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Improving child health promotion practices in multiple sectors – outcomes of the Swedish Salut Programme

Abstract

Background

To improve health in the population, public health interventions must be successfully implemented within organisations, requiring behaviour change in health service providers as well as in the target population group. Such behavioural change is seldom easily achieved. The purpose of this study was to examine the outcomes of a child health promotion programme (The Salut Programme) on professionals’ self-reported health promotion practices, and to investigate perceived facilitators and barriers for programme implementation.

Methods

A before-and-after design was used to measure programme outcomes, and qualitative data on implementation facilitators and barriers were collected on two occasions during the implementation process. The sample included professionals in antenatal care, child health care, dental services and open pre-schools (n=144 pre-implementation) in 13 out of 15 municipalities in a Swedish county. Response rates ranged between 81% and 96% at the four measurement points.

Results

Self-reported health promotion practices and collaboration were improved in all sectors at follow up. Significant changes included: 1) an increase in the extent to which midwives in antenatal care raised issues related to men’s violence against women, 2) an increase in the extent to which several lifestyle topics were raised with parents/clients in child health care and dental services, 3) an increased use of motivational interviewing (MI) and separate ‘fathers visits’ in child health care 4) improvements in the supply of healthy snacks and beverages in open pre-schools and 5) increased collaboration between sectors. Main facilitators for programme implementation included cross-sectoral collaboration and sector-specific work manuals/questionnaires for use as support in everyday practice. Main barriers included high workload, and shortage of time and staff.

Conclusion

This multisectoral programme for health promotion, based on sector-specific intervention packages developed and tested by end users, and introduced via interactive multisectoral seminars, shows potential for improving health promotion practices and collaboration across sectors. Consideration of the key facilitators and barriers for programme implementation as highlighted in this study can inform future improvement efforts.

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Background

To improve health in the population, public health innovations must be successfully implemented within organisations, requiring behaviour change in health service providers as well as in the target population group. However, the literature to date indicates that such change is seldom easily achieved [15]. Numerous theories on behaviour change have been developed over the years [6], but there are no easy to follow recipes on how to achieve uptake of new public health innovations. This is because a number of different factors, often in interaction, will affect how innovations are disseminated, implemented and sustained in practice, and the influence of each factor is highly context-dependent [7]. Furthermore, features of the innovation itself have been shown to play a crucial role in individuals’ decisions to adopt or reject it. Innovations characterised by high relative advantage, compatibility, trialability, observability, and less complexity are more likely to be adopted than other innovations [8]. Innovations in health promotion and disease prevention are often characterised by low relative advantage, as outcomes are often revealed, if at all, after a long period of time. This partly explains why such innovations generally diffuse more slowly than innovations in other fields [9].

Adapting innovations to the local context is described as essential to improve uptake, a process that also involves an assessment of factors that can facilitate or impede change [10, 11]. When planning for complex changes, Grol and Wensing emphasize the importance of considering factors at different levels, including features of the innovation; the professionals and patients; and the social, organisational, economic and political context [12]. Conceptual models, for example as developed by Greenhalgh et al., can also be used as tools to facilitate consideration of the situational complexity and the interaction of different factors [7]. Both quantitative and qualitative measures can contribute in illuminating factors that can facilitate or impede change. However, the use of qualitative methods in organisation and practice change trials has so far been relatively uncommon. This means that it is seldom known why an intervention was successful or not [13], hindering further learning, development and improvement [12, 14]. To date, there are few implementation studies of complex public health innovations that span different organizations and involve other professions than physicians [7, 15].

In 2005, the Västerbotten County Council in Sweden launched the Salut Programme with the aim to develop and strengthen health-promoting activities in health care, social services and school settings, and increase collaboration between these sectors [16, 17]. The programme starts with the pregnant woman and her partner and continues to follow the child up to the age of 18 via age-adapted interventions grouped into modules. Interventions in the programme’s first two modules (from pregnancy to 18 months of age) were developed and tested in four pilot areas from 2005 to 2007 [16]. From 2008 to 2010 they were implemented in the rest of the county in three sequences. This study investigated the early outcomes of this attempt to improve health promotion practices and collaboration between antenatal care (ANC), child health care (CHC), dental services and open pre-schools. A more detailed description of involved sectors and the Salut Programme development can be found elsewhere [5, 16, 17]. The second (coastal areas) and third (inland areas) implementation sequences, involving 13 out of 15 municipalities in Västerbotten, were monitored for the purpose of this study.

