Skip to main content
  • Research article
  • Open access
  • Published:

Diet, life-style and cardiovascular morbidity in the rural, free living population of Elafonisos island



There are about 70 small islands in the Aegean and Ionian Sea, of less than 300 Km2 and 5000 inhabitants each, comprising a total population of more than 75,000 individuals with geographical and socioeconomic characteristics of special interest. The objective of the present study was to assess lifestyle characteristics and the state of cardiovascular risk of the population of a small Eastern Mediterranean island, Elafonisos.


PERSEAS (Prospective Evaluation of cardiovascular Risk Surrogates in Elafonisos Area Study) is an ongoing, population-based, longitudinal survey of cardiovascular risk factors, life-style characteristics and related morbidity/mortality performed in a small and relatively isolated island of the Aegean Sea, named Elafonisos. Validated, closed-ended questionnaires for demographic, socio-economic, clinical and lifestyle characteristics were distributed and analyzed. The MedDietScore, a validated Mediterranean diet score was also calculated. In addition, all participants underwent measurement of anthropometric parameters, blood pressure and a full blood panel for glucose and lipids.


The analysis included 596 individuals who represented 74.5% of the target population. The mean age of the population was 49.5 ± 19.6 years and 48.2% were males. Fifty participants (8.4%) had a history of cardiovascular disease (CVD). The rates of reported diabetes, hypertension, and hypercholesterolemia were 7.7%, 30.9% and 30.9% respectively, with screen-detection of each condition accounting for an additional 4.0%, 12.9%, and 23.3% of cases, respectively. Four hundred and seven individuals (68.3%) were overweight or obese, 25% reported being physically inactive and 36.6% were active smokers. The median MedDietScore was 25 [interquartile range: 6, range 12–47] with higher values significantly associated with older age, better education, increased physical activity, absence of history of diabetes and known history of hypercholesterolemia.


Obesity and traditional risk factors for CVD are highly prevalent among the inhabitants of a small Mediterranean island. Adherence to the traditional Mediterranean diet in this population is moderate, while physical activity is low. There seems to be a need for lifestyle modification programs in order to reverse the increasing cardiovascular risk trends in rural isolated areas of the Mediterranean basin.

Peer Review reports


Over the past half century, large, population-based, epidemiologic studies, among which the Framingham Heart Study represents a landmark [1], have established the prognostic significance of several factors such as hypercholesterolemia, arterial hypertension (AH) and diabetes mellitus (DM) in the appearance of cardiovascular morbidity and mortality [2, 3]. Further investigations demonstrated the etiologic association between the aforementioned morbid conditions and the incidence of cardiovascular disease (CVD), in this way recognizing the former as potential therapeutic targets [4, 5]. Despite the undisputed efficacy and safety of several medications aiming to decrease cardiovascular risk, it has been reported that CVD: a) remains the leading cause of mortality, accounting for approximately one third of all deaths worldwide, b) claims more lives than all cancers combined and c) accounts for annual direct and indirect costs in excess of $ 316.6 billion in the U.S.A. alone [6].

Despite the lack of relevant collective consciousness and education, the importance of behavior-modifying measures in improving health and prolonging survival is paramount [7]. The Mediterranean diet represents a typification of a balanced intake of nutrients, vitamins and traces, while its association with improvement of several health indices has been stressed by various studies [810]. The qualitative composition of the diet, however, varies greatly among Mediterranean countries and within regions of the same country [11].

There are in Greece about 70 small islands spread in the Aegean and Ionian seas, of less than 300 km2 and 5000 inhabitants each, representing a total population of about 75,000 individuals, with geographical and socioeconomic characteristics of special interest. Islands may serve as “epidemiologic laboratories” in which different hypotheses can be tested that could otherwise not be readily assessed elsewhere [12]. Greek island inhabitants have been traditionally occupied with agricultural, farming and piscatorial activities and are considered to be populations highly adherent to the Mediterranean diet. The classic Seven Country Study (SCS), which demonstrated the significance of traditional Mediterranean diet in limiting the incidence of coronary heart disease during the 1950’s, included individuals from two big Greek islands (Crete and Corfu) [13]. Nonetheless, no systematic investigation of dietary and life-style habits in the very small islands and their association with the presence of CVD has ever been performed.

PERSEAS (Prospective Evaluation of cardiovascular Risk Surrogates in Elafonisos Area Study) is an ongoing, population-based, longitudinal survey of cardiovascular risk factors, life-style characteristics and related morbidity/mortality in Elafonisos, a small, relatively isolated island of about 20 km2 and about 1000 inhabitants (Fig. 1). In the present analysis we report the main results of the baseline assessment of PERSEAS, performed during 2012 and 2013. More specifically, we aimed to assess a) the prevalence of established CVD; b) the prevalence of traditional risk factors for CVD (diabetes mellitus, hypertension, hypercholesterolemia); c) dietary and lifestyle habits, and d) factors associated with adherence to the traditional Mediterranean diet.

