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Sputum microscopy for the diagnosis of HIV-associated pulmonary tuberculosis in Tanzania
- Mecky Matee†1Email author,
- Lillian Mtei†1,
- Tarja Lounasvaara†2,
- Wendy Wieland-Alter†3,
- Richard Waddell†3,
- Johnson Lyimo†1,
- Muhammad Bakari†1,
- Kisali Pallangyo†1 and
- C Fordham von Reyn†3
© Matee et al; licensee BioMed Central Ltd. 2008
Received: 16 November 2007
Accepted: 21 February 2008
Published: 21 February 2008
In many resource poor settings only sputum microscopy is employed for the diagnosis of HIV-associated pulmonary tuberculosis; sputum culture may not be available.
We determined the diagnostic accuracy of sputum microscopy for active case finding of HIV-associated pulmonary tuberculosis using TB culture as the reference standard.
2216 potential subjects screened for a TB vaccine trial submitted 9454 expectorated sputum specimens: 212 (2.2%) were sputum culture positive for Mycobacterium tuberculosis (MTB), 31 (0.3%) for non-tuberculous mycobacteria, and 79 (0.8%) were contaminated. The overall sensitivity of sputum microscopy was 61.8% (131/212) and specificity 99.7% (9108/9132). Sputum microscopy sensitivity varied from 22.6% in specimens with < 20 colony forming units (CFU)/specimen to 94.2% in patients with > 100 CFU/specimen plus confluent growth. The incremental diagnostic value for sputum microscopy was 92.1%, 1.8% and 7.1% for the first, second and third specimens, respectively. The positive predictive value and negative predictive values for sputum microscopy were 84.5% and 99.1%, respectively. The likelihood ratio (LR) of a positive sputum microscopy was 235.1 (95% CI 155.8 – 354.8), while the LR of a negative test was 0.38 (95CI 0.32 – 0.45). The 212 positive sputum cultures for MTB represented 103 patients; sputum microscopy was positive for 57 (55.3%) of 103 patients.
Sputum microscopy on 3 expectorated sputum specimens will only detect 55% of culture positive HIV-infected patients in active screening for pulmonary tuberculosis. Sensitivity is higher in patients with greater numbers of CFUs in the sputum. Culture is required for active case finding of HIV- associated pulmonary tuberculosis.
Despite recent advances in mycobacteriology , early laboratory diagnosis of tuberculosis in the vast majority of disease-endemic countries remains microscopic examination of the stained sputum smear [2, 3]. The standard WHO recommendation for TB diagnosis in the DOTS program is the use of direct sputum microscopy on 3 stained sputum specimens . First and third are on spot, while the second is the early morning sample. Currently no other diagnostic tool, including sputum culture, is available which could be implemented affordably in resource poor settings, where the burden of disease is greatest.
However, even before the current HIV epidemic it was recognized that the sensitivity of sputum microscopy for the microbiologic confirmation of TB was limited. With HIV infection the sensitivity of sputum microscopy may be reduced even further because of the lower rate of caseation necrosis, and consequent lower numbers of AFB in the airway . HIV may also reduce the specificity of sputum microscopy by increasing the proportion of patients with non-tuberculous mycobacteria . Further, there is still a debate about whether two or three sputum specimens should be examined for diagnosis of TB [7–9]. Reducing the recommended number of specimens examined from three to two could benefit TB control programs by using fewer resources and by reducing the time spent on case detection . In the present study we assessed the test characteristics of smear microscopy on three concentrated sputum specimens during active TB case finding among HIV-infected patients in Dar es Salaam, Tanzania.
Patients and study protocol
Participants were 2216 HIV-positive subjects screened for enrollment in the DARDAR Study between October 2001 and September 2006. DARDAR is a study on the epidemiology and vaccine-based prevention of HIV-associated tuberculosis  being conducted in Dar es Salaam, Tanzania. Eligibility required a CD4 count > 200, a BCG scar, and no evidence of active TB. At baseline, all potential subjects had a physical examination and standardized interview that included questions about weight loss in the past 3 months and about the presence and duration of any cough or fever. All subjects had a baseline chest x-ray and submitted 3 expectorated sputum samples for microbiologic testing. Subjects were instructed in the need for deep cough and asked to provide one spot sputum sample and to label and bring two first morning samples; subjects who were unable to produce a spot specimen were asked to bring three first morning samples. Subjects with active TB on baseline screening were not eligible for further study. Eligible subjects were started on the immunization protocol and were seen every 3 months and whenever new symptoms develop. At each visit subjects were re-evaluated for tuberculosis with physical examination, chest x-ray and collection of 3 expectorated sputum samples.
