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The Study of the Prevention of Anal Cancer (SPANC): design and methods of a three-year prospective cohort study
- Dorothy A Machalek1, 11,
- Andrew E Grulich1,
- Richard J Hillman2,
- Fengyi Jin1,
- David J Templeton1, 3,
- Sepehr N Tabrizi4, 5, 6,
- Suzanne M Garland4, 5, 6,
- Garrett Prestage1,
- Kirsten McCaffery7,
- Kirsten Howard7,
- Winnie Tong8,
- Christopher K Fairley9,
- Jennifer Roberts10,
- Annabelle Farnsworth10 and
- I Mary Poynten1Email author
© Machalek et al.; licensee BioMed Central Ltd. 2013
Received: 1 October 2013
Accepted: 7 October 2013
Published: 9 October 2013
The incidence of human papillomavirus (HPV)-associated anal cancer is increasing in men who have sex with men (MSM). Screening for the presumed cancer precursor, high-grade anal squamous intraepithelial lesions (HSIL) in a manner analogous to cervical cancer screening has been proposed. Uncertainty remains regarding anal HPV natural history and the role of anal cytology and high-resolution anoscopy (HRA) as screening tests. Well-designed cohort studies are required to address these issues.
The SPANC study is a prospective study of the epidemiology of low-risk and high-risk anal HPV infection and related cytological and histological abnormalities in HIV-negative and HIV-positive homosexual men aged 35 years and over. The study aims to recruit 600 men from community-based settings in Sydney, Australia. There are six study visits over three years. At the first five visits men undergo a digital ano-rectal examination (DARE), an anal “Papanicolaou” (Pap) test for HPV detection, genotyping and anal cytology, followed by HRA and directed biopsy of any visible abnormalities. The men also complete a behavioural questionnaire before each visit. Questions include a detailed history of sexual behaviour, of anal symptoms, possible anal cancer risk factors and validated quality of life and psychosocial questions. Questionnaires are also completed 2 weeks and 3 months following the provision of test results and include questions on participant experience during the procedure and post-procedure symptoms, including pain and bleeding in addition to quality of life/ psychosocial outcomes.
Recruitment for the study began in September 2010 and will conclude in mid-2015, with follow up continuing to 2018. Thus far, over 350 men have been recruited from a variety of community-based settings and are broadly representative of the target screening population. The SPANC study is one of only a small number of cohort studies globally to perform HPV, cytology and HRA screening on all participants over multiple time points. The study results will contribute to understanding of the natural history of anal HPV and inform the possible development of guidelines for implementing anal cancer screening programs in this population.
More than 80% of squamous cell anal cancers are caused by infection with high-risk human papillomavirus (Hr-HPV), mainly HPV16 . In the general population anal cancer is uncommon, with rates of between 1 and 2 cases per 100,000 per year in most settings . However, certain subpopulations are at increased risk of this disease. These include; 1) women who have had previous HPV-associated anogenital (i.e. vulval, vaginal or cervical) HSIL or cancer ; 2) people with immune deficiency, including those with HIV infection and organ transplant recipients ; and 3) men who have sex with men (MSM) . Anal cancer rates are highest in MSM , especially in HIV-positive MSM, with no evidence of decline in incidence since the introduction of effective antiretroviral therapy .
Prophylactic vaccination against HPV has enormous potential to prevent anal cancer among high-risk populations in the future [8, 9]. However, current adult populations are unlikely to benefit from this. In view of the increasing health burden of anal cancer and its biological similarities to cervical cancer, some researchers have advocated for the introduction of an anal cancer screening program for high-risk populations . There is uncertainty though, regarding anal HPV natural history, especially concerning rates of progression and regression of the presumed anal cancer precursor, high-grade squamous intraepithelial lesions (HSIL). There is ongoing assessment and discussion around the performance of anal cytology. In most countries, there is a scarcity of specialist clinicians trained in high resolution anoscopy (HRA). Furthermore, the safety and efficacy of available treatment options is yet to be proven in randomised controlled trials . For these reasons, despite years of advocacy, no national authorities recommend a cytology-based screening program for anal cancer in any high-risk population .
Little is known about the psychosocial and quality of life (QoL) impacts of anal cancer screening. The psychological and QoL burden of anal cancer cytology screening among homosexual men is especially important since a large number of men will have abnormalities detected at screening . In women, there is strong evidence that psychological wellbeing, QoL and psychosexual health are negatively affected by the experience of an abnormal cervical screening and/or HPV test result . It is currently unknown how people will respond to an anal cancer screening program and research examining the psychosocial consequences of screening is needed.
The Study of the Prevention of Anal Cancer (SPANC) is a longitudinal study exploring the epidemiology of anal HPV infection and related abnormalities among a community recruited cohort of homosexual men. The study results will contribute to our understanding of the natural history of the disease, the psychological well-being and QoL impact and the health-related costs of anal cancer screening of homosexual men. This paper describes the main design and methodological aspects of the study and includes descriptive data on selected baseline characteristics for the first 350 participants recruited from September 2010 to the end of July 2013.