Fig. 1
figure 1

Map of Elafonisos. The island is highlighted orange


Study population

The target population consisted of all permanent, inhabitants of Elafonisos island > 15 years of age during the study period. The analysis included participants who were interviewed and underwent full clinical evaluation. The reference population (total number of the island adult inhabitants) was defined according to the 2011 census of the Greek population by the National Statistics Agency [, last accessed on 09/01/2016]. The interview and clinical examinations were performed by trained personnel during two pre-specified weeks in September 2012 and September 2013 respectively.

Study questionnaires

The basic questionnaire included information on a wide variety of features including demographics, educational level, smoking habits, sleep habits and a detailed medical history.

Dietary habits were assessed using a validated food-frequency questionnaire. The questionnaire was validated regarding its test-retest reliability in our population with a random sub-group of 50 individuals who completed the questionnaire twice with a two week interval. Pearson’s correlation coefficient was 0.88 (P < 0.001). The individuals answered questions about their typical weekly consumption of different food categories (whole grains, fruits, vegetables, potatoes, legumes, fish, olive oil, red meat, poultry and full fat dairy products). Weekly alcohol consumption was estimated on the basis of 100 ml-wine glass equivalents [14]. A validated dietary score assessing adherence to the traditional Mediterranean diet, the MedDietScore [14], which has been previously described in detail [15, 16], was calculated. In brief, the score takes values between 0 and 55 with greater values indicating higher adherence. The exact scoring according to the frequency of consumption of every food category is depicted in in Table 1 of the Additional file 1.

The level of physical activity was assessed by answering to closed-ended questions regarding the usual type of activity (walking, jogging, sport), the frequency of activity (none, daily, less or more often than three times per week) and its mean duration.

Anthropometric measurements and blood pressure

All participants underwent height and weight measurements on site. An average of two measurements of height was obtained with a calibrated anthropometer, used by trained personnel. The participants were weighed without shoes, wearing light clothing and, subsequently, the two values were averaged. Body weight was determined to the nearest 100 g using a digital scale. Body mass index (BMI) was calculated by bodyweight in kilograms divided by height in meters squared. The patients were divided into BMI categories (underweight, normal weighted, overweight and obese according to the World Health Organization (WHO) classification [17]. Waist circumference, measured at the midpoint between the costal margin and iliac crest in the mid-axillary line, hip circumference, measured at the tip of the hip bone in men, and at the widest point between the hips and the buttocks in women and neck circumference, measured in the midway of the neck, between mid-cervical spine and mid anterior neck were also assessed using a non-stretchable, plastic, measuring tape and rounded to the nearest 0.1 cm. Body fat was measured using electrical bio-impedance (Body Composition Analyzer SC-330, Tanita). Lean body mass was calculated by subtracting body fat from total body weight.

Blood pressure was measured according to the standards outlined in the relevant 2013 European Society of Hypertension/European Society of Cardiology guidelines on AH [18]. Specifically, blood pressure was measured by use of a validated electronic device (M2 Basic Upper Arm Blood Pressure Monitor, Omron). Hypertension was defined as systolic blood pressure ≥140 mmHg and/or diastolic blood pressure ≥90 mmHg. The diagnosis of hypertension was based on two blood pressure measurements in the sitting position. Patients with elevated values of BP were re-evaluated on a second visit.

Blood measurements

All blood measurements were performed in the morning, after an eight-hour, overnight fast. A validated point-of-care portable analyzer was used to measure plasma lipids and glucose levels through a combination of enzymatic methodology and solid-phase technology (CholestechLDX®Laboratory Procedure, Alere) in a 40 μL blood sample, taken from a fingerstick. A second desktop point-of-care analyzer was used for measuring glycated hemoglobin A1c (HbA1c) through boronate affinity method, in a blood sample of 4 μL, drawn from the same fingerstick (Quo-Lab™ A1c, EKF Diagnostics).

CVD and cardiovascular risk factors

Established cardiovascular disease (history of stroke or coronary artery disease) and cardiovascular (CV) risk factors (DM, hypercholesterolemia and AH) were diagnosed on the basis of patient reporting or/and referred medical history or/and relative medication regimen. Unknown diabetes (screen-detected) was diagnosed according to WHO criteria, based on either a fasting serum glucose value equal or higher to 7.0 mmol/l (126 mg/dl) or an HbA1c value equal or higher to 6.5% [19, 20]. The cut-off values for diagnosing pre-diabetes were based on WHO criteria as well, i.e., fasting serum glucose between 5.6 and 6.9 mmol/l (100–125 mg/dl) or/and HbA1c between 5.7 and 6.4%. Unknown AH (screen-detected) was diagnosed when at least two measurements of systolic or/and diastolic arterial pressure were equal or higher to 140 mmHg and/or 90 mmHg respectively. Additionally, unknown hypercholesterolemia was considered when serum values of total cholesterol were equal or higher to 5.2 mmol/l (200 mg/dl). Finally, metabolic syndrome was diagnosed based on the National Cholesterol Education Program Adult Treatment Panel III (2001) definition [21].