The overall aim was to examine outcomes of the Salut Programme on professionals’ self-reported health promotion practices and to investigate perceived facilitators and barriers for programme implementation. The research questions were:

  1. I.

    Are there significant changes in professionals’ self-reported health-promoting practices following programme implementation?

  2. II.

    Are there significant changes in professionals’ self-reported collaboration between sectors following programme implementation?

  3. III.

    What are the main facilitators and barriers for programme implementation as perceived by professionals, and do these differ between the time of initiation and completion, or between sectors?

Methods

Study design

A before-and-after design was used to measure programme outcomes, and qualitative data on implementation facilitators and barriers were collected on two occasions during the implementation process. Involved sectors were ANC, CHC, dental services and open pre-schools in 13 out of 15 municipalities in Västerbotten county (260,000 residents), Sweden.

The Salut Programme implementation

The Salut Programme implementation process included four full-day seminars with lectures and intra- and inter-sectoral group discussions. Professionals were provided with sector specific work manuals for use in everyday practice and questionnaires for use as epidemiological surveillance tools. The questionnaires also served as a base for dialogues on health and lifestyle in ANC and dental services. Small scale testing of interventions between seminars was encouraged, and meetings conducted if necessary. The sector specific intervention packages have been described in more detail elsewhere [16]. The timeline for programme implementation, data collection, and the content of seminars are summarized in Table 1.

Table 1 Timeline for programme implementation, data collection, and content of seminars 1-4 including approximate activity durtion 1

Data collection

Surveys

Two surveys were developed and used in the study. Survey 1 included questions on health promotion practices, knowledge and attitudes in relation to lifestyle counselling, and extent of collaboration between sectors (23–27 questions depending on professional group). The questions were inspired by the Swedish version of The WHO Collaborative Study Questionnaire for GPs [18], but the questions were adjusted to fit the study setting and to reflect the intended goals of the Salut Programme (Table 2). The following domains were explored: 1) Respondents in ANC, CHC and dental services were asked how many of their 10 latest clients they had raised different lifestyle topics with (see Table 3 for sector-specific topics) and 2) to what extent they had used motivational interviewing (MI) [19] in lifestyle counselling. 3) Respondents in CHC were asked to what extent they offered EPDS-screening post partum [20] and 4) to what extent they invited fathers for a separate visit during the child’s first 18 months. 5) Respondents in open pre-schools were asked how often they offered outdoor activities, 6) activities encouraging physical activity in children, and 7) they were also asked to provide information on snacks and beverages provided at pre-schools (tick box question; Table 4). 8) Finally, all respondents were asked about the extent of collaboration with other sectors (each sector named). The questions had five response alternatives ranging from ‘no client’ to ‘all clients’ (Q1), from ‘never’ to ‘always’ (Q2-6), and from ‘not at all’ to ‘to a very large extent’ (Q8).

Table 2 Västerbotten County Councils public health vision, and goals of the Salut Programme
Table 3 Change in professionalspractices of raising different lifestyle topics in encounters 1
Table 4 Supply of snacks and beverages at open pre-schools pre- and post- implementation 1 (n=20)

Survey 2 included 16 open-ended questions on perceived implementation facilitators and barriers. The questions were guided by the framework of Grol and Wensing who propose factors to be identified at several levels of the healthcare system [12]. An initial question asked respondents to describe their workplace’s preconditions for Salut Programme implementation, and thereafter perceived implementation facilitators and barriers in relation to: the programme content and implementation process; the situation, routine and practices of work; the clients; the immediate organisational environment (colleagues, managers/directors, working climate etc.); collaborating actors; the organisational preconditions (structure, resources, administration etc.) and current activities in the society. The final question gave the respondents an opportunity to add other important aspects.

Survey 1 was pre-tested by 12 professionals (three per sector) and Survey 2 by three professionals outside the study areas, which resulted in minor linguistic adjustments.