Design and methods
Overview of design
SPANC is an ongoing, prospective, cohort study based in Sydney, Australia. The study follows HIV-negative and HIV-positive homosexual men aged 35 years and older at six visits over a period of three years, including a baseline enrollment visit, four follow-up screening visits and final visit to discuss all study results. The main objective of the study is to investigate the epidemiology of anal HPV infection and related cytological and histological anal abnormalities in this population. Specifically, the study aims to 1) determine the prevalence, incidence and risk factors for anal HPV genotype detection and associated lesions, 2) investigate rates of clearance and persistence of anal HPV infection, 3) investigate rates of progression and regression of intraepithelial anal lesions and 4) assess the psychosocial/QoL impact of screening in homosexual men. In addition to the objectives described above, a number of sub-studies are nested within the main study. These include sub-studies on molecular biomarkers, lesion-specific HPV genotyping using laser capture microdissection (LCM), HPV serology, immunology, health economics and qualitative studies. Details of these sub-studies will not be covered in this paper.
The baseline data collection commenced in September 2010 and will conclude when the full study sample of 600 men is enrolled (approximately July 2015). Data from the baseline phase of the study will be used to investigate the prevalence of type-specific anal HPV infection and prevalence of anal squamous intraepithelial lesions. Results from the baseline questionnaire will be used to investigate the demographic, behavioural and clinical determinants and risk factors for type-specific HPV infection and low and high-grade anal squamous intraepithelial lesions (LSIL and HSIL). Additionally, the baseline cytology and HPV genotyping results will be compared with the final histological diagnosis to evaluate the value of anal cytology and HPV genotyping as potential screening tools in a setting where the prevalence of anal HPV and HSIL is very high . Baseline psychosocial factors will be compared with post-test questionnaire findings.
Follow-up of participants will continue to 2018. Incidence, persistence, clearance and regression rates of type-specific HPV infection and associated HSIL will be calculated and compared between groups with different behavioural, biological and clinical risk factors. Additionally, the impact of screening on men’s physical and psychological well-being and QoL will be measured and used to assess the benefits and potential harms of the screening test.
The study will recruit a total of 600 men with an approximate 3:2 ratio of HIV-negative to HIV-positive participants. Interim baseline data will be published on the first 350 participants recruited to the end of July 2013.
Ethics approval for the study was first granted on 21 April 2010 by the St Vincent’s Hospital (SVH, Sydney, Australia) Human Research Ethics Committee (File Number 09/203). The study is conducted in accordance with the National Health and Medical Research Council (NHMRC) National Statement of Ethical Conduct in Human Research 2007 (http://www.nhmrc.gov.au/book/national-statement-ethical-conduct-human-research), the World Medical Association Declaration of Helsinki (October 2000) and the International Conference on Harmonisation Note for Guidance on Good Clinical Practice (ICH-GCP) (http://www.tga.gov.au/industry/clinical-trials-note-ich13595.htm). Written informed consent is obtained from all individuals before any study-specific procedures are performed.
Study population and recruitment
Obtaining a representative sample of homosexual men is challenging, as there is no defined population from which to draw a sample. Therefore, recruitment strategies used in SPANC are based on previous community-based studies conducted by the Kirby Institute and are centered on a number of community-based settings in Sydney. These include gay community social events and organizations, referrals from other participants and participants from previous community-based studies involving homosexual men at the Kirby Institute. Information regarding the study is distributed through leaflets and advertisements on gay community websites and SPANC information nights. To ensure a diverse population of men is reached, advertisements are also placed in the local gay press and the social networking site Facebook.
Eligible participants are men aged 35 years or over who report sex with another man in their lifetime (any homosexual contact). Participants are excluded if they are unable to understand English, unable to attend scheduled follow-up visits or are unwilling to undergo HRA, have a bleeding disorder or are on anti-coagulation medications (except aspirin and other non-steroidal anti-inflammatory drugs). Men who have previously had an HRA or who have a history of anal cancer are also excluded from the study.
Once enrolled, contact with the participants is maintained though regular reminder emails, text messages and telephone calls from SPANC study staff. To maximize retention and maintain engagement with the cohort, participants receive regular SPANC newsletters containing information on the progress of the study as well as general information relevant to the field. The newsletters are also used to address issues and concerns raised by the participants at their study visits or in the feedback section of the follow-up questionnaires.
Study visit schedule outlining procedures and assessments performed at each visit
Follow-up (+/− 3 months)
Follow up Interview
Digital ano-rectal exam
Urine sample & throat swab1
High resolution anoscopy & biopsy
Discussion of all study results and anal cancer risk
Syphilis testing 1
Gonorrhoea and chlamydia testing1
Data collected in the baseline questionnaire (visit #1) includes data on socio-demographics, predictors of gay social engagement and community involvement, lifetime and recent (previous six months) sexual practices, general health, lifestyle and anal health. At follow-up visits (#2 - #5) the questions focus on sexual experiences, general health, lifestyle and anal health issues in the previous six months. Additional information is sought from HIV-positive participants at all study visits on clinical measures such as CD4 T-lymphocyte count, HIV viral load and AIDS-defining illnesses. Measures of the potential psychosocial impact of anal screening are collected at the baseline visit and in the two-week and three-month (post-visit) questionnaires. The two-week questionnaire also contains questions on discomfort experienced during the procedure and post-procedure symptoms, including pain and bleeding.