Statistical analysis

We used PASW Statistics 19.0 (SPSS, Inc., Chicago, Illinois) for all statistical analyses. Continuous variables are expressed as mean ± standard deviation or median [interquartile range] and categorical variables are expressed as counts and percentages. In order to investigate for associations between adherence to the Mediterranean diet and other variables, linear regression analysis (both univariable and multivariable) was used, with the MedDietScore as the dependent variable. The variables included in the multivariable analysis were those found to be significant in the univariable model, as well as variables with known/putative association with dietary habits based on the literature. The level of statistical significance for inclusion was set at a p value of 0.05 or less. A stepwise backward elimination procedure was used in order to include the significant variables in the multivariable model. In order to test for differences in the frequency of food item consumption between groups, Pearson’s chi-square test was used. All significance tests were 2-tailed, and p <0.05 was considered to be statistically significant.


Demographic and anthropometric characteristics

The initial study population included 612 individuals (76.5% of the island’s target population), who agreed to participate and signed an informed consent. Out of the initial population, 596 individuals (97.4%) provided fully completed questionnaires and underwent full clinical and laboratory assessment and, hence, are reported in the present analysis. The basic demographic and anthropometric characteristics are shown in Table 1. There were 287 males (48.2%) and the mean age of the population was 49.5 ± 19.6 years, with 164 individuals (27.5%) being older than 65 years old. The body mass index of the study participants, stratified by age and sex is shown in Table 2. Eighty five out of 287 men (29.6%) and 117/309 women (37.8%) were obese (BMI ≥ 30Kg/m2), while 26.8% of men and 29.7% of women had a BMI within the normal range (18.5–25.0 Kg/m2).

Table 1 Demographic and anthropometric characteristics of the study population, stratified by sex
Table 2 Counts and percentages of body mass index groups stratified by age and sexa

History of CVD and CVD risk factors

A history of coronary artery disease (CAD) or stroke was reported by 6.4 and 2.0% of the participants, respectively. The prevalence of CVD risk factors (AH, DM and hypercholesterolemia), both known and screen detected, is depicted in Table 3.

Table 3 Prevalence of reported and screen detected classic cardiovascular risk factors stratified by sexa

One hundred and eighty four individuals (30.9%) reported a history of AH, among whom one hundred and seventy one (93%) received anti-hypertensive medications. The most common regimen was the combination of angiotensin II receptor blocker (ARB) and a diuretic (21.4%), followed by an ARB (9.8%), a b-blocker (8.1%) and an angiotensin converting enzyme inhibitor monotherapy (8.2%). Fifty three (30.6%) patients received a calcium channel blocker, but this was rarely used as monotherapy (1.7%). Among those who did not report a history of hypertension, 77 participants (12.9% of the whole population) had screen-detected hypertension, raising the total prevalence to 43.8%.

Forty six individuals (7.7%) reported a history of DM, among whom 37 (80.4%) received a glucose-lowering medication. The most frequently administered drug regimens were metformin alone (43.2%) or in combination with a dipeptidyl peptidase 4 (DPP4) inhibitor (24.3%), while 10.8% received insulin therapy. An additional 24 individuals (4.0% of the total population) were diagnosed with diabetes during the study screening, raising the total prevalence to 11.7%. Pre-diabetes was detected in 139 participants (23.3%), among whom 110 were diagnosed according to fasting plasma glucose and 16 according to HbA1c levels and 13 by both methods.

One hundred and eighty four participants (30.9%) reported a history of hypercholesterolemia with 143 receiving lipid lowering therapy; statin alone (77.6%) or combined with ezetimibe (16.1%) were the most commonly used regimens. An additional 139 individuals had abnormally high levels of total cholesterol, based on the measurements during the study screening, raising the total prevalence of hypercholesterolemia to 54.2%. The rates of reported and screen-detected CVD risk factors stratified by sex and BMI are presented in Table 4.

Table 4 Prevalence of reported and screen detected classic cardiovascular risk factors stratified by body mass index and sexa

Regarding metabolic syndrome, its diagnosis was set in 208 (34.9%) individuals; 102 (35.5%) men and 106 (34.3%) women. The prevalence of metabolic syndrome was 121/444 (27.3%) in individuals ≤ 65 years old and 87/152 (57.2%) in individuals >65 years old.

