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Viral transmission risk factors in an Egyptian population with high hepatitis C prevalence
© Mohlman et al. 2015
Received: 14 April 2015
Accepted: 29 September 2015
Published: 7 October 2015
Egypt has the world’s highest prevalence of infection with hepatitis C virus (HCV), which is a major cause of hepatocellular carcinoma. The high HCV prevalence is largely attributed to the parenteral antischistosomal therapy (PAT) campaigns conducted from the 1950s through the 1980s; however, the primary modes of transmission in the post-PAT period are not well known. In this study we examined the associations between HCV prevalence and exposures to risk factors, including PAT, in a high HCV prevalence population.
Using a cross-sectional design, we examined the associations between demographic characteristics and risk factors for HCV transmission and HCV positivity prevalence among a sample of Egyptian residents. Data were collected through an interview-administered survey, and the association estimates were determined using χ2 and logistic regression.
The highest HCV positivity prevalence was observed in cohorts born before 1960, and declined precipitously thereafter; whereas the proportion of subjects reporting PAT remained relatively stable. Being male, having a rural residence, and having received PAT were all associated with HCV positivity; however, PAT alone could not account for the high prevalence of HCV.
In Egypt, PAT and other transmission factors yet to be identified, as well as cohorts born before the 1960s and infected with HCV, are most likely the main contributors to the current HCV endemic.
Hepatitis C virus (HCV), a blood-borne pathogen, is a major cause of hepatocellular carcinoma (HCC). The incidence of this cancer is increasing worldwide , particularly in Egypt, which has the world’s highest HCV prevalence. From 1997 to 2001, Egypt’s incidence of liver cancer doubled , and a recent estimate of incidence is 38.1 per 100,000 for males and 14.1 per 100,000 for females . Those chronically infected with HCV are 15 to 20 times more likely to develop HCC than those who are not infected .
In Egypt, the prevalence of the two major biomarkers of HCV - the anti-HCV antibodies and HCV RNA seropositivity - is estimated at 14.7 and 9.8 %, respectively, in the general population; but it is much higher among those over age 50 (>35.0 % for anti-HCV antibodies and > 25 % for HCV RNA) . The source of this epidemic has largely been attributed to the parenteral antischistosomal therapy (PAT) campaigns that took place from the 1950s through the 1980s ; however, the primary modes of transmission in the post-PAT period are not well known. Iatrogenic sources have been considered key contributors, especially among the elderly who are at risk of chronic diseases and thus potential medical treatments. While Egypt has made great strides in improving infection control, an overburdened, under-funded healthcare system does not always promote optimal measures . This especially holds true where an informal healthcare system operates in parallel to the official one . Breban et al., using a dynamic model of HCV transmission, postulated that ongoing HCV transmission is fueled in large part by a small group of infectious individuals with high rates of medical treatments and healthcare provision with suboptimal infection control, resulting in the lasting impact of iatrogenic transmission .
In this study, we examined the associations between demographic characteristics, risk factors for HCV transmission, and HCV seropositivity prevalence. Our aim was to understand how HCV infection among the oldest group in our study sample could contribute to current transmission trends.
Study population and data collection
For the present study we used a cross-sectional analysis to examine the control group that participated in a case–control study on HCC (hereafter referred to as the HCC study) conducted in Egypt between 1999 and 2010. Details of the HCC study enrollment, case confirmation, interview procedures, and participation have been previously described in detail . Briefly, participants were residents of Egypt who lived in the Cairo-Giza metropolitan area and in surrounding governorates. Cases, defined as those diagnosed with HCC, were recruited at the National Cancer Institute of Cairo University. Controls, recruited at Cairo University’s orthopedic hospital, were selected to frequency-match the cases by gender, age category (5-year age groups), and current residence category (i.e. rural or urban). Additional rural male controls were recruited at public health clinics in villages of the Qalyubia Governorate, north of Cairo, to ensure adequate matching by residence .