Procedure

Survey 1 included serial numbers to allow for pre- and post- implementation comparisons and was sent by mail to the respondent’s work address prior to their first encounter with the Salut Programme, and 4–6 months after programme implementation. Three reminders were used, two via mail (the second with a new survey form), and one via telephone. All respondents that returned a completed survey form received a movie ticket, two if they answered both the pre- and post surveys. Survey 2 was administered at seminars 2 and 4 with respondents given 20 minutes to write their responses. It was anonymous, with respondents only being asked to report their profession, allowing for group comparisons only between time points. The timeline for data collection is illustrated in Table 1.

Study sample

The study sample (n=144) consisted at pre-implementation of midwives (n=33), child health nurses (n=66), dental hygienists/dental nurses (n=21) and open pre-school teachers (n=24). Survey 1 was sent to all professionals in ANC and CHC in the 13 municipalities, and to all professionals in dental services clinics and open pre-schools that had agreed to participate in the Salut Programme. The response rate for Survey 1 at pre-implementation was 93%, and 81% of those who replied, also responded post-implementation (Figure 1). Change of employer or retirement were the main reasons for loss to follow up. All professionals who attended learning seminar 2 (n=148) and 4 (n=116) were asked to complete Survey 2. The response rates for Survey 2 were 96% (n=142) at seminar 2 and 85% (n=98) at seminar 4.

Figure 1
figure 1

Recruitment and response rates ( Survey 1 ).

Data analyses

Quantitative data from Survey 1 were analysed with SPSS Statistics software (version 19). Descriptive statistics were calculated, and non-parametric techniques were used to evaluate significant changes from pre- to post- implementation as all outcome measures were based on ordinal and nominal data. The Wilcoxon Signed Rank Test was used to evaluate significant changes in health promoting practices and extent of collaboration between sectors and the McNemar Test was used to explore significant changes in supply of snacks and beverages at open pre-schools. Due to the requirements of both tests, professionals who did not respond both pre- and post- implementation were excluded from the analyses. To minimise the risk of false positive results due to multiple comparisons, statistical significance was defined as p≤.01.

Qualitative content analysis [21] was used to examine the free-text answers to the open-ended questions in Survey 2. Firstly, the answers from all professional groups were read through to get a sense of the whole and also the variation in responses. Secondly, facilitators and barriers were identified, listed, abstracted into categories, and these were then sorted according to the levels suggested by Grol and Wensing, i.e. the innovation, the individual professional, the client, and the social, organisational and the economic and political context [12, 22]. The first author (KE) performed the initial analysis of all answers, and randomly selected sections were analysed by a co-author (EE). Any deviating interpretations were discussed and the categories slightly revised, resulting in a reassessment of free text answers. Finally, the number of respondents contributing to each category was counted and proportions reported. For conciseness, a decision was made to present the six most frequently described facilitators and barriers in each sector as these accounted for the majority of written responses.

Ethics

The study was carried out in compliance with the ethical principles presented in the Helsinki Declaration. Ethics approval was obtained from the Ethics Review Board of Umeå University, Sweden (Ref. 08-168Ö).

Results

Sample characteristics

As shown in Table 5, the mean age ranged from 44 to 52 years (depending on professional group), all were females except two child health nurses, and the mean number of years in profession ranged from 8 to 19 years between different groups. The proportion of respondents working full time ranged from 44% (midwives) to 75% (dental hygienists/dental nurses). Most child health nurses (93%) had other work tasks than child health care, while 22% of midwives had other work tasks than antenatal care (Table 5).

Table 5 Sample characteristics (n=134)

Programme outcomes: changes in health promotion practices and collaboration (survey 1)

Health promotion practices in individual encounters

As shown in Table 3, significant improvements were found in several areas within CHC and dental services, and in one topic area, ‘men’s violence against women and children’ within ANC. Some areas showed a decrease, but this was not statistically significant. The percentages of midwives who reported raising different topics with ‘more than half or all pregnant women’ were already high before programme implementation. Child health nurses reported significantly improved practices for raising issues related to men’s violence against women and children, drug use, discretionary foods (adults), five fruits and vegetables a day (adults and children), 30 minutes daily physical activity and tooth brushing/dental health (children). Dental hygienists and dental nurses reported significant improvements in their practice for raising issues related to weight/BMI, discretionary foods, regular meals, beverage intake, and intake of five fruits and vegetables a day – representing major improvement in the majority of investigated areas in dental services.