Lifestyle and dietary habits

Two hundred and eighteen individuals (36.6%) were active smokers with a mean smoking burden of a median 18 [27.5] pack-years, whereas 90 (15.1%) were former smokers with a median time since cessation of 15 [20] years. The mean duration of sleep during the night was 7.5 ± 1.9 h, while the proportion of individuals who slept systematically during mid-day was 57.2%. With regards to physical activity, 72.5% of the individuals reported some kind of regular physical activity during the week, while 27.5% reported that they are physically inactive. Walking was the most commonly reported type of activity (73.0% of cases).

The frequency at which the various items of the dietary questionnaire were consumed by the study participants are depicted in Table 2 of the Additional file 1. Olive oil was consumed on a daily basis by virtually all individuals (96.2%). Fruits and vegetables were consumed on a daily basis by 34.7% and 35.9% respectively, while fish was consumed at least three times per week by 46.3%. Two hundred and seventy participants (45.3%) consumed alcohol on a regular basis, with a median of 2 [3.5] glasses per day, respectively. The mean and the median value of the MedDietScore were 25.3 ± 4.4 and 25 [6] respectively. The distribution of MedDietScore values in the study sample is shown in Fig. 2.

Fig. 2
figure 2

Distribution of MedDietScore values among the study participants

In a further analysis of between sex differences in dietary habits with the use of Chi-square test, men demonstrated less frequent (P = 0.038) consumption of vegetables and full-fat dairy products (P = 0.012), but more frequent consumption of red meat (P = 0.034), fish (P = 0.001) and alcohol (P < 0.001).

Results of the univariable and multivariable analysis examining factors associated with higher adherence to the Mediterranean diet are shown in Table 5. The variables that were shown to be significant in the univariate analysis and were included in the backward step multivariate linear regression test were age, sex, mid-day sleep, presence of physical activity, history of hypertension, hypercholesterolemia and coronary artery disease, waist circumference, arterial blood pressure and heart rate. Furthermore, history of diabetes mellitus and level of education, which are factors known to affect quality of diet were also included in the multivariate analysis. Hence, at the end, age (β:0.067; 95% confidence interval [CI]: 0.044-0.091, P < 0.001), male sex (β:2.199; 95% CI: 1.488-2.909, P < 0.001), history of hypercholesterolemia (β: 0.906; 95% CI: 0.026-1.787, P = 0.044), history of diabetes (β: −1.431; 95% CI: −2.772-[−0.091], P = 0.036), physical activity (any vs. no activity) (β: 1.307; 95% CI: 0.517-2.097, P = 0.001) and level of education (β: 0.407; 95% CI: 0.099-0.715, P = 0.010) were the factors independently associated with a higher dietary score and consequently a better adherence to the Mediterranean diet patterns.

Table 5 Univariate and multivariate linear regression for participants’ baseline characteristics and study measurements as determinants of dietary score


The current study demonstrates that obesity, diabetes, hypertension and hypercholesterolemia are highly prevalent among the inhabitants of the small, relatively isolated Greek island of the Aegean Sea, Elafonisos. Moreover, a large proportion of the participants were unaware of their condition.

The findings of our study are in line with epidemiologic studies repeatedly showing a steep increase of obesity and CVD risk factors worldwide during the last 40 years [22, 23]. Moreover, the prevalence of overweight and obesity in our study approximates the figures reported by a large cross-sectional nationwide survey almost a decade ago [24]. As expected the prevalence of overweight and obesity increases significantly after the age of 45 years in both sex groups. However, in contrast to the previously reported Greek data, obesity seems to be more common among women inhabitants of Elafonisos. This distinct finding could be attributed to the special occupational characteristics of this population, as many of the male inhabitants of the island are engaged in agricultural, farming and piscatorial activities, whereas many of the female inhabitants are mainly occupied in housework activities.

The prevalence of reported DM in our study (7.7%) was comparable to the one of drug-prescribed DM among Greek people > 15 years of age (8.2%), found in a recent, nationwide prescription database study [25]. However, it was significantly lower than the one reported (12.0%) in a recent cross-sectional survey of a randomly selected population of the municipality of Saronikos [26], which is part of the greater Athens area, These differences among individual populations may reflect the effect of genetic, environmental or health policy factors. Finally, with regards to arterial hypertension, our results are in line with those reported by the ATTICA study in 2001 [27]. The prevalence of AH seems to be rapidly increasing among the Greek population and its prevalence is consistently higher in men than women [27].

