk factors were significantly associated to seropositivity. A total of 285 (45.4%) PEH reported use of drugs, the most commonly used were cocaine (18.6%) and heroin (15.6%). Among the seropositive PEH, heroin (14.5%) was the most common drug while the seronegative PEH were more likely to use cocaine (16.9%). Further, we found that 326 (51.9%) PEH were smokers; of those 326 (51.9%) smoked tobacco and 180 (28.7%) smoked cannabinoids. Other reported smoked substances were cocaine (3.5%) and heroin (2.5%). A total of 307 (51.5%) PEH reported some use of alcohol within the past year. Of the 191 shelter workers only 3 (1.6%) reported using drugs, all of which were of cannabinoids. Further, 166 (89.2%) reported any use of alcohol within the past year. PEH were significantly more likely to abuse alcohol (p < 0.001) and use drugs (p < 0.001), compared to the shelter staff.

Table 2 Characteristics and risk factors stratified according to seropositivity stratified by PEH and shelter worker (IgM and/or IgG)

Sex work

Of the 72 (11.5%) PEH who engaged in sex work, 8 (11.1%) were seropositive. Table 3 illustrates characteristics and risk factors of PEH stratified by sex work. Sex workers were younger, more likely to be female and less likely to report smoking and/or IV drug use than other PEH (all p < 0.001). Further, two (1.0%) shelter workers reported having engaged in sex work, of whom one was found to be SARS-CoV-2 seropositive. The seroprevalence among all participants, both PEH and shelter workers, engaging in sex work (n = 74) was 12.2%. Sex workers were 1.9 times more likely to be seropositive compared to non sex workers (OR 1.9). For all participants engaging in sex work (n = 74), there was a significantly increased risk of seropositivity for IgG antibodies compared to the rest of the study group (OR 3.00, 95%CI 1.06–7.49, p = 0.02). However, for the combined seropositivity (IgG and/or IgM antibodies) the difference did not reach significance (OR 2.10, 95%CI 0.87–4.60, p = 0.08).

Table 3 Characteristics and risk factors for people experiencing homelessness (PEH), stratified by engagement in sex work

We included a total of 33 shelter workers working at designated safe havens for sex workers, of whom 4 (12.1%) were found to be seropositive. Shelter workers at designated safe havens for sex workers had a far greater infection rate than other shelter workers, however, the difference was not significant (OR 2.57, 95%CI 0.53–10.38, p = 0.13). Further, there was no difference in seropositivity between shelter workers at designated safe havens (12.1%) and all sex workers (12.2%) (OR 1.38, 95%CI 0.32–5.26, p = 0.752). When combining all participants who reported sex work or were recruited at designated safe havens, shelter workers and sex workers (n = 107), we found a significantly increased risk of seropositivity (IgG and/or IgM antibodies) compared to other participants (OR 2.23, 95%CI 1.06–4.43, p = 0.02). In a multivariate logistic regression model of region of stay (supplementary table 1), being a sex worker or working at a designated safe haven remained a significant risk factor of seropositivity compared to PEH who does not engage in sex work (OR 2.4, 95% CI 1.12–4.70, p = 0.02).

Symptoms and self-reported illness

Of the 628 PEH, 57 (9.1%) suspected previous infection with SARS-CoV-2 of whom 8 (14%) were seropositive. A total of 371 (59.1%) PEH reported being previous tested for SARS-CoV-2, of whom 11 (3.0%) were reportedly positive at the time. However, of those only 3 (27.3%) tested positive for SARS-CoV-2 antibodies in our study. Of the shelter workers, 154 (80.6%) reported previous testing for SARS-CoV-2. No shelter workers reported having previously tested positive for SARS-CoV-2, but 21 (11%) suspected previous infection with SARS-CoV-2 of whom 5 (23.8%) were seropositive.

Seventeen of 43 (39.5%) seropositive PEH and 10 of the 12 (83.3%) seropositive shelter workers reported having had symptoms. Overall, a total of 441 (53.8%) participants in our study group reported having had one or more symptoms. Of those, 15 (3.3%) reported being hospitalized at the time of their symptoms. Among the PEH 303 (48.2%) reported experiencing one or more symptom, of whom 26 (8.6%) though it was attributable to SARS-CoV-2. Of the shelter workers 138 (72.3%) reported one or more symptom, and of those 20 (14.5%) thought the symptoms were attributable to SARS-CoV-2; significantly more than among the PEH (OR 2.79, 95%CI 1.94–4.06, p < 0.001). Similarly, shelter workers were more likely than PEH to have more than three symptoms (OR 2.11, 95%CI 1.47–3.03, p < 0.001). The most common symptoms reported in the combined cohort were fever (23.2%) and shivers (18.5%). However, there was no observed significant association between experiencing symptoms and seropositivity (OR 0.82, 95%CI 0.45–1.47, p = 0.49). Table 4 shows symptoms stratified by seropositivity. Symptoms in PEH and shelter workers is illustrated in supplementary table 2.