Summary of the main sections of the baseline and follow up questionnaires
Examples of variables
Measures and standardized scales
Age, country of birth, ethnicity, relationship status, accommodation status, employment status, income
Age at first receptive anal intercourse:
Number of lifetime insertive and receptive anal partners (with and without a condom)
Lifetime number of female partners
Preferred position during anal sex (receptive or insertive)
Number of insertive and receptive anal partners (with and without a condom) in the previous six months
Frequency of fingering, fisting and insertion of toys and other objects in the previous six months
Gay social engagement
Time spent with gay/homosexual friends
Gay community periodic surveys 
Disclosure of sexuality to friends/family
Number of gay friends
Involvement with gay community
Health and lifestyle
45 and Up study 
Alcohol and drug use
History of health problems (lifetime and in the last month)
History of depression and anxiety
Examples of Variables
Measures and Standardized Scales
History and frequency of anal douching
Adapted from the Blokes with Anal Squamous Intraepithelial Lesions (BASIL) study 
History of STIs
Generic measure of health related quality of life
SF36 v2 (Australian) 
Disease specific distress associated with diagnosis of SIL
Generic measures of distress
Distress thermometer 
Worry about developing anal cancer Perception of risk of anal cancer
Cancer worry 
Frequency of thinking about the tests
Perceived risk of cancer Intrusive thoughts 
HIV-positive status specific
Length of HIV infection
Positive Health study 
Most recent CD4-Tcell count
Nadir CD4-Tcell count
Type of antiretroviral medication
Length of time on antiretroviral medication
History of AIDS associated illness
Procedure related feedback
Post procedure pain and bleeding
Length of pain and bleeding
Discomfort associated with the procedures
Reason for joining study
Source of recruitment
Blood samples are drawn for HIV and syphilis testing. Participants provide additional, optional consent to have their blood stored (as serum and cell pellets). HPV genotype specific serology may be performed and specimens may be tested for other potential determinants of anal cancer risk at a later stage.
Urine and throat swab
Swabs from the tonsillar arch and posterior nasopharynx are collected for gonorrhoea testing. For urethral gonorrhoea and chlamydia testing participants are asked to void the first 10 ml of urine into the sterile container provided.
Digital Ano-Rectal Examination (DARE)
DARE is performed on all participants at each study visit by a study physician using lubricant mixed with 1% lignocaine. Possible abnormalities and their positions in the anal canal or perineum are recorded on the HRA report form (See Additional file 1).
Anal pap test collection
Two anal canal samples are collected from all participants at each study visit. The first is an anal Pap test, wherein a Dacron swab moistened with tap water is inserted (3-5 cm) into the anal canal by the study clinician, pressed firmly against the wall of the anal canal and slowly withdrawn in a spiral fashion over approximately one minute. Immediately after sampling, the swab is inserted into a vial containing 20 ml of PreservCyt (Hologic, Inc., Marlborough, MA, United States) fixative medium and mixed vigorously. Specimens are sent to Douglas Hanly Moir Pathology (DHM, Sydney, Australia) for processing. Before cytological processing, one fifth (4 mL) of the PreservCyt sample is transferred into a second vial for HPV DNA detection and genotyping to avoid cross-contamination . The remaining sample is used for cytological analysis. If the anal Pap test is unsatisfactory for cytological evaluation, a repeat anal collection is performed no less than two weeks from the first. This minimum time requirement is to allow sufficient time for the anal mucosal cells to regenerate after the initial Pap test.
A second swab (BD ProbeTec sample collection kit, BD Diagnostics, Sparks, MD, United States) is inserted (3-5 cm) into the anal canal by the study clinician and gently rotated, then placed into the sterile transport tube for testing for gonorrhoea and chlamydia.
High Resolution Anoscopy (HRA)
HRA is performed at each study visit following collection of the anal canal samples. After a visual examination of the perianal region and perineum, a plastic anoscope coated with lignocaine-containing lubricant is inserted into the anal canal and the introducer removed. A gauze swab soaked in 3% acetic acid is then inserted through the anoscope and left in the anal canal for one minute, to stain the anal mucosa. The gauze is then removed and the anoscope re-inserted. The anal canal is visualised under high magnification using a colposcope with real-time video image capturing software (Second Opinion Version 7.0, Second Opinion Telemedicine Solutions, Inc. Torrance, California, USA). Further acetic acid is repeatedly applied, followed by Lugol’s iodine to stain for any macroscopic abnormality [27, 28]. Biopsies of visual abnormalities suspicious of HPV-related lesions are taken with mini Tischler forceps and placed into a formalin/saline solution and sent to DHM for processing and histopathological assessment. Separate forceps are used for each lesion, in order to prevent cross contamination.
Following removal of the anoscope, 3% acetic acid is applied to the perianal skin and the area examined using the colposcope. If both intra-anal and peri-anal biopsies are to be taken, the anoscopist may schedule the participant to return for peri-anal biopsies at a later visit (within two weeks of study visit). All peri-anal abnormalities are biopsied under lignocaine local anaesthesia.