Observational studies from as early as the 1990’s have outlined a subtle, though apparent overtime trend towards an unhealthier lifestyle adaptation among the Greek population. In a study performed in 1995, Voukiklaris et al. reported increased prevalence of CVD risk factors, including hypertension, dyslipidemia and smoking, accompanied by a respective decrease in heavy physical activity and an impressively higher incidence of CVD in middle-aged men from rural areas of the island of Crete [28]. Later studies from the same geographical area reported increased prevalence of overweight and obesity among farmers; mean BMI was an impressive 7 kg/m2 higher when compared to the cohort of the SCS from 40 years earlier [29]. This change was discussed by the authors as being attributable to the gradual establishment of a sedentary life-style, away from high-intensity physical activity and the adoption of unfavorable dietary habits (increased intake of meat and saturated fat, and decrease in fruit consumption) [30, 31], despite the fact that the caloric intake surprisingly decreased overtime [32, 33]. The island of Crete has been in the spotlight of several epidemiologic studies due to the fact that it provided the initial cohort of the SCS, based on which the concept of the “Mediterranean diet” was perceived and established. On the other hand, Crete (the 5th largest island of the Mediterranean Sea and the largest island in Greece, having approximately 620,000 permanent inhabitants in 2011), includes large urban centers with several daily connections by airplane and ship to the mainland. Consequently, its epidemiological and geographical characteristics are in no case representative of the population of the Greek islands in general.

In the present study population, although the participants reported some dietary habits representative of the traditional Mediterranean diet, such as the everyday consumption of olive oil or the frequent consumption of fish, they also seem to have adopted several aspects of “Westernized” diet, such as the limited consumption of fruits and vegetables and the frequent consumption of meat and sweets. Overall and according to their median MedDietscore (=25), their habits may be characterized as moderately adherent to the Mediterranean diet. Importantly, a strong, independent association was designated between age and adherence to Mediterranean diet in our population. This finding can be potentially attributed to the changes in lifestyle and occupation which have been observed during the past two decades due to the touristic development of the island. In addition, the island population demonstrated a rather sedentary life-style, with complete lack of physical activity in approximately one fourth of the participants, while only 49% reported some form of physical activity on a daily basis. Interestingly, the participants who exercised reported a more healthy dietary behavior, as physical activity along with age, male sex, level of education, history of hypercholesterolemia and lack of history of diabetes were independently associated with higher adherence to the Mediterranean diet. The association between age and healthier dietary habits has been further corroborated by the findings of another study that highlights age as an independent predictor of adherence to Mediterranean diet in a large cohort of individuals from four randomly selected Greek islands [34]. This could be explained not only by the lower caloric requirements of the elderly population, but, additionally, by their increased resistance in adopting newly introduced “Westernized” dietary and life-style trends. Moreover, one must bear in mind that the greater adherence of the elderly to Christian Orthodox rituals, among which is frequent fasting, may in part explain their healthier eating habits, as previously discussed [3537].

The association between history of hypercholesterolemia and higher adherence to the Mediterranean diet comes as no surprise, since dietary recommendations against this condition are compatible to the Mediterranean diet patterns, namely increasing intake of vegetables, fruits, and whole grains, as well as reduced intake of sweets, sugar-sweetened beverages and red meat. On the other hand, since the same dietary recommendations also apply for patients with diabetes, the inverse relationship depicted between history of diabetes and adherence to the Mediterranean diet in our population could possibly indicate either the limited access of the Elafonisos inhabitants to specialized, in the field, physicians or lack of adequate compliance on their behalf. The association between male sex and better adherence to the Mediterranean diet is contradictory to previous results [16, 36]. However, in the present study, this sex difference was driven by an increased intake of alcohol and fish among men, possibly indicating the special social and occupational characteristics of the study’s population. Furthermore, the positive relationship between level of education and adherence to Mediterranean diet comes as no surprise. Education, as a component of higher socioeconomic status, has been shown to correlate inversely with the incidence of CVD. The same findings were confirmed in a recent sub-analysis of the ATTICA population, which showed a significantly higher 10-year incidence of CVD among individuals with lower (≤9 years) vs higher education (≥15 years) [38]. This positive effect can in part be attributed to the greater ease of access to edifying and behavior-modifying information and programs that education ensures. Additionally, higher education is in general paralleled by higher income; this enables better access to higher quality health facilities.

The notably high prevalence of obesity and both known and screen-detected CVD risk factors places the study population at high risk for incident CVD. The relatively low prevalence of reported CVD (8.4%) among this population merits further investigation, as it could reflect either genetic factors or possible beneficial effects of certain aspects of the Mediterranean diet, such as olive oil and fish consumption or a misinterpretation due to the latent period of CVD. Increased inbreeding, which is frequent among isolated populations, reinforces the possibility that genetic factors may play a pivotal role in the disequilibrium between prevalence of CVD and risk factors in our study population. However, similar trends have been reported in the past among the Greek population. In the ATTICA study, the baseline prevalence of known CVD was also low compared to the widely prevalent CVD risk factors [27]. However, CVD incidence during the 5- and 10-year follow-up was as high as 8.5 and 15.7%, respectively [39], raising the total burden of the disease very fast. Consequently, only prospective evaluation during long-term follow-up will help to elucidate the association between risk factors and CVD in this particular population.