Sputum samples were decontaminated using the modified Petroff's method and concentrated by centrifugation at 3000 g for 15 minutes. Smears were screened by auramine staining and positive smears were counterstained by the Ziehl Neelsen (ZN) staining technique without removing the auramine. Smears were read without knowledge of culture outcomes and results were categorized as 3+ (> 10 AFB/oil field), 2+ (1 – 10 AFB/oil field), 1+ (10 – 99 AFB/100 oil fields), scanty (1 – 9 AFB/100 oil fields) and negative (0 AFB/100 oil fields). For each smear, a total of 100 microscopic fields were examined as per protocol. Each sample was then cultured in both pyruvate and glycerol containing Lowenstein Jensen media at 37°C for up to 8 weeks. Plates were examined weekly for growth. Colonies were identified according to criteria based on the speed of growth and macroscopic features e.g. roughness and pigment production. Culture results were expressed as actual number of colonies (if less than 20 colonies/slant) 1+ (20–100 colonies/slant, 2+ (discrete innumerable colonies/slant) and 3+ (for confluent growth). Positive cultures were shipped to the US for confirmation by DNA probe (AccuProbe, San Diego, CA, USA).
Quality assurance was accomplished by assessing the quality and adequacy of specimens, and by monitoring microscopy and culture procedures, preparation and storage of reagents and performance of equipment against established laboratory operating procedures. Patients were requested to provide an additional specimen in case of submitting either an inadequate or salivary sample.
For smear microscopy, positive and negative control slides were included with each batch of new reagents and, in a blind manner, when reading patient smears. All slides were read independently by three experienced microscopists, and kept for up to three months for external quality control. Review of smear and culture results provided an internal quality assurance measure. Additional quality measures for cultures included monitoring of; quality of water, decontamination, digestion, and concentration procedures, inspissation and incubation temperatures, and measurement and adjustment of pH of culture media. A standard laboratory strain M. tuberculosis H37Rv was used as a positive control. Identification of mycobacteria was based on growth and colonial morphology and was confirmed by further testing of the isolates using DNA probes (Accuprobe; Gen-Probe).
Between 2001 and 2006 the study laboratory participated in the UK National External Quality Assurance Scheme for TB sputum microscopy and sputum culture, and had cumulative scores of 100% and 98% for SM and sputum culture, respectively.
The research protocol was approved by the Ethics Committee of the Muhimbili University of Health and Allied Sciences and the Dartmouth Committee for the Protection of Human Subjects.
The sensitivity, specificity, positive and negative predictive values of the sputum smear examinations were calculated by using the sputum culture results as the "gold standard."
Sputum microscopy and sputum culture results on all specimens
Relation of colony forming units (CFUs) on culture to sensitivity of smear microscopy in 212 sputum samples
Quantitative sputum smear results
Quantitative sputum culture results
< 10 afb/100 field
1+ (10–99afb/100 field)
2+ (1–10 afb/field)
3+ (> 10 afb/field)
SM pos %
< 20 CFU
1+ (20–100 CFU)
2+ (> 100 CFU with discrete innumerable colonies)
3+ (> 100 CFU with confluent innumerable colonies)
A total of 103 (4.6%) subjects were sputum culture positive for MTB, 27 (1.2%) were sputum culture positive for NTM and 2 (0.1%) sputum culture positive for both MTB and NTM. Of the 103 subjects sputum culture positive for MTB, 40 (70%) had one positive sputum culture, 30 (29%) had two positive sputum cultures, and 33 (32%) had three positive sputum cultures. The incremental diagnostic yield was 74.8%, 9.7% and 15.5% for the first, second and third sputum culture respectively.
Sputum culture results in patients with negative sputum smears
No. pos sputum cultures
Quantitative sputum culture result
< 20 CFU
> 100 CFU
Clinical, radiological and microbiological findings of 7 patients who had smear positive, culture negative results
1+ (10 – 99 AFB/100 oil fields)
1+ (10 – 99 AFB/100 oil fields)
Cavity on CXR; patient treated for TB and responded
1+ (10 – 99 AFB/100 oil fields)
Patient clinically well. Produced a total of 6 sputum specimens with only one positive smear
1+ (10 – 99 AFB/100 oil fields)
1+ (10 – 99 AFB/100 oil fields)
Abdominal TB samples taken after TB Rx for 2 months.
3AFBs/100 oil fields
Patient clinically well.
6 AFBs/100 oil fileds
2 AFBs/100 oil fields
Fever and peritonitis with ascites by ultrasound, CXR normal, samples taken after 2 months of treatment.