Clinical impressions of intra-anal and peri-anal abnormalities and their anatomical positions are recorded on the HRA report form (See Additional file 1). Quantification of the total area of intra-anal and/or peri-anal tissue affected by HSIL is recorded retrospectively once the histology results are known using a seven point scale; 1) Single small lesion, 2) < half of one quadrant, 3) ≥ half of one quadrant but < one quadrant, 4) ≥ one quadrant but < two quadrants, 5) ≥ two quadrants but < three quadrants, 6) ≥ three quadrants, 7) circumferential disease. Additionally, annotations of suspected HSIL lesions are taken at the time of the procedure. All reporting is performed with the knowledge of the participant’s HIV status. The clinicians are also aware of the participant’s cytology, and HRA +/− biopsy results from any previous study visits.
All study anoscopists are required to undergo a formal training program prior to their involvement with the study. This training program was adapted from the British Society for Colposcopy and Cervical Pathology Training Program . Anoscopists are required to observe at least 25 HRA procedures and then perform at least 50 HRAs under the direct supervision of an experienced anoscopist. All cases are recorded in a log book and the results of anal Pap tests and biopsies reviewed, when they become available. Once the anoscopist is deemed competent, they start performing HRA on their own. If required, they are able to access advice and clinical support from an experienced anoscopist on an ongoing basis and the log book of all procedures is maintained.
A quality assurance program for anoscopists is also in place. First, each anoscopist’s cytology/histology correlation and number of unsatisfactory Pap tests are summarised quarterly and a meeting of anoscopists is held at which the data are discussed and any significant differences between anoscopists are addressed. Second, a SPANC multidisciplinary team meeting is held quarterly involving the study investigators, anoscopists and pathologists. Six to eight cases are covered at each meeting including review of HRA images, cytology and histology slides and results, and HPV results. Each anoscopist presents their cases and any discordant results are discussed by the team. Detailed minutes are kept and action plans determined.
All anal swabs placed in PreservCyt specimens are tested for HPV DNA using the Roche LINEAR ARRAY (LA) HPV genotyping test (Roche Molecular Systems, Alameda, CA, United States). DNA is extracted with the automated MagNA Pure isolation and purification system (Roche Molecular Systems) by a modified protocol using 1 ml aliquots of PreservCyt specimens as described previously . The LA HPV genotyping test involves PCR amplification of a 450 bp region of the HPV L1 gene and allows for the identification of 37 HPV genotypes (6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 64, 66, 67, 68, 69, 70, 71, 72, 73, 81, 82, 83, 84, IS39 (HPV 82v subtype), and CP6108 (HPV 89) as well as amplification of 265 bp region of the human ß-globin gene, serving as an internal control. As an in-house modification, samples that produced a negative internal control are retested with ½ the volume of eluted DNA in order to reduce the inhibition due to high bacterial DNA content in those samples. In addition, due to possible cross-reactivity of the HPV-52 probe with types 33, 35, and 58 amplicons, samples positive for one or more of HPV-33, 35, and 58 probes are further tested using a real-time PCR assay with an HPV-52 specific hydrolysis probe to rule out presence of HPV 52 in addition to those types .
A screening enzyme immunoassay (EIA; ICE syphilis, Murex Biotech Ltd, Dartford, United Kingdom) is used to test for syphilis. Positive screening results are confirmed with the Treponema pallidum particle agglutination assay and/or the fluorescent treponemal antibody absorption test. The rapid plasma reagin (RPR) test is used on those with confirmed syphilis to assist clinical staging and to detect re-infection. All participants are tested for syphilis at the baseline visit. At visits 3, 4 and 5, serology is performed on those participants who have previously tested negative for syphilis and report sexual activity since the last visit. In participants with confirmed syphilis at any prior visit, only RPR is performed.
HIV testing is performed at baseline on all participants who report their HIV status to be negative, using the AxSYM HIV antigen/antibody Combo (Abbott Diagnostics, Abbott Park, IL, United States). Positive results are confirmed by a western blot assay. HIV testing is performed at visits 3, 4 and 5, on those HIV-negative participants who report sexual activity since the last visit.
Gonorrhoea and chlamydia testing
All baseline samples are tested for gonorrhoea and chlamydia by strand displacement amplification (SDA) using the BD ProbeTec assay (BD Diagnostics, Sparks MD). Positive samples undergo a supplementary nucleic acid amplification assay to confirm the diagnosis. Follow up testing is performed at visit 3, 4, and 5 on those participants who report sexual activity since the last visit.
Cytology and histopathology
The laboratory reporting cytological and histopathological results is a specialist gynaecological unit operating within a large private general pathology laboratory in Sydney, Australia. The gynaecological unit reports more than 250 000 cervical cytology specimens (conventional and liquid-based slides) per year and more than 12 000 gynaecological surgical specimens per year. Three anatomical pathologists, each with more than 15 years’ experience in gynaecological pathology, are involved in the study. The number of cytologists and pathologists involved were limited in order to enhance reproducibility of reporting. Prior to commencement of the study, a pathologist and a cytologist prepared a teaching manual based on available literature and assembled a set of teaching slides, drawn from previous smaller studies of anal canal lesions. All participating staff undertook training based on these materials.