Study limitations

Due to the cross-sectional nature of this analysis and the single island population included, generalization of the results should be applied with caution before confirmation is available from larger population analyses. However, this does not diminish the significance of our findings, since they clearly show that the inhabitants of a small island of the Mediterranean basin, have adopted “Westernized” lifestyle habits. Although, the cross-sectional analysis does not allow deduction of causal relationships between the study variables, the fact that overweight, obesity and CVD risk factors are highly prevalent among this population cannot be considered a coincidence.

Although other measures of body composition assessment, such as CT scan and MRI are more accurate than bio-impendance, the latter, was used in due to its simplicity, quickness, low cost and non-invasive nature. Bio-impedance can accurately measure body fat, as far as it is used for specific ethnic groups, populations and conditions, which was actually the case in the present study.

Finally, although a single measurement of fasting serum glucose for the diagnosis of diabetes is subject to error, in epidemiological studies, one measurement is most often used, due to infeasibility of double measurements. In the present study, individuals with high blood glucose results (in the range of diabetes) were advised to show them to their family physicians in order to receive further instructions.


In summary, the prevalence of obesity and classical CVD risk factors is high among the inhabitants of a small Eastern Mediterranean island, showing that even isolated rural populations have adapted many aspect of the “Western” lifestyle. This increase, combined with distancing of the population from traditional dietary and life-style habits should ring a bell for all health policy makers; organized, multi-faceted efforts should be made to promote re-adoption of the Mediterranean diet in an attempt to reduce the burden of CVD and improve population’s health.



Arterial hypertension


Angiotensin II receptor blocker


Body mass index


Coronary artery disease


Confidence interval


Cardiovascular disease


Diabetes mellitus


Dipeptidyl peptidase-4


Glycated hemoglobin A1c


Prospective Evaluation of cardiovascular Risk Surrogates in Elafonisos Area Study


Seven Country Study


  1. Mahmood SS, Levy D, Vasan RS, et al. The Framingham Heart Study and the epidemiology of cardiovascular disease: a historical perspective. Lancet. 2014;383:999–1008.

    Article  PubMed  Google Scholar 

  2. Wilson PW, D’Agostino RB, Levy D, et al. Prediction of coronary heart disease using risk factor categories. Circulation. 1998;97:1837–47.

    Article  CAS  PubMed  Google Scholar 

  3. Laakso M. Diabetes and cardiovascular disease in type 2 diabetes: challenge for treatment and prevention. J Intern Med. 2001;249:225–35.

    Article  CAS  PubMed  Google Scholar 

  4. Haffner SM, Lehto S, Rönnemaa T, et al. Mortality from coronaryheart disease in subjects with type 2 diabetes and in nondiabetic subjects with and without prior myocardial infarction. N Engl J Med. 1998;339:229–34.

    Article  CAS  PubMed  Google Scholar 

  5. Wilkins JT, Ning H, Berry J, et al. Lifetime risk and years lived free of total cardiovascular disease. JAMA. 2012;308:1795–801.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Mozaffarian D, Benjamin EJ, Go AS, et al. Heart disease and stroke statistics-2016 update: a report from the American Heart Association. Circulation. 2016;133:e38–360.

    Article  PubMed  Google Scholar 

  7. Buttar HS, Li T, Ravi N. Prevention of cardiovascular diseases: role of exercise, dietary interventions, obesity and smoking cessation. Exp Clin Cardiol. 2005;10:229–49.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Mitrou PN, Kipnis V, Thiébaut AC, et al. Mediterranean dietary pattern and prediction of all-cause mortality in a US population: results from the NIH-AARP Diet and Health Study. Arch Intern Med. 2007;167:2461–8.

    Article  PubMed  Google Scholar 

  9. Trichopoulou A, Costacou T, Bamia C, et al. Adherence to a Mediterranean diet and survival in a Greek population. N Engl J Med. 2003;348:2599–608.

    Article  PubMed  Google Scholar 

  10. Estruch R, Ros E, Salas-Salvadó J, PREDIMED Study Investigators, et al. Primary prevention of cardiovascular disease with a Mediterranean diet. N Engl J Med. 2013;368:1279–90.