Using motivational interviewing (MI) in lifestyle counselling

Child health nurses significantly improved their practice of using MI in lifestyle counselling (p=.006), with an increase from 51% to 60% reporting that they ‘often’ or ‘always’ used MI. Non- significant changes were found in ANC and dental services, with increases from 46% to 55% and from 61% to 67% for the two sectors respectively.

Inviting fathers to a CHC visit and screening mothers for postpartum depression (EPDS)

Child health nurses significantly improved their practices of inviting fathers for a separate visit during the child’s first 18 months (p=.000), with an increase from 0% to 38% reporting that they ‘often’ or ‘always’ invited fathers for a separate ‘father’s’ visit. There was a non-significant increase in EPDS-screening (p=.030), with 98% reporting that they ‘often’ or ‘always’ performed EPDS-screening post-implementation compared with 85% pre-implementation. However, unlike the EPDS-screening, inviting fathers for separate visits was a new work routine introduced as part of the programme.

Open pre-schools’ supply of healthy food and activities to encourage physical activity

There was a significant reduction in the reported supply of cookies, biscuits and sweet buns and sweetened beverages in open pre-schools (Table 4). However, no significant changes in activities to encourage children’s physical activity (p=.414) or outdoor activities (p=.107) were found. Only 15% of open pre-school teachers reported that outdoor activities were offered ‘often’ or ‘always’ at follow-up, an increase from 5%, while offering activities encouraging physical activity ‘often’ or ‘always’ decreased from 75% to 70%.

Collaboration between sectors

Table 6 shows that midwives significantly increased their collaboration with dental services, less so and not significantly with open pre-schools. Most midwives reported a high degree of collaboration with CHC both pre- and post- implementation. Child health nurses also significantly increased their collaboration with dental services. Most child health nurses reported a high degree of collaboration with ANC, consistent with the information reported by midwives. Dental hygienists and dental nurses significantly increased their collaboration with ANC and CHC, less so and not significantly with open pre-schools. Open pre-school teachers significantly increased their collaboration with dental services, with an increase from 10% to 60% reporting the collaboration to be considerable or very considerable.

Table 6 Change in professionalscollaboration between sectors 1

Perceived facilitators and barriers for programme implementation (Survey 2)

Perceptions about facilitators and barriers were largely shared across sectors, although some sector-specific factors were found. The six most frequently reported facilitators and barriers are presented for each professional group in Tables 7 and 8. The tables also provide information on changes in the frequency with which each factor was reported between the two occasions (Seminar 2 and 4).

Table 7 The six most frequently reported facilitators for implementation in ANC, CHC, dental services and open pre-schools
Table 8 The six most frequently reported barriers for implementation in ANC, CHC, dental services and open pre-schools

Facilitators

The pattern of reported facilitators differed from that of barriers as they were predominantly identified at the level of the intervention and the social context. The aspects of ‘good collaboration with other sectors’ were frequently reported, a factor that seemed to gain in importance by the end of the implementation process (Seminar 4) in ANC, CHC and open pre-schools. The importance of having positive and supportive colleagues and working climate was also reported as an important facilitator across sectors. All sectors reported the programme to be in line with existing work routines, and ANC, CHC, and dental services reported good support from manuals and questionnaires. Professionals in CHC, dental services and open pre-schools reported that parentsown willingness to change and health consciousness was important. Having positive and supportive managers was among the main facilitators in ANC and dental services, although more frequently reported at Seminar 2. ANC also emphasised the importance of professionals working in the same way towards a shared goal. CHC perceived the programme to be clear and relevant. Open pre-school teachers stated that being positive towards the programme/intervention facilitated implementation. Geographical proximity was also reported among the main facilitators in open pre-schools. Interestingly, in all sectors organisational level and economic and political level facilitators were scarcely mentioned.

Barriers

Workload and shortage of both time and staff was the most frequently reported barrier, although the frequency with which this factor was reported decreased over time in all sectors except for open pre-schools. Difficulties to start or maintain collaborative relations were also a common barrier that seemed to increase in importance by the end of the implementation process (Seminar 4). Other main barriers reported were: collaborative partners were missing (ANC, CHC); geographical distance to collaborators (ANC, CHC, and open pre-schools); insufficient managerial involvement and support (CHC, dental services and open pre-schools). Competing goals, demands and work tasks posed a main barrier in ANC, CHC and dental services, although decreasing over time (in Survey I, 93% of the child health nurses reported having other work tasks than CHC (Table 5)). Lack of motivation, interest, time or capacity/resources among parents/clients were barriers experienced in dental services and open pre-schools, especially during the initiation phase (Seminar 2).

Some sector-specific barriers were found: The perception that new topics and questionnaires could be perceived as sensitive/intrusive or too extensive by expectant parents did seem to raise concern in ANC, especially by the end of the implementation process (Seminar 4) when this barrier was reported by half of the midwives. Lack of or conflicting financial incentives was reported in dental services, but mainly seemed to raise concern during the initiation phase (Seminar 2). Insufficient work facilities and supplies were mentioned as a barrier by almost half of the responding open pre-school teachers at Seminar 2. This was partly due to the fact that outdoor activities were suggested as one of the programme interventions, but many open pre-schools did not have sufficient or safe out-door environments to carry out these activities.

Discussion

This study aimed to examine outcomes of a Swedish child health promotion programme on professionals’ self-reported health promotion practices, and to investigate perceived facilitators and barriers for programme implementation. The results show that self-reported health promotion practices and collaboration were improved to some extent in all sectors at follow up. Main facilitators for programme implementation included cross-sectoral collaboration and sector-specific work manuals/questionnaires for use as support in everyday practice. Main barriers included high workload, and shortage of time and staff. A strength of this study is that it spanned multiple sectors and included both outcome measures and cross sectional data on implementation facilitators and barriers, allowing for a deeper understanding of the context in which changes occurred. It is acknowledged that the outcome data are self-reported and thus susceptible to bias, so due caution is needed in interpreting the results.

The most significant changes were reported in CHC and dental services, and the least in ANC, the sector that may have the greatest opportunity to influence the target group. The results indicated that the majority of Salut activities were already in place within ANC. Midwives normally encounter a pregnant woman seven to ten times during pregnancy. The partners also seem more inclined to be involved in healthcare during pregnancy than after the child’s birth [5, 23]. Thus, ANC might have needed more advanced interventions, especially since midwives have an excellent opportunity to involve both parents in health promotion activities. Interestingly, this was an issue raised by the midwives, as insufficient routines for involvement of fathers were reported as one of the barriers for programme implementation in ANC. A possible explanation for the relatively large change in dental services might be a novelty effect due to the introduction of new topics, or a simpler, less comprehensive intervention. The latter might explain why at the end of the implementation process (Seminar 4) fewer professionals in dental services reported support from structured work manuals as an important facilitator.

The framework used for analysing facilitators and barriers for change was valuable as it recognizes the importance of giving attention to different organisational levels [12]. During improvement efforts the focus is often on factors related to the individual professional [6] but as shown in this study, a number of factors can be involved and interact at multiple levels, many out of reach for the individual professional to influence.

Cross-sectoral collaboration emerged as one of the main facilitators for programme implementation. It is likely that the approach of gathering all sectors for seminars was successful in providing professionals with the opportunity to meet, interact and initiate or increase their collaboration, which in turn can create synergies and allow for a more integrated approach in provision of services [24, 25]. Among the main facilitators identified were also the sector specific and structured work manuals and questionnaires. These results are consistent with findings from a previous study where the development of patient education toolkits and brochures was found to be one crucial component in improving nursing care in hospital and community settings [4].

The Salut Programme interventions were originally developed and tested within each profession in pilot areas before countywide implementation [16]. Thus, contextual factors that could impede change were to some extent already adjusted and accounted for [10, 11, 26, 27]. A recent study of the implementation of a tool for lifestyle interventions in Swedish primary health care shows that a fixed implementation strategy (i.e. no tailoring of activities to meet professional needs) was not successful in terms of uptake of the intervention. Beliefs, values and behaviours linked to professional subcultures influenced the implementation, and most professional groups found the intervention more suitable for other professionals, contributing to a low level of adherence [28]. Thus, adapting approaches to different subgroups, preferably by involving end-users, is a strategy to enhance the likelihood of success [6, 8, 10, 22], which is consistent with our findings.

Another commonly mentioned facilitator across sectors was that interventions were perceived to be in line with existing work routines. This is also an indication that the interventions were well adapted to the target context. However, an alternative interpretation is that the interventions did not introduce any novel practices or activities, possibly just a more structured way of working due to detailed work manuals and questionnaires. This might be one reason for the few significant changes in antenatal care. Since health promotion has a long tradition of being central in the Swedish antenatal care programme [29], midwives were already addressing most issues included in the programme pre-implementation, leaving little room for improvement.