Prior to processing for cytology, a 4 ml aliquot is removed from the ThinPrep vial (total sample volume 20 ml) under sterile conditions. This aliquot is forwarded to Royal Women’s Hospital Molecular Microbiology laboratory (Melbourne, Australia) for HPV testing. The remainder of the sample is processed using a ThinPrep 2000 processor (Hologic Inc.,Marlborough, MA, United States) to produce a TP slide (Hologic Inc., Marlborough, MA, United States), which is then stained with the ThinPrep proprietary stain (Hologic Inc., Marlborough, MA, United States). The slide is manually screened by a study cytologist on a standard light microscope. The cytologist completes a checklist, and creates a provisional report, which is forwarded with the slide to one of the three study pathologists for assessment and final reporting. A ‘satisfactory’ slide is defined as having at least 2000 nucleated squamous cells. The presence or absence of a transformation zone component (at least 10 colorectal glandular cells and/or squamous metaplastic cells) is recorded but does not affect determination of satisfactory status. The Bethesda System (TBS)  criteria and terms are used for cytology reporting. Both the cytologist and pathologist view the slide blind to any knowledge of previous or current cytology or histology. Participant date of birth and study code was available, but no other demographic or clinical information accompanied the specimen.
Biopsies are received in 10% formal saline and processed in a routine fashion. Nine levels of each biopsy are prepared. Levels one to three and seven to nine are routinely stained with Haematoxylin and Eosin (H&E) stain. Levels four to six are prepared on a coated slide and left unstained for potential use for histochemical staining or immunostaining. Each case is viewed and reported by one of the three study pathologists. Every attempt is made to ensure that a different pathologist views the concurrent cytology and histology specimens of an individual participant. At times where this is not possible due to logistical issues, the study pathologist reports the cytology and histology at different times. All reporting is done blinded to clinical factors (other than age) and previous results.
Reporting of the biopsies is performed in accordance with criteria, terminology and recommendations of the Lower Anogenital Squamous Terminology (LAST) Project [32, 33]. Details relevant to this study are as follows:
With respect to non-invasive HPV-related disease, the following terms are used: Negative for Squamous Intraepithelial lesion (SIL); Low-grade SIL (LSIL) and High-grade SIL (HSIL)
Within the category of HSIL, further subdivision is performed, into anal intraepithelial neoplasia (AIN) grade 2 and AIN grade 3. If the biopsy is from perianal skin the terms perianal intraepithelial neoplasia (PAIN) 2 and 3 are used.
Immunostaining for p16 INK4A (p16) is performed on the unstained spare slide in the following circumstances:
To differentiate HSIL from a benign mimic, in particular, atypical immature metaplasia, but also inflammatory/reactive changes, tangential sectioning and partial epithelial denudation.
When a diagnosis of AIN 2 is proposed, in order to confirm positive p16 reactivity. If the result is negative, the lesion is downgraded to LSIL or negative for SIL.
Immunostaining for p16 is not used in formulating diagnoses of straightforward LSIL or straightforward HSIL – AIN 3.
Immunostaining for p16 is reported as positive if there is “continuous strong nuclear or nuclear plus cytoplasmic staining of the basal cell layer with extension upwards involving at least one third of the epithelial thickness” [32, 33]. Any other pattern of staining is regarded as negative.
Carcinomas are measured in two dimensions and categorized as “Superficially invasive squamous cell carcinoma of the anus” (SISSCA) if they meet the LAST definition.
In addition to the LAST categories above, a decision was made to sub-categorise LSIL into ‘exophytic’ and ‘flat’, based on presence or absence respectively of architectural features of condylomata accuminatum. This decision reflected the possibility that the two variations of LSIL may be associated with different HPV types [34, 35].
If infection with organisms other than HPV is suspected, appropriate stains are used, for instance immunoperoxidase staining for HSV-1 and/or HSV-2 in the presence of ulceration; and Ziehl-Neelsen staining if granulomata are present.
Characteristics of the enrolled population
Socio-demographic, health and lifestyle characteristics
HIV positive (n=101)
HIV negative (n=249)
35 – 44 years
45 – 54 years
55 + years
Country of birth
Highest level of education
Tertiary diploma or trade1
Weekly income (AU$)
Currently in a relationship
Yes, with male partner
Yes, with female partner
< once a week
1-2 days a week
3-4 days a week
5-6 days a week
Recreational drug use in the past 6 months 3
Characteristics of HIV-positive men
Nadir CD4 T cell/μL
201 - 500
51 - 200
Last CD4 T cell/μL
Last viral load test
History of AIDS defining illness
Currently taking any anti-retroviral medication
The majority of men were recruited from community based sources including from gay community events (32%) or the SPANC website/Facebook (13%). Fifteen percent (15%) were recruited through friends or partners, 14% through gay organizations or HIV/AIDS organisations, 11% through gay press and 6% other. In 9% of cases men were recruited from general practice or sexual health clinics.
The median age of participants at the time of baseline interview was 49 years (IQR 42–55). The majority of participants (67%) were born in Australia and most (83%) reported Anglo-Celtic ethnic background. The cohort was highly educated, with 57% of participants having completed university and 40% reporting a weekly income of ≥ $1500. Forty-five percent (45%) of men reported being a past or current cigarette smoker. The majority of men (92%) consumed alcohol at least once a week and 73% have used drugs recreationally in the past 6 months (Table 3).
HIV-negative participants in SPANC were generally more likely to have higher levels of education, higher income, having full or part time employment and higher alcohol consumption. HIV-positive participants were more likely to report past or current cigarette smoking and recreational drug use (Table 3).
Almost all participants self-identified as being homosexual, gay or queer (95.4%) and 4.6% self identified as bisexual, heterosexual or other. The median age of first receptive anal intercourse was 21 years (IQR 17–25). Participants were highly sexually active, with 53% of the men reporting more than 200 lifetime male sexual partners, and 29% reported having more than 10 male sexual partners in the previous 6 months. Fifty five percent (55%) of men reported having unprotected receptive anal intercourse (URAI) in the previous 6 months. A lifetime history of anal STIs was also common in this group being reported by 26%, 21%, 17% and 19% of men for anal gonorrhea, syphilis, chlamydia and herpes, respectively. Having ever had anal or genital warts was reported by 43% and 29% of men, respectively. Compared with the HIV-negative, HIV-positive participants in SPANC reported more lifetime and recent sexual partners, more frequent URAI, and more past STIs.
Among HIV-positive participants (n=101), the median time they had been living with HIV was 16 years (IQR 8–25). The majority (84%) had a self-reported CD4 T cell count over 350cells/μL, 79% reported undetectable HIV viral load at their last test and 89% were on treatment at the time of the baseline interview (Table 4).
SPANC is a prospective longitudinal study of the epidemiology of anal HPV and related abnormalities in a community-based cohort of homosexual men aged 35 and older in urban Sydney, Australia. Recruitment for the study began in September 2010 and will conclude in mid-2015, with follow-up continuing to 2018. By July 2013, approximately 404 men had been assessed for inclusion and over 350 men have been recruited into the study. SPANC participants are recruited from a variety of community-based settings and can be considered as broadly representative of the potential target screening population. Men older than 35 years are targeted because anal cancer is still rare at younger ages  and at present, older men are unlikely to benefit from the implementation of HPV vaccination. Thus any future community-based screening program would most likely focus on older homosexual men.
Ultimately, the goal of anal cancer screening will be to identify those pre-cancerous lesions most likely to progress to cancer. One of the key issues in HSIL natural history studies is defining what “high-grade” lesions mean clinically, and which high-grade lesions are at highest risk of persistence. It is reasonable to assume that those lesions that persist over 3 years of follow-up are those that are at highest risk of progression to cancer. As most HSIL will never progress to anal cancer , identifying risk profiles and correlates of persistence among disease categories is the first step in establishing a better surrogate endpoint for anal cancer risk. This is crucial to ensure that only those at highest risk of progressing to anal cancer receive treatment.
Strengths and limitations of the study
The strength of the SPANC study is that it is one of only a small number of cohort studies globally to perform both cytology and HRA screening on all participants over multiple time-points, as well as performing HPV DNA genotyping. As both anal cytology and HRA are known to under-diagnose HSIL , performing both is critical for the most accurate research-based estimate of the extent of disease. Also, extremely detailed sexual behaviour and other health and lifestyle behaviour data are collected, which will allow excellent characterisation of associations of these factors with anal HPV and related lesions.
Currently, there are no widely accepted guidelines on how anal cancer prevention programs should be implemented. Anal cancer screening only occurs in selected specialised clinics in a small number of countries, not including Australia. As a result, the efficient conduct of SPANC has been hindered by significant infrastructure constraints. Recruitment into the study has been slower than expected, as clinical and laboratory infrastructure has had to be developed by the research team. A shortage of medical personnel trained to perform HRA and the lack of designated clinic space and facilities for these procedures have been major issues.
The SPANC study is a multidisciplinary collaboration, bringing together researchers skilled in epidemiology, behavioural science, nursing, laboratory virology, immunology, sexual health, cytopathology, histopathology, psychology, and health economics. The study results will provide evidence to inform the possible development of guidelines for implementing screening programs in this population. Additionally, the SPANC study is a unique opportunity to address the lack of resources and infrastructure in Australia and to establish an advocacy agenda for additional anal cancer/HSIL diagnostic services for those populations with recognised higher risks of anal cancer.
The SPANC team thanks the participants. The SPANC study is funded by a NHMRC program grant (# 568971) and a Cancer Council NSW Strategic Research Partnership Program grant (#13-11). IMP (#1016307), FJ (#571402) and DJT (#1013353) are supported by Post-doctoral Training Fellowships from the National Health and Medical Research Council. Cytological testing materials are provided by Hologic (Australia) Pty Ltd. The Kirby Institute is affiliated with the Faculty of Medicine, University of New South Wales and funded by the Australian Government of Health and Ageing. The views expressed in this publication do not necessarily represent the position of the Australian Government.
The SPANC study team includes Andrew Grulich, Fengyi Jin, I Mary Poynten, Dave Templeton, Richard Hillman, Kirsten McCaffery, Kirsten Howard, Annabelle Farnsworth, Jennifer Roberts, Suzanne Garland, Sepehr Tabrizi, Kit Fairley, Garrett Prestage, Andrew Carr, Winnie Tong, Carmella Law, Dorothy Machalek, Brian Acraman, Leonie Crampton, Susan Carroll, Alyssa Cornall, Lance Feeney, Eddie Fraissard, Matthew Law, Patrick McGrath, Robert Mellor, Richard Norris, Matthew O’Dwyer, Susan Pendlebury, Kathy Petoumenos, Samuel Phillips, Adele Richards, Lance Schema, Daniel Seeds, Eva Segelov, Julia Thurloe, Rick Varma.