8 AFBs/100 oil fields
Prolonged cough and fever, CXR consistent with TB. Treated for TB and improved remarkably.
(> 10 AFB/oil field)
Culture positive pulmonary TB, repeat samples taken after 2 months of treatment
The data presented here on active TB screening of HIV-infected subjects with CD4 counts > 200 and suspect TB confirm that sputum microscopy is an insensitive tool for TB diagnosis in this setting. In addition the data show that the sensitivity of sputum microscopy is related to the bacterial burden in the positive sputum culture. The high levels of specificity, and positive predictive value of sputum microscopy in this study indicates that a positive test is useful in predicting the presence of pulmonary tuberculosis in HIV-infected individuals. The positive likelihood ratio of the sputum microscopy (235) suggests that a positive test almost rules in the diagnosis. However the negative LR is indicates that the probability of TB is only reduced by half in the face of a negative smear.
The level of sensitivity in the present study (61.8%) is higher than that found in Kenya (33%) , another HIV endemic area, which could be do to the fact that sputum samples in our study were concentrated before examination and possible differences in the degree of immuno-suppression between the studied populations. Eligibility for the study required a CD4 count > 200, a BCG scar, and no evidence of active TB. Thus the population tested may have had different characteristics to those of a general population of HIV positive individuals.
Despite the relatively higher sensitivity seen in our study, almost three quarters of patients with < 20 CFU would be missed by sputum microscopy. The consequences for this are several, including i) delayed or misdiagnosed, contributing to delayed treatment and increased morbidity and mortality rates [13–15]. For example, in the SIMHEALTH 611 study, 60% of the patients in whom the diagnosis of TB was missed, were being treated for non-specific pneumonia prior to death , ii) continued spread of TB to up to 20% of contacts , iii) underestimation of rates of disease and iv) inadequate exclusion of active TB in patients being considered for isoniazid preventive therapy . These facts coupled with the reality that smear-negative TB cases have a poor prognosis  highlight the need for urgent measures to increase detection of smear negative TB cases and to raise the sensitivity of SM. Several laboratory methods have been tried such as sedimentation with either AFB phenol ammonium sulfate  or sodium hypochlorite , use of chitin in mucus digestion  and concentration by centrifugation ,but most of these have limited testing under field conditions.
Most of the patients who had culture positive and smear negative result had discordant culture results. Majority of these patients had only one positive slant that yielded less than 20 colonies per slant, with only a few with three positive slants, which in many cases had a growth of 1+. However, there was no clear pattern of growth for those that yielded two positive slants.
We observed seven cases that had smear positive and culture negative results. Five of these cases were on anti-TB therapy, and it is likely that bacilli seen on the smears were not viable. The other two patients had each only one smear positive result, one had 3 AFB and the other had 1+, and is likely to be due to laboratory contamination. This is due to the fact that they had no signs or symptoms of tuberculosis and subsequently had good clinical outcome without TB therapy. Although the exact causes of these contamination in our laboratory was not identified, it may have resulted from either specimen mix-up, contamination of specimens or reagents with environmental mycobacteria in water, use of single-reagent delivery systems for multiple specimens or contamination in the cabinet area arising from sharing of the facility with several other laboratory workers of different projects. The significant diagnostic yield of the third smear found in this study differs from most other reports and may be a reflection of the particular method of sputum collection used in our protocol. The relatively high diagnostic value of the third smear is surprising because a recent systematic review of 37 studies show the third specimen lead to increase in sensitivity by 3.1% (95% CI, 2.1 to 4.2%) . The second specimen gave surprisingly low incremental yield for both smears and cultures. This scenario may reflect poor instructions for specimens collected at home or very good instructions for the spot specimens and might also be due to mislabeling of the sequence by patients or by improved cough technique by the time of the third specimen. In our study, two samples for sputum microscopy and one for sputum culture would only detect TB in 76 (73.8%) of the 103 cases. However the problem of requesting three specimens would be that some patients may drop out of the diagnostic pathway between submitting specimens and being offered treatment . This problem could be minimized by requesting two smears on the first visit  and by providing patient education coupled with collaboration between treatment supporters, health workers and community members in supervising TB patients .
In summary we have shown that sputum microscopy will only detect slightly more than half of sputum culture positive cases of HIV-associated pulmonary tuberculosis in the setting of active case finding, that sensitivity is related to organism burden and that sputum culture are required for the optimal active case finding of HIV-associated TB.
We would like to acknowledge funding support from National Institutes of Health, DAIDS, AI 45407 and Fogarty International Center, D43-TW006807.