The main barriers for programme implementation, high workload and shortage of time, staff or resources, are among the most well recognized in the implementation literature, and our results are thus consistent with previous research [30, 31]. However, our findings suggest that improvements in health promotion practices and collaboration are possible to achieve, despite the presence of these common barriers. Even though positive outcomes were shown in this study, the characteristics of the barriers described indicate that there might have been a lack of overall organisational readiness for change, an important predictor of effective implementation, especially if changes are complex and the change process involves a great degree of interdependence [32]. It is plausible that outcomes in terms of health promotion practices and collaboration could have been further enhanced if attention had been given to readiness for change at the individual as well as the group and organisational levels. In addition, geographical distance was reported among the most important barriers in ANC, CHC and open pre-schools, a factor that should not be overlooked. Having shared premises has been shown to facilitate information transaction, communication and personal familiarity, while being located in separate buildings can constraint the team’s functioning and effectiveness [16, 31, 33]. Our results are consistent with these previous findings. As also indicated in the study, starting and maintaining new collaborative relations can be challenging, and this organisational network structure can be fragile due to a lack of shared management and due to demands both from both the teams and the organisations in which professionals are working [24]. Perceived barriers such as competing goals, demands and work tasks in ANC, CHC and dental services, or the conflicting financial incentives for performance in dental services were examples of such competing demands.

Methodological considerations

The overall results of Survey I (outcome measure) have to be interpreted with caution, as findings from previous studies show that self-reported measures of adherence to guidelines often exceeds objective rates [34]. Nevertheless, the study validity is strengthened by high response rates (81% to 96%), this being well above what is commonly found in surveys with health care professionals [35]. Several factors known to enhance response rates were adopted in the study [36]. Furthermore, the first author and three of the co-authors participated in seminars and were introduced to all professionals. It is plausible that this familiarization further contributed to the overall high response rates. The risks of making Type I and Type II errors were considered in relation to the multiple comparisons made in the study, and a decreased p-value (p≤.01) for statistical significance was judged to be the most reasonable way to balance these risks.

Confounding factors cannot be readily ‘controlled’ for in implementation studies, as the context itself plays an important role in the level of success [7]. Thus, we do not know the extent to which sector specific continuous development and education contributed to programme outcomes. The results from the answers to the open-ended questions in Survey 2 should also be interpreted with caution, as the extent of responses varied between respondents. However, the research team aimed to enhance the trustworthiness of the analysis by parallel coding and collaboration on categorization.

Conclusions

This multisectoral programme for health promotion, based on sector-specific intervention packages developed and tested by end users, and introduced via interactive multisectoral seminars, shows potential for improving health promotion practices and collaboration across sectors. Consideration of the key facilitators and barriers for programme implementation as highlighted in this study can inform future improvement efforts.

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Acknowledgements

We are grateful to participating professionals and to Magdalena Sundqvist, and the Salut process management team for sharing their time and experiences, and to Anna Cederberg Foundation, JC Kempe Foundation, Medical Faculty at Umeå University, Queen Silvia Jubilee Foundation, Swedish Council for Working Life and Social Research (FAS), Swedish Society of Nursing, Vinnvård, and Wallenberg Foundation for financial support. The study was undertaken within the FAS Centre for Global Health Research at the Medical Faculty of Umeå University in cooperation with the Vinnvård Research Program in Sweden [Grant no A2007034], and Mother and Child Health Research at La Trobe University, Melbourne, Australia.

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Correspondence to Kristina Edvardsson.

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The authors declare that they have no competing interests.

Authors’ contributions

KE, MN, RG, EE, IM and AI designed the study. KE collected the data, KE, EE, MN and ML conducted the analyses and KE drafted the manuscript in close collaboration with MN and RG. RS read and revised the manuscript with specific attention to language and clarity of presentation. All authors contributed in the writing process before the final version was approved. All authors read and approved the final manuscript.

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Edvardsson, K., Ivarsson, A., Garvare, R. et al. Improving child health promotion practices in multiple sectors – outcomes of the Swedish Salut Programme. BMC Public Health 12, 920 (2012). https://doi.org/10.1186/1471-2458-12-920

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