- De Vuyst H, Clifford GM, Nascimento MC, Madeleine MM, Franceschi S: Prevalence and type distribution of human papillomavirus in carcinoma and intraepithelial neoplasia of the vulva, vagina and anus: a meta-analysis. Int J Cancer. 2009, 124 (7): 1626-1636.View ArticlePubMedGoogle Scholar
- Grulich AE, Poynten IM, Machalek DA, Jin F, Templeton DJ, Hillman RJ: The epidemiology of anal cancer. Sex Health. 2012, 9 (6): 504-508.View ArticlePubMedGoogle Scholar
- Edgren G, Sparen P: Risk of anogenital cancer after diagnosis of cervical intraepithelial neoplasia: a prospective population-based study. Lancet Oncol. 2007, 8 (4): 311-316.View ArticlePubMedGoogle Scholar
- Grulich AE, van Leeuwen MT, Falster MO, Vajdic CM: Incidence of cancers in people with HIV/AIDS compared with immunosuppressed transplant recipients: a meta-analysis. Lancet. 2007, 370 (9581): 59-67.View ArticlePubMedGoogle Scholar
- Daling JR, Weiss NS, Hislop TG, Maden C, Coates RJ, Sherman KJ, Ashley RL, Beagrie M, Ryan JA, Corey L: Sexual practices, sexually transmitted diseases, and the incidence of anal cancer. N Engl J Med. 1987, 317 (16): 973-977.View ArticlePubMedGoogle Scholar
- Daling JR, Madeleine MM, Johnson LG, Schwartz SM, Shera KA, Wurscher MA, Carter JJ, Porter PL, Galloway DA, McDougall JK: Human papillomavirus, smoking, and sexual practices in the etiology of anal cancer. Cancer. 2004, 101 (2): 270-280.View ArticlePubMedGoogle Scholar
- Silverberg MJ, Lau B, Justice AC, Engels E, Gill MJ, Goedert JJ, Kirk GD, D'Souza G, Bosch RJ, Brooks JT, et al: Risk of anal cancer in HIV-infected and HIV-uninfected individuals in North America. Clin Infect Dis. 2012, 54 (7): 1026-1034.View ArticlePubMedPubMed CentralGoogle Scholar
- Ault KA: Effect of prophylactic human papillomavirus L1 virus-like-particle vaccine on risk of cervical intraepithelial neoplasia grade 2, grade 3, and adenocarcinoma in situ: a combined analysis of four randomised clinical trials. Lancet. 2007, 369 (9576): 1861-1868.View ArticlePubMedGoogle Scholar
- Palefsky JM, Giuliano AR, Goldstone S, Moreira ED, Aranda C, Jessen H, Hillman R, Ferris D, Coutlee F, Stoler MH, et al: HPV vaccine against anal HPV infection and anal intraepithelial neoplasia. N Engl J Med. 2011, 365 (17): 1576-1585.View ArticlePubMedGoogle Scholar
- Palefsky J, Berry JM, Jay N: Anal cancer screening. Lancet Oncol. 2012, 13 (7): e279-e280. author rreply e280View ArticlePubMedGoogle Scholar
- Macaya A, Munoz-Santos C, Balaguer A, Barbera MJ: Interventions for anal canal intraepithelial neoplasia. Cochrane Database Syst Rev. 2012, 12: CD009244Google Scholar
- Brooks JT, Kaplan JE, Masur H: What's new in the 2009 US guidelines for prevention and treatment of opportunistic infections among adults and adolescents with HIV?. Top HIV Med. 2009, 17 (3): 109-114.PubMedGoogle Scholar
- Machalek DA, Poynten M, Jin F, Fairley CK, Farnsworth A, Garland SM, Hillman RJ, Petoumenos K, Roberts J, Tabrizi SN, et al: Anal human papillomavirus infection and associated neoplastic lesions in men who have sex with men: a systematic review and meta-analysis. Lancet Oncol. 2012, 13 (5): 487-500.View ArticlePubMedGoogle Scholar
- Rogstad KE: The psychological impact of abnormal cytology and colposcopy. BJOG. 2002, 109 (4): 364-368.View ArticlePubMedGoogle Scholar
- Jin F, Prestage GP, Mao L, Kippax SC, Pell CM, Donovan B, Templeton DJ, Taylor J, Mindel A, Kaldor JM, et al: Transmission of herpes simplex virus types 1 and 2 in a prospective cohort of HIV-negative gay men: the health in men study. J Infect Dis. 2006, 194 (5): 561-570.View ArticlePubMedGoogle Scholar
- Prestage G, Mao L, Fogarty A, Van de Ven P, Kippax S, Crawford J, Rawstorne P, Kaldor J, Jin F, Grulich A: How has the sexual behaviour of gay men changed since the onset of AIDS: 1986–2003. Aust N Z J Public Health. 2005, 29 (6): 530-535.View ArticlePubMedGoogle Scholar
- Zablotska IB, Prestage G, Grulich AE, Imrie J: Differing trends in sexual risk behaviours in three Australian states: New South Wales, Victoria and Queensland, 1998–2006. Sex Health. 2008, 5 (2): 125-130.View ArticlePubMedGoogle Scholar
- Zablotska IB, Kippax S, Grulich A, Holt M, Prestage G: Behavioural surveillance among gay men in Australia: methods, findings and policy implications for the prevention of HIV and other sexually transmissible infections. Sex Health. 2011, 8 (3): 272-279.View ArticlePubMedGoogle Scholar
- Zablotska IB, Holt M, Prestage G: Changes in gay men's participation in gay community life: implications for HIV surveillance and research. AIDS Behav. 2012, 16 (3): 669-675.View ArticlePubMedGoogle Scholar
- Banks E, Redman S, Jorm L, Armstrong B, Bauman A, Beard J, Beral V, Byles J, Corbett S, Cumming R, et al: Cohort profile: the 45 and up study. Int J Epidemiol. 2008, 37 (5): 941-947.View ArticlePubMedGoogle Scholar
- Carballo-Dieguez A, Bauermeister JA, Ventuneac A, Dolezal C, Balan I, Remien RH: The use of rectal douches among HIV-uninfected and infected men who have unprotected receptive anal intercourse: implications for rectal microbicides. AIDS Behav. 2008, 12 (6): 860-866.View ArticlePubMedGoogle Scholar
- Roth AJ, Kornblith AB, Batel-Copel L, Peabody E, Scher HI, Holland JC: Rapid screening for psychologic distress in men with prostate carcinoma: a pilot study. Cancer. 1998, 82 (10): 1904-1908.View ArticlePubMedGoogle Scholar
- Shinn E, Basen-Engquist K, Le T, Hansis-Diarte A, Bostic D, Martinez-Cross J, Santos A, Follen M: Distress after an abnormal Pap smear result: scale development and psychometric validation. Prev Med. 2004, 39 (2): 404-412.View ArticlePubMedGoogle Scholar
- Wardle J, Pernet A, Stephens D: Psychological consequences of positive results in cervical cancer screening. Psychol Health. 1995, 10 (3): 185-194.View ArticleGoogle Scholar
- Horowitz M, Wilner N, Alvarez W: Impact of event scale: a measure of subjective stress. Psychosom Med. 1979, 41 (3): 209-218.View ArticlePubMedGoogle Scholar
- Tabrizi SN, Stevens MP, van Leeuwen MT, Vajdic CM, Botes LP, Medley G, Hillman R, Grulich A, Garland SM: Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR. J Clin Microbiol. 2009, 47 (1): 227-229.View ArticlePubMedGoogle Scholar
- Palefsky JM: Practising high-resolution anoscopy. Sex Health. 2012, 9 (6): 580-586.View ArticlePubMedGoogle Scholar
- Jay N, Berry JM, Hogeboom CJ, Holly EA, Darragh TM, Palefsky JM: Colposcopic appearance of anal squamous intraepithelial lesions: relationship to histopathology. Dis Colon Rectum. 1997, 40 (8): 919-928.View ArticlePubMedGoogle Scholar
- Tabrizi SN, Stevens M, Chen S, Rudland E, Kornegay JR, Garland SM: Evaluation of a modified reverse line blot assay for detection and typing of human papillomavirus. Am J Clin Pathol. 2005, 123 (6): 896-899.View ArticlePubMedGoogle Scholar
- Stevens MP, Garland SM, Tabrizi SN: Development and validation of a real-time PCR assay specifically detecting human papillomavirus 52 using the Roche LightCycler 480 system. J Virol Methods. 2008, 147 (2): 290-296.View ArticlePubMedGoogle Scholar
- Darragh T, Birdsong G, Luff RD, Davey D: Anal-rectal cytology. The Bethesda system for reporting cervical cytology. Edited by: Solomon DN R. 2004, New York: Springer, 169-175.View ArticleGoogle Scholar
- Darragh TM, Colgan TJ, Cox JT, Heller DS, Henry MR, Luff RD, McCalmont T, Nayar R, Palefsky JM, Stoler MH, et al: The lower anogenital squamous terminology standardization project for HPV-associated lesions: background and consensus recommendations from the College of American Pathologists and the American Society for Colposcopy and Cervical Pathology. J Low Genit Tract Dis. 2012, 16 (3): 205-242.View ArticlePubMedGoogle Scholar
- Darragh TM, Colgan TJ, Cox JT, Heller DS, Henry MR, Luff RD, McCalmont T, Nayar R, Palefsky JM, Stoler MH, et al: The lower anogenital squamous terminology standardization project for hpv-associated lesions: background and consensus recommendations from the College of American Pathologists and the American Society for Colposcopy and Cervical Pathology. Arch Pathol Lab Med. 2012, 136 (10): 1266-1297.View ArticlePubMedGoogle Scholar
- Oh KY-T, Palefsky J: Diseases of the anus. Diagnostic gynecologic and obstetric pathology. Edited by: Crum CP, Lee KR. 2006, Philadelphia: Elsevier Saunders, 199-227.Google Scholar
- Pirog EC, Quint KD, Yantiss RK: P16/CDKN2A and Ki-67 enhance the detection of anal intraepithelial neoplasia and condyloma and correlate with human papillomavirus detection by polymerase chain reaction. Am J Surg Pathol. 2010, 34 (10): 1449-1455.View ArticlePubMedGoogle Scholar
- Darragh TM, Winkler B: Anal cancer and cervical cancer screening: key differences. Cancer Cytopathol. 2011, 119 (1): 5-19.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2458/13/946/prepub
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