Inclusion criteria included being over the age of 17 and having been resident in Egypt for at least a year. All participants signed informed consent forms or had a witness sign if they were illiterate. Information on those who refused to participate and their reason was also documented. The protocol was approved by the Institutional Review Boards of Georgetown University, the University of Maryland, Baltimore, and Cairo University. An interviewer administered the questionnaire face-to-face to participants and recorded their answers. Additionally, researchers collected ten milliliters of blood from participants for serological testing of HCV antibodies and HCV RNA, as well as hepatitis B virus (HBV) core antibodies and HBV surface antigen, as previously described .
Between 2001 and through 2010, we recruited 1764 controls for the HCC study. We collected data on demographics and potential environmental risk factors; i.e., tobacco smoking, alcohol drinking, environmental tobacco smoke, pesticides, and water and air pollution . In addition, the HCC study inquired about five risk factors with the potential for exposure to HCV-contaminated blood or instruments: receiving PAT, receiving a blood transfusion, donating blood, receiving therapeutic injections, and a diagnosis of diabetes, which often leads to insulin injections. This study focused on the demographic, PAT, and medical variables with potential for exposure to HCV.
Assessment of HCV status
Detailed descriptions of testing have been previously described [10, 11]. Briefly, ten milliliters of blood were collected from each patient by venipuncture. Blood was separated and the serum divided into three aliquots, one of which was analyzed immediately, while the other two were stored at −80 °C. To identify the presence of HCV antibodies, we used third generation ELISA screening tests (Abbott Laboratories) . A reverse transcription (RT)-PCR based method, developed by Abel-Hamid and colleagues , was used to test for HCV RNA. Where the ELISA test was positive and the RT-PCR test was negative, the latter test was repeated after re-extracting and purifying the RNA from the whole blood specimen . HCV positivity in this study was defined as positive for HCV antibodies and/or HCV RNA.
Data analysis included descriptive statistics of the study sample by age, gender, birthplace, current residence, education, and medical history. Pearson χ2 tests were used to compare HCV status between groups. Logistic regression was used to generate the unadjusted and adjusted odds ratio (OR and AOR) and 95 % confidence interval (CI) of the association between HCV status and each of the above variables. The two-sample proportion test was used to compare HCV status and PAT prevalence. Since the data were collected over a 10-year period, we converted age at interview to year of birth to appropriately apportion the study sample into 10-year age groups. Stata 13 was used for all statistical analysis.
Gender, birthplace, and current residence of the different age groups of a sample of the Egyptian population
Age groups (year of birth)
Birthplace (% rural)
Residence at time of interview (% rural)
Approximately 65.2 % of the participants were born in a rural area, but only 56.5 % lived in rural area at the time of the interview (Table 1), indicating urban migration. Overall, 18.2 % of respondents had not relocated from their birthplace at the time of the interview. Of these, 230 (72.1 %) lived in rural areas. Of those who had moved, 7.1 % moved from an urban to a rural area, and 17.8 % moved from a rural to an urban area.
Risk factors for HCV transmission
Associations between HCV positivity (anti-HCV antibodies and/or HCV RNA positive) and different demographic characteristics among a sample of the Egyptian population
Study population characteristic
HCV Prevalence (%)
Unadjusted OR (95 % CI)
Adjusted OR (95 % CI)a
0.71 (0.56, 0.91)
0.65 (0.48, 0.86)
1.20 (0.79, 1.82)
1.32 (0.85, 2.04)
1.07 (0.73, 1.57)
1.17 (0.78, 1.76)
0.55 (0.36, 0.83)
0.59 (0.38, 0.91)
0.26 (0.16, 0.43)
0.27 (0.16, 0.45)
0.11 (0.06, 0.19)
0.10 (0.06, 0.20)
No schooling, literacy only, or religious school
Primary through secondary
0.69 (0.54, 0.88)
1.21 (0.91, 1.61)
0.55 (0.46, 0.66)
0.90 (0.51, 1.59)
Current same as birth – rural
Current same as birth - urban
0.34 (0.18, 0.66)
0.41 (0.20, 0.84)
Urban to urban migration
0.40 (0.27, 0.58)
0.32 (0.21, 0.50)
Rural to rural migration
1.30 (0.92, 1.82)
1.03 (0.70, 1.51)
Rural to urban migration
0.88 (0.59, 1.3)
0.64 (0.41, 0.99)
Urban to rural migration
0.26 (0.12, 0.57)
0.21 (0.09, 0.48)
3.00 (2.30, 3.91)
2.36 (1.68, 3.31) b
Residence at time of interview
2.04 (1.62, 2.57)
1.35 (1.01, 1.83) b
In the regression model, we found that females were 37 % less likely than males to be HCV positive after adjustment for age, residence (both at birth and interview time), and education. We found no statistically significant difference in HCV positivity between those born before 1940 and those born between 1940 and 1959. For those born after 1959, the odds of being HCV positive decreased gradually with each birth decade (Table 2). Increasing educational attainment lost its statistical significance as an indicator of HCV positivity after adjustment for age, gender, and migration status (Table 2). We also observed that a rural birthplace was associated with being HCV positive (AOR 2.36 (95 % CI 1.68, 3.31)), as was current residence in rural areas (AOR 1.35 (95 % 1.01, 3.31)), although to a lesser extent (Table 2). When we assessed the effects of migration and its patterns, using being born and living in the same rural residence as the reference group, all groups had significantly lower odds of being HCV positive, except the group with a rural-to-rural migration pattern, for which there was no significant difference (Table 2).