    Article  CAS  PubMed  Google Scholar 

  11. Thanopoulou A, Karamanos B, Angelico F, Multi-Centre Study of the Mediterranean Group for the Study of Diabetes (MGSD), et al. Nutritional habits of subjects with Type 2 diabetes mellitus in the Mediterranean Basin: comparison with the non-diabetic population and the dietary recommendations. Multi-Centre Study of the Mediterranean Group for the Study of Diabetes (MGSD). Diabetologia. 2004;47:367–76.

    Article  CAS  PubMed  Google Scholar 

  12. Cliff AD, Haggett P, Smallman-Raynor MR. Island Epidemics. Oxford: Oxford University Press; 2000.

    Google Scholar 

  13. Verschuren WM, Jacobs DR, Bloemberg BP, et al. Serum total cholesterol and long-term coronary heart disease mortality in different cultures. Twenty-five-year follow-up of the seven countries study. JAMA. 1995;274:131–6.

    Article  CAS  PubMed  Google Scholar 

  14. Panagiotakos DB, Pitsavos C, Arvaniti F, Stefanadis C. Adherence to the Mediterranean food pattern predicts the prevalence of hypertension, hypercholesterolemia, diabetes and obesity, among healthy adults; the accuracy of the MedDietScore. Prev Med. 2007;44(4):335–40.

    Article  PubMed  Google Scholar 

  15. Panagiotakos D, Kalogeropoulos N, Pitsavos C, Roussinou G, Palliou K, Chrysohoou C, Stefanadis C, Panagiotakos D, Kalogeropoulos N, Pitsavos C, Roussinou G, Palliou K, Chrysohoou C, Stefanadis C. Validation of the MedDietScore via the determination of plasma fatty acids. Int J Food Sci Nutr. 2009;60 Suppl 5:168–80.

    Article  CAS  PubMed  Google Scholar 

  16. Panagiotakos DB, Georgousopoulou EN, Pitsavos C, ATTICA Study Group, et al. Exploring the path of Mediterranean diet on 10-year incidence of cardiovascular disease: the ATTICA study 2002–2012. Nutr Metab Cardiovasc Dis. 2015;25:327–35.

    Article  CAS  PubMed  Google Scholar 

  17. Available online at: Accessed 25 Jan 2017.

  18. Mancia G, Fagard R, Narkiewicz K, Task Force Members, et al. 2013 ESH/ESC Guidelines for the management of arterial hypertension: the Task Force for the management of arterial hypertension of the European Society of Hypertension (ESH) and of the European Society of Cardiology (ESC). J Hypertens. 2013;31:1281–357.

    Article  CAS  PubMed  Google Scholar 

  19. Available online at: Accessed 25 Jan 2017.

  20. Available online at: Accessed 25 Jan 2017.

  21. Huang PL. A comprehensive definition for metabolic syndrome. Dis Model Mech. 2009;2(5–6):231–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. NCD Risk Factor Collaboration (NCD-RisC). Worldwide trends in diabetes since 1980: a pooled analysis of 751 population-based studies with 4·4 million participants. Lancet. 2016;387:1513–30.

    Article  Google Scholar 

  23. NCD Risk Factor Collaboration (NCD-RisC). Trends in adult body-mass index in 200 countries from 1975 to 2014: a pooled analysis of 1698 population-based measurement studies with 19·2 million participants. Lancet. 2016;387:1377–96.

    Article  Google Scholar 

  24. Kapantais E, Tzotzas T, Ioannidis I, et al. First national epidemiological survey on the prevalence of obesity and abdominal fat distribution in Greek adults. Ann Nutr Metab. 2006;50:330–8.

    Article  CAS  PubMed  Google Scholar 

  25. Liatis S, Dafoulas GE, Kani C, Politi A, Litsa P, Sfikakis PP, Makrilakis K. The prevalence and treatment patterns of diabetes in the Greek population based on real-world data from the nation-wide prescription database. Diabetes Res Clin Pract. 2016;118:162–7.

    Article  PubMed  Google Scholar 

  26. Gikas A, Lambadiari V, Sotiropoulos A, Panagiotakos D, Pappas S. Prevalence of major cardiovascular risk factors and coronary heart disease in a sample of Greek adults: the saronikos study. Open Cardiovasc Med J. 2016;10:69–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Panagiotakos DB, Pitsavos C, Chrysohoou C, et al. Prevalence and five-year incidence (2001–2006) of cardiovascular disease risk factors in a Greek sample: the ATTICA study. Hellenic J Cardiol. 2009;50:388–95.

    PubMed  Google Scholar 

  28. Voukiklaris GE, Kafatos A, Dontas AS. Changing prevalence of coronary heart disease risk factors and cardiovascular diseases in men of a rural area of Crete from 1960 to 1991. Angiology. 1996;47:43–9.

    Article  CAS  PubMed  Google Scholar 

  29. Vardavas CI, Linardakis MK, Hatzis CM, et al. Prevalence of obesity and physical inactivity among farmers from Crete (Greece), four decades after the Seven Countries Study. Nutr Metab Cardiovasc Dis. 2009;19:156–62.