- Perkins MD: New diagnostics tools for tuberculosis. Int J Tuberc Lung Dis. 2000, 4: 182-188.Google Scholar
- Gebre N, Karlsson U, Jonsson G, Macaden R, Wolde A, Assefa A, Miorner H: Improved microscopical diagnosis of pulmonary tuberculosis in developing countries. Trans R Soc Trop Med Hyg. 1995, 89: 191-193. 10.1016/0035-9203(95)90491-3.View ArticlePubMedGoogle Scholar
- Wilkinson D, Sturm AW: Diagnosing tuberculosis in a resource poor setting: the value of sputum concentration. Trans R Soc Trop Med Hyg. 1997, 91: 420-421. 10.1016/S0035-9203(97)90263-7.View ArticlePubMedGoogle Scholar
- Balasangameshwara VH, Chakraborty AK: Validity of case finding tools in a National Tuberculosis Programme. Tubercle. 1993, 74: 52-58.View ArticleGoogle Scholar
- American Thoracic Society: Diagnostic standards and classification of tuberculosis in adults and children. Am J Respir Crit Care Med. 2000, 161: 1376-1395.View ArticleGoogle Scholar
- Murcia-Aranguren MI, Gómez-Marin JE, Alvarado FS, Bustillo JG, de Mendivelson E, Gómez B, León CI, Triana WA, Vargas EA, Rodríguez E: Frequency of tuberculous and non-tuberculous mycobacteria in HIV infected patients from Bogota, Colombia. BMC Infect Dis. 2001, 1: 21-10.1186/1471-2334-1-21. doi: 10.1186/1471-2334-1-21.View ArticlePubMedPubMed CentralGoogle Scholar
- Crampin AC, Floyd S, Mwaungulu F, Black G, Ndhlovu R, Mwaiyeghele E, Glynn JR, Warndorff DK, Fine PEM: Comparison of two versus three smears in identifying culture-positive tuberculosis patients in a rural African setting with high HIV prevalence. Int J Tuberc Lung Dis. 2001, 5: 994-999.PubMedGoogle Scholar
- Rieder HL, Chiang CY, Rusen ID: A method to determine the utility of the third diagnostic and the second follow-up sputum smear examinations to diagnose tuberculosis cases and failures. Int J Tuberc Lung Dis. 2005, 9 (4): 384-91.PubMedGoogle Scholar
- Katamba A, Laticevschi D, Rieder HL: Efficiency of a third serial sputum smear examination in the diagnosis of tuberculosis in Moldova and Uganda. Int J Tuberc Lung Dis. 2007, 11: 659-664.PubMedGoogle Scholar
- Walker D, McNerney R, Mwembo MK, Foster S, Tihon V, Godfrey-Faussett P: An incremental cost-effectiveness analysis of the first, second and third sputum examination in the diagnosis of pulmonary tuberculosis. Int J Tuberc Lung Dis. 2000, 4: 246-251.PubMedGoogle Scholar
- Mtei L, Matee M, Herfort O, Bakari M, Horsburgh CR, Waddell R, Cole BF, Vuola JM, Tvaroha S, Kreiswirth B, Pallangyo K, von Reyn CF: High rates of clinical and sub-clinical tuberculosis among HIV-positive ambulatory subjects in Tanzania. Clin Infect Dis. 2005, 40: 1500-1507. 10.1086/429825.View ArticlePubMedGoogle Scholar
- Githui W, Nunn P, Juma E, Karimi F, Brindle R, Kamunyi R, Gathua S, Gicheha C, Morris J, Omwega M: Cohort study of HIV-positive and HIV-negative tuberculosis, Nairobi, Kenya: comparison of bacteriological results. Tuber Lung Dis. 1992, 73: 203-209. 10.1016/0962-8479(92)90087-Z.View ArticlePubMedGoogle Scholar
- Banda H, Kang'ombe C, Harries AD, Nyangulu DS, Whitty CJ, Wirima JJ, Salaniponi FM, Maher D, Nunn P: Mortality rates and recurrent rates of tuberculosis in patients with smear-negative pulmonary tuberculosis and tuberculous pleural effusion who have completed treatment. Int J Tuberc Lung Dis. 2000, 4: 968-974.PubMedGoogle Scholar
- Elliott AM, Halwiindi B, Hayes RJ, Luo N, Mwinga AG, Tembo G, Machiels L, Steenbergen G, Pobee JO, Nunn PP: human immunodeficiency virus on mortality of patients treated for tuberculosis in a cohort study in Zambia. Trans R Soc Trop Med Hyg. 1995, 89 (1): 78-82. 10.1016/0035-9203(95)90668-1.View ArticlePubMedGoogle Scholar
- Demissie M, Lindtjørn B, Berhane Y: Patient and health service delay in the diagnosis of pulmonary tuberculosis in Ethiopia. BMC Public Health. 2002, 2 (1): 23-10.1186/1471-2458-2-23.View ArticlePubMedPubMed CentralGoogle Scholar
- Murray J, Back P, Lowe P, Coetzee L: Clinico-pathological Study to reduce the rate of missed and misdiagnosis of Pulmonary Tuberculosis in the South African Mining Industry. SIMRAC Project Health. 2000, 611-Google Scholar
- Behr MA, Warren SA, Salamon H, Hopewell PC, Ponce de Leon A, Daley CL, Small PM: Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli. Lancet. 1999, 353: 444-449. 10.1016/S0140-6736(98)03406-0.View ArticlePubMedGoogle Scholar
- Mendelson M: Diagnosing tuberculosis in HIV-infected patients: challenges and future prospects. Brit Med Bull. 2007, 81–82 (1): 149-165. 10.1093/bmb/ldm009. doi:10.1093/bmb/ldm009.View ArticlePubMedGoogle Scholar
- Hargreaves NJ, Kadzakumanja O, Whitty CJ, Salaniponi FM, Harries AD, Squire SB: 'Smear-negative' pulmonary tuberculosis in a DOTS programme: poor outcomes in an area of high HIV seroprevalence. Int J Tuberc Lung Dis. 2001, 5 (9): 847-854.PubMedGoogle Scholar
- Selvakumar N, Rahman F, Garg R: Evaluation of the Phenol Ammonium Sulfate Sedimentation Smear Microscopy Method for Diagnosis of Pulmonary Tuberculosis. J Clin Microbiol. 2002, 40: 3017-3020. 10.1128/JCM.40.8.3017-3020.2002.View ArticlePubMedPubMed CentralGoogle Scholar
- Van Deun A, Maug AK, Cooreman E, Hossain MA, Chambuganj N, Rema V, Marandi H, Kawria A, Portaels F: Bleach sedimentation method for increased sensitivity of sputum smear microscopy: does it work?. Int J Tuberc Lung Dis. 2000, 4: 371-376.PubMedGoogle Scholar
- Farnia P, Mohammadi F, Zarifi Z, Tabatabee DJ, Ganavi J, Ghazisaeedi K, Farnia PK, Gheydi M, Bahadori M, Masjedi MR, Velayati AA: Improving sensitivity of direct microscopy for detection of acid-fast bacilli in sputum: use of chitin in mucus digestion. J Clin Microbiol. 2002, 40: 508-511. 10.1128/JCM.40.2.508-511.2002.View ArticlePubMedPubMed CentralGoogle Scholar
- Bruchfeld J, Aderaye G, Palme IB, Bjorvatn B, Kallenius G, Lindquist L: Sputum concentration improves diagnosis of tuberculosis in a setting with a high prevalence of HIV. Trans R Soc Trop Med Hyg. 2000, 94: 677-680. 10.1016/S0035-9203(00)90230-X.View ArticlePubMedGoogle Scholar
- Mase SR, Ramsay A, Ng V, Henry M, Hopewell PC, Cunningham J, Urbanczik R, Perkins MD, Aziz MA, Pai M: Yield of serial sputum specimen examinations in the diagnosis of pulmonary tuberculosis: a systematic review. Int J Tuberc Lung Dis. 2007, 11 (5): 485-95.PubMedGoogle Scholar
- Squire SB, Belaye AK, Kashoti A, Salaniponi FML, Mundy CJF, Theobald S, Kemp J: 'Lost' smear-positive pulmonary tuberculosis cases: where are they and why did we lose them?. Int J Tuberc Lung Dis. 2005, 9 (1): 25-31.PubMedGoogle Scholar
- WHO Scientific and Technical Advisory Committee on TB (STAG): Improving the diagnosis and treatment of smear-negative pulmonary and extrapulmonary tuberculosis among adults and adolescents. Recommendations for HIV-prevalent and resource-constrained settings. [http://www.who.int/tb/publications/2006/tbhiv_recommendations.pdf]
- Wandwalo E, Makundi E, Hasler T, Odd Morkve O: Acceptability of community and health facility-based directly observed treatment of tuberculosis in Tanzanian urban setting. Health Policy. 2005, 78: 284-294. 10.1016/j.healthpol.2005.11.010.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2458/8/68/prepub
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