Table 4 Symptoms reported by the participants, stratified by serology findings

Use of protective means against SARS-CoV-2 infection

Figure 3 shows use of protective means against SARS-CoV-2 in PEH compared to shelter workers. Only 25 (4%) of the PEH reported that they did not follow any of the recommended guidelines. Among the shelter workers only 3 (1.6%) reported not following the guidelines. The supplementary Fig. 1 illustrates use of protective means against SARS-CoV-2 in seropositive and seronegative participants. There was no significant association between following any given guideline and seropositivity.

Fig. 3
figure 3

Percentage of PEH and shelter workers who follow the national SARS-CoV-2 measures and guidelines

Discussion

To our knowledge, our study is the first to investigate and evaluate the nationwide prevalence of SARS-CoV-2 infection among PEH. We found that the seroprevalence among PEH was twice that of the background population. Furthermore, sex workers and shelter workers at designated safe havens were at increased risk of infection with SARS-CoV-2, independent of region. We found that seropositive PEH were less likely to report symptoms, compared to seropositive shelter workers. The results further suggest that almost all PEH follow one or more national SARS-CoV-2 prevention measure, as illustrated in Fig. 3. The high seroprevalence among PEH found in our study suggest that PEH are at elevated risk of infection with SARS-CoV-2 which could be taken into consideration when deciding in which phase they are eligible for a vaccine, as part of the national SARS-CoV-2 vaccination program rollout. Our findings are consistent with findings in previous studies[4, 30,31,32], with elevated prevalence of SARS-CoV-2 for people living in precarious conditions, relative to the background population. This is consistent with living in overcrowded conditions, a main risk factor associated with infection of SARS-CoV-2. The observed increased seroprevalence in sex workers compared to those who did not engage in sex work, is in accordance with the recent statement from UNAIDS (the joint United Nations Programme on HIV/AIDS) which emphasized how sex workers are risking their health by working during the current SARS-CoV-2 pandemic in order to provide for themselves [33]. Further, we believe that national SARS-CoV-2 measures such as social distancing are simply not feasible for sex workers as their work requires some level of close physical contact with clients and self-isolation could result in a total loss of income. These results show that the risk of contracting SARS-CoV-2 should be added to the risks experienced by sex workers.

In previous studies around half the seropositive participants are reporting symptoms attributable to SARS-CoV-2 [34,35,36]. Symptom prevalence in our study is consistent with previous findings on PEH and implies a high proportion of asymptomatic infections [4, 30, 37, 38]. Additional explanations might include difficulties in recalling previous symptoms and differentiating symptoms attributable to substance abuse and SARS-CoV-2. Thus, symptom assessment in PEH might not be predictive for SARS-CoV-2. The high prevalence of substance abuse among PEH is consistent with previous national findings on PEH [7]. Recent studies suggest that suffering from a substance use disorder increases the risk of contracting SARS-CoV-2, while also facing a worse outcome than the background population [3, 39]. We found that seropositive participants had a higher BMI compared to seronegative participants. The seropositive participants in our study were generally overweight [40], with a mean BMI of 27.7. The Danish Health Authority has previously declared individuals who are overweight at increased risk of developing severe illness from SARS-CoV-2. They identify multiple possible reasons as to why overweight individuals are at increased risk, counting impaired immune function, comorbidities and low physical activity levels [11].

Strengths and Limitations

Our study has several limitations. First, the cross-sectional study designs do not allow determination of time of infection nor provide information on when participants became seropositive. Individuals who might already have tested positive by PCR at an earlier point in time, might have chosen not to participate in this study. Individuals anticipating a positive result might have chosen not to participate, fearful of the consequences such as isolation. If so, our results could be biased and the seroprevalence be underestimated. However, our impression is that the desire to participate and get tested was high (9.8% of an estimated 6 431 homeless people in Denmark). Of the 827 participants in our study group, 544 (67.0%) reported having previously been tested (nasopharyngeal swab and/or antibody test) and still participated. Further, questions on sociodemographic characteristics, physical health, use of drugs and alcohol, co-morbidities, symptom manifestations and the use of personal protective equipment against SARS-CoV-2 were self-reported, therefore an information bias could have affected our results.

We compared our seropositivity findings to that of blood donors serving as a proxy to the background population, with some limitations to consider. First, blood donors are required to have a good general health and are ineligible to donate blood if they have ever engaged in sex work, have certain medical conditions, travel to certain international destinations and/or receive certain immunizations. Seropositivity could as a result potentially be lower among blood donors compared to the background population. However, due to their good health, blood donors are more represented in the labor market and so more at risk of SARS-CoV-2 infection.

Another limitation is that blood donors and our study group were tested using different methods, POCT vs ELISA however both with high and similar sensitivity and specificity. Serology testing with a POCT was chosen for this study as it only requires capillary blood obtained by a finger prick, can be performed as a self-test and provides quick results. We expected that testing by POCT would increase the number of participants, due to the above-mentioned circumstances. Another strength of the POCT is that by not requiring a venous blood sample nor laboratory equipment, it is less costly than a serology test performed in the laboratory and provide more rapid results. Furthermore, communicating test results obtained from an ELISA test would not be possible in the examined group of PEH.

A limitation to the serology approach of this study versus test by PCR, is that the measured antibodies generated in response to SARS-CoV-2 exposure are generated in the weeks after the acute phase of the infection. Recent studies have shown that SARS-CoV-2 IgM antibodies reach a detection threshold 5–7 days after symptom onset [25]. Thus, participants currently or recently infected with SARS-CoV-2 might not have been identified in this study. A strength of serology approach versus test by PCR is that it allows us to detect SARS-CoV-2 antibodies in those categorized as asymptomatic carriers and those with suspected SARS-CoV-2 despite negative PCR results. Our study sought to investigate the seroprevalence among all PEH and risk factors of being seropositive. We did not have the sample size to look at risk factors in subgroups, such as sex workers. Future studies should investigate specific risk factors among these at-risk groups.

Conclusions

In this study we found a high SARS-CoV-2 seroprevalence among PEH and shelter workers, compared to the background population. There was no significant association between reported symptoms and seropositivity, nor between substance abuse and seropositivity. The results from this study could be taken into consideration when deciding in which phase PEH are eligible for a vaccine, as part of the national SARS-CoV-2 vaccination program rollout. Furthermore, our findings could be of great value in case of future pandemics.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank Kåre Mølbak, Thomas Benfield and Cyril Martel for their contributions to this study. The authors would also like to thank Mændenes Hjem, Café Klare, Herbergscentret Sundholm, Svenstrupgaard re-establishment centre, Jægergårdsgade drop-in centre, Reden (International, Copenhagen, Odense, Aarhus and Aalborg), Redernes Krisecenter and DanChurchSocial’s the Compass, Mariatjenesten, Hillerødgade, shelter Aalborg, shelter Aarhus, shelter Nørregade and shelter Østergade

Funding

This study was supported by grants from the foundations of TrygFonden [152144] and HelseFonden [20-A-0124]. The funders did not influence study design, conduct or reporting.

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Authors

Contributions

The study was designed and initiated by KI, RS and KF. Data was collected by: ARRE and KF. Data analysis was done by ARRE and RBH. The first draft was written by ARRE, KF, RBH, HB and KI. All authors have critically revised the manuscript and agree to be accountable for all aspects of the work. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Alexandra R Röthlin Eriksen.

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Ethics approval and consent to participate

This study was carried out in agreement with the Helsinki II declaration. All participation in this study was voluntary. This study was registered with the Danish Data Protection Authorities (P-2020–901). All personal data, obtained in REDCap, was kept in accordance with the general data protection regulation and data protection law stated by the Danish Data Protection Agency.

This study was performed as a national surveillance study under the authority task of Statens Serum Institut (SSI; Copenhagen, Denmark, the Danish National Institute for Infectious Disease Control and Prevention which performs the epidemiological surveillance of infectious diseases for the Danish government) and therefore, according to Danish law, does not require any formal approval from an ethics committee. Due to the waiver of consent granted by the Ethics Committee of the Capital Region in Denmark (20057075), participants were not asked for formal consent at any stage.

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Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Eriksen, A.R.R., Fogh, K., Hasselbalch, R.B. et al. SARS-CoV-2 antibody prevalence among homeless people and shelter workers in Denmark: a nationwide cross-sectional study. BMC Public Health 22, 1261 (2022). https://doi.org/10.1186/s12889-022-13642-7

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