The age group with the highest lifetime exposure to transmission risk factors included those born between 1950 and 1959 (22.7 % exposed to PAT; 22.4 % exposed to blood transfusions, 25 % donated blood, 34.2 % had diabetes, and 34.8 % had frequent or regular injections). In this group, the prevalence of HCV positivity was high among those who were exposed to PAT (44.8 %); received blood transfusion (43.5 %); donated blood (40.0 %); were diagnosed with diabetes (46.0); and those who received frequent or regular injections (34.4 %).
In contrast, those born after 1980 had modest to low exposure to risk factors (16 %–17 % were exposed to PAT, blood transfusion, and blood donation; 1.6 % had diabetes; and none had frequent or regular injections). Of those exposed to these risk factors, the prevalence of HCV ranged from 0.0 % (diabetes and frequent or regular injections) to 8.6 % (blood donation). PAT and blood transfusion fell in the middle with 4.9 and 6.7 % prevalence respectively.
Associations between HCV positivity (anti-HCV antibodies and/or HCV RNA positive) and exposures to potential routes of HCV transmission in a sample of the Egyptian population
Route of Transmission
HCV Prevalence, %
Unadjusted OR (95 % CI)
Adjusted OR (95 % CI)a
1.62 (1.22, 2.16)
1.54 (1.14, 2.11)
1.16 (0.87, 1.53)
1.31 (0.96, 1.79)
0.76 (0.59, 0.98)
0.76 (0.57, 1.02)
1.61 (1.16, 2.22)
1.23 (0.87, 1.76)
Number of Injections
Less than 10 injections
10 or more injections
1.35 (0.87, 2.10)
1.24 (0.76, 2.02)
Has injections frequently/continuously
1.45 (0.78, 2.67)
1.23, (0.63, 2.41)
Our findings suggest that in Egypt, 1) PAT and other risk factors yet to be identified were historically and remain important for HCV transmission; and 2) cohorts born before the 1960s with high HCV prevalence are most likely the main contributors to the current HCV endemic. This last point is in line with the proposal put forward by Breban et al. that a small infectious population with high rates of medical exposures is a significant source of transmission in Egypt .
Our study sample characteristics are comparable to those of the nationally representative sample recruited and tested for HCV positivity through the Egypt Demographic and Health Survey (EDHS)  for health policy purposes. Although we found the overall prevalence of anti-HCV antibodies and HCV RNA to be much higher in our study population (29.6 and 22.9 % respectively) than in the EDHS (14.7 and 9.8 %), the prevalence was similar when we examined comparable age groups and gender. Indeed, of those born between 1950 and 1959 in our study population, the prevalence of HCV positivity was 49.5 % for males and 30.9 % for females, compared to 46.3 and 30.8 % respectively in this age group in the EDHS. Furthermore, the EDHS estimated that 53.9 % of males and 54.8 % of females lived in rural areas at the time of the study, proportions that are close to those we found of 58.9 and 52.6 %, respectively. And both our study and the EDHS found higher prevalence of HCV positivity in rural than in urban communities. The discrepancy in the overall prevalence of HCV positivity is most likely due to the EDHS sample having higher proportions of young individuals (testing for HCV was limited to those 15 to 59 years old), who tended to be more educated than the older ones and have lower HCV prevalence. Our sample population had a higher proportion of those who were living in rural areas and of those with less primary schooling. Nonetheless, both our study and the EDHS showed that less education and rural residence were associated with higher HCV positivity prevalence .
We found PAT to be the only risk factor that was significantly associated with HCV positivity; however in comparing the proportion of those who were HCV positive to the proportion of those who had received PAT (Fig. 1), we noted that the prevalence of HCV positive cases in each age group, except the two youngest, was much higher than the proportion of those exposed to PAT. Therefore, PAT cannot fully explain the high HCV prevalence in Egypt; transmission that occurred through additional routes, perhaps iatrogenic and community based, is highly likely . Such a possibility is supported by our findings that the groups with high HCV positivity prevalence also had high prevalence of lifetime exposure to blood transfusion, blood donation, diabetes diagnosis, and frequent or regular injections. Another explanation for the trends in Fig. 1 is that PAT injection practices were riskier in the earlier half of the 20th century but became safer thereafter.
Our study did not find a statistically significant association between HCV positivity and blood transfusion, blood donation, therapeutic injections, and diabetes diagnosis (after adjustment for demographic factors), in contrast to other studies that focused on these risk factors [12, 14–16]. However, the association between aging populations and increased healthcare utilization , which in turn increase the risk for HCV transmission, and the high HCV positivity prevalence among individuals born before 1960 suggest that the latter cohorts may substantially contribute to the ongoing HCV transmission in Egypt; a plausibility that is consistent with Breban et al.’s concept .
One of the study’s limitations was that the main focus of the HCC study was on cancer risk factors; therefore it did not assess all possible HCV transmission risk factors such as the informal healthcare sector, intrafamilial transmission, and exposure risks identified by other researchers [17–20]. The lack of association between some of the known transmission risk factors and HCV positivity, not just in our study but in many others [14, 15, 21, 22], highlights the difficulty in studying an often asymptomatic disease where the time point and source of infection are difficult to identify.
In summary, we found that PAT and aging cohorts of individuals infected with HCV are not the only contributing factor to the current HCV endemic in Egypt, although they play major roles; additional factors of viral transmission are yet to be identified. Therefore, research investigating the relationships of current HCV prevalence rates to historical transmission risk factors among different segments of Egypt’s population are warranted. Temporal changes in iatrogenic and community based modes of transmission may underlie current patterns of prevalence, but remain to be fully elucidated.
This work was supported by the National Institutes of Health [grant number R01CA85888].
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- World Cancer Statistics, World Cancer Research Fund International. http://www.wcrf.org/cancer_statistics/world_cancer_statistics.php.
- Anwar W, Khaled H, Amra H, El-Nezami H, Loffredo CA. Changing pattern of hepatocellular carcinoma (HCC) and its risk factors in Egypt: Possibilities for prevention. Mutat Res. 2008;659:176–84.View ArticlePubMedGoogle Scholar
- Cancer Fact Sheet, International Agency for Research on Cancer. http://globocan.iarc.fr/Pages/fact_sheets_population.aspx.
- El-Serag H. Hepatocellular carcinoma. N Engl J Med. 2011;365:1118–27.View ArticlePubMedGoogle Scholar
- El Zanaty F, Way A. Egypt demographic and health survey 2008, Egypt Ministry of Health. Egypt: Cairo: l-Zanaty and Associates, and Macro International; 2009.Google Scholar
- Frank C, Mohamed MK, Strickland GT, Lavanchy D, Arthur RR, Magder LS, et al. The role of parenteral antischistosomal therapy in the spread of hepatitis C virus in Egypt. Lancet. 2000;355:887–91.View ArticlePubMedGoogle Scholar
- Ismail N, Aboul Ftouh A, El-Shoubary W, Mahaba H. Safe injection practice among health-care workers in Gharbiya Governorate, Egypt. East Mediterr Health J. 2007;13:893–906.PubMedGoogle Scholar
- El Katsha S, Labeeb S, Watts S, Younis A. Informal health providers and the transmission of hepatitis C virus: pilot study in two Egyptian villages. East Mediterr Health J. 2006;12:758–67.PubMedGoogle Scholar
- Breban R, Arafa N, Leroy S, Mostafa A, Bakr I, Tondeur L, et al. Effect of preventative and curative interventions on hepatitis C virus transmission in Egypt (ANRS 1211): a modeling study. Lancet Glob Health. 2014;2:e541–9.View ArticlePubMedGoogle Scholar
- Ezzat S, Abdel-Hamid M, Eissa SA, Mokhtar N, Labib NA, El-Ghorory L, et al. Associations of pesticides, HCV, HBV, and hepatocellular carcinoma in Egypt. Int J Hyg Environ Health. 2005;208:329–39.View ArticlePubMedGoogle Scholar
- Abdel-Hamid M, Edelman DC, Highsmith WE, Constantine NT. Optimization, assessment, and proposed use of direct nested reverse transcription-polymerase chain reaction protocol for the detection of hepatitis C virus. J Hum Virol. 1997;1:58–65.PubMedGoogle Scholar
- Guerra J, Garenne M, Mohamed MK, Fontanet A. HCV burden of infection in Egypt: results from a nationwide survey. J Viral Hepat. 2012;19:560–7.View ArticlePubMedGoogle Scholar
- Miller F, Abu-Raddad L. Evidence of intense ongoing endemic transmission of hepatitis C virus in Egypt. Proc Natl Acad Sci U S A. 2010;107:14757–62.View ArticlePubMedPubMed CentralGoogle Scholar
- Medhat A, Shehata M, Magder LS, Mikhail N, Abdel-Baki L, Nafeh M, et al. Hepatitis C in a community in upper Egypt: risk factors for infection. Am J Trop Med Hyg. 2002;66:633–8.PubMedGoogle Scholar
- Habib M, Mohamed MK, Abdel Aziz F, Magder LS, Abdel Hamid M, Gamil F, et al. Hepatitis C virus infection in a community in the Nile delta: risk factors for seropositivity. Hepatology. 2001;33:248–53.View ArticlePubMedGoogle Scholar
- Arafa N, El Hoseiny M, Rekacewicz C, Bakr I, El-Kafrawy S, El Daly M, et al. Changing pattern of hepatitis C virus spread in rural areas of Egypt. J Hepatol. 2005;43:418–24.View ArticlePubMedGoogle Scholar
- Mohamed MK, Abdel-Hamid M, Mikhail NN, Abdel-Aziz F, Medhat A, Magder LS, et al. Intrafamilial transmission of hepatitis C in Egypt. Hepatology. 2005;42:683–7.View ArticlePubMedGoogle Scholar
- Mohamed MK, Magder LS, Abdel-Hamid M, El-Daly M, Mikhail NN, Abdel-Aziz F, et al. Transmission of hepatitis C virus between parents and children. Am J Trop Med Hyg. 2006;75:16–20.PubMedGoogle Scholar
- Paez Jimenez A, Sharaf Eldin N, Rimlinger F, El-Daly M, El-Hariri H, El-Hoseiny M, et al. HCV iatrogenic and intrafamilial transmission in Greater Cairo, Egypt. Gut. 2010;59:1554–156.View ArticlePubMedGoogle Scholar
- Plancoulaine S, Mohamed MK, Arafa N, Bakr I, Rekacewicz C, Trégouët DA, et al. Dissection of familial correlations in hepatitis C virus (HCV) seroprevalence suggests intrafamilial viral transmission and genetic predisposition to infection. Gut. 2008;57:1268–74.View ArticlePubMedGoogle Scholar
- Stoszek SK, Abdel-Hamid M, Narooz S, El Daly M, Saleh DA, Mikhail N, et al. Prevalence of and risk factors for hepatitis C in rural pregnant Egyptian women. Trans R Soc Trop Med Hyg. 2006;100:102–7.View ArticlePubMedGoogle Scholar
- Mostafa A, Taylor SM, El-Daly M, El Hoseiny M, Bakr I, Arafa N, et al. Is the hepatitis C virus epidemic over in Egypt? Incidence and risk factors of new hepatitis C virus infection. Liver Int. 2010;30:560–6.View ArticlePubMedGoogle Scholar