    Article  CAS  PubMed  Google Scholar 

  30. Kromhout D, Menotti A, Blackburn H (eds). Prevention of Coronary Heart Disease: Diet, Lifestyle and Risk Factors in the Seven Countries Study. Kluwer Academic Publishers; 2002.

  31. Vardavas CI, Linardakis MK, Hatzis CM, et al. Cardiovascular disease risk factors and dietary habits of farmers from Crete 45 years after the first description of the Mediterranean diet. Eur J Cardiovasc Prev Rehabil. 2010;17:440–6.

    Article  PubMed  Google Scholar 

  32. Vardavas CI, Linardakis M, Hatzis C, et al. Smoking status in relation to serum and dietary vitamin and micronutrient concentrations. Tob Induc Dis. 2008;4:8.

    Article  PubMed  PubMed Central  Google Scholar 

  33. Kromhout D, Keys A, Aravanis C, et al. Food consumption patterns in the 1960’s in seven countries. Am J Clin Nutr. 1989;49:889e94.

    Google Scholar 

  34. Romaguera D, Bamia C, Pons A, et al. Food patterns and Mediterranean diet in western and eastern Mediterranean islands. Public Health Nutr. 2009;12:1174–81.

    Article  PubMed  Google Scholar 

  35. Sarri KO, Tzanakis NE, Linardakis MK, et al. Effects of Greek Orthodox Christian Church fasting on serum lipids and obesity. BMC Public Health. 2003;3:16.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Sarri KO, Linardakis MK, Bervanaki FN, et al. Greek Orthodox fasting rituals: a hidden characteristic of the Mediterranean diet of Crete. Br J Nutr. 2004;92:277–84.

    Article  CAS  PubMed  Google Scholar 

  37. Sarri K, Kafatos A. The seven countries study in Crete: olive oil, Mediterranean diet or fasting? Public Health Nutr. 2005;8:666.

    Article  PubMed  Google Scholar 

  38. Kollia N, Panagiotakos DB, Georgousopoulou E, Chrysohoou C, Tousoulis D, Stefanadis C, Papageorgiou C, Pitsavos C. Exploring the association between low socioeconomic status and cardiovascular disease risk in healthy Greeks, in the years of financial crisis (2002–2012): The ATTICA study. Int J Cardiol. 2016;223:758–63.

    Article  PubMed  Google Scholar 

  39. Kastorini CM, Panagiotakos DB, Georgousopoulou EN, ATTICA Study Group, et al. Metabolic syndrome and 10-year cardiovascular disease incidence: The ATTICA study. Nutr Metab Cardiovasc Dis. 2016;26:223–31.

    Article  PubMed  Google Scholar 

Download references


The authors would like to thank the PERSEAS study group of investigators: Emmanouil Mpelliotis, Ioannis Papadopoulos, Filitsa Spanoudi, Konstantinos Sgouros, Dimitris Logothetis, Eirini Papaneofytou, Gerasimos Markatos, Stamatia Moschakou, Dimitrios Mpramos, Ekaterini Konstantatou, Georgios Antonakoudis, Matina Nezi and Matina Kleftaki for their assistance in the initial physical examination and George Markozannes for his assistance with the statistical analysis of data. The authors would also like to thank the Medical Association of Lakonia, the Municipality of Elafonisos and the Greek Ministry of Health for their aid in the performance of our study.


This study was financially supported by Mylan Hellas. The funding source did not have any involvement in study design, collection, analysis and interpretation of data, writing of the report and decision to submit the article for publication.

Availability of data and materials

The dataset supporting the conclusions of this article is included within the Additional file 2 of the article.

Authors’ contributions

IK, II, CD, EH and SL conceptualized the project and developed the survey. CJK and SL collected data and conducted statistical analysis. All of the authors contributed to the writing of the manuscript, including approval of the final version.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval and consent to participate

This study was conducted according to the guidelines laid down in the Declaration of Helsinki and all procedures involving human subjects were approved by the National Organization for Medicines which acted as an ethics committee in our study. Written informed consent was obtained from all subjects.

Author information

Authors and Affiliations



Corresponding author

Correspondence to Chris J. Kapelios.

Additional file

Additional file 1:

Table 1. MedDietScore points according to frequency of consumption of different food item categories. Table 2. Frequency of consumption of different food item categories (DOCX 17 kb)

Additional file 2:

PERSEAS population raw data. (XLSX 194 kb)

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kapelios, C.J., Kyriazis, I., Ioannidis, I. et al. Diet, life-style and cardiovascular morbidity in the rural, free living population of Elafonisos island. BMC Public Health 17, 147 (2017).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: