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Mortality rate among HIV-positive children on ART in Northwest Ethiopia: a historical cohort study

Abstract

Background

Though highly active antiretroviral therapy (HAART) has been available for more than a decade in Ethiopia, information regarding mortality rates of human immunodeficiency virus (HIV)-positive children after antiretroviral therapy antiretroviral therapy (ART) initiation is very scarce. Thus, this study intends to determine the predictors of mortality among HIV-positive children receiving ART in Amhara Region.

Methods

A multicenter facility-based historical cohort study was conducted in 538 HIV-positive children on ART from January 2012 to February 2017. We employed a standardized data extraction tool, adapted from ART entry and follow-up forms. Descriptive analyses were summarized using the Kaplan-Meier survival curve and log rank test. Then, the Cox-proportional hazard regression model was employed to estimate the hazard of death up to five-years after ART initiation. Variables with p-values ≤0.25 in bivariable analysis were candidates to the multivariable analysis. Finally, variables with p-values < 0.05 were considered as significant variables.

Results

The cohort contributed a total follow-up time of 14,600 child-months, with an overall mortality rate of 3.2 (95% CI: 2.3, 4.3) per 100 child-years. This study also indicated that HIV-infected children presenting with opportunistic infections (OIs) (AHR: 2.5, 95% CI: 1.04, 5.9), anemia (AHR: 3.1, 95% CI: 1.4, 6.7), severe immunodeficiency (AHR: 4.4, 95% CI: 1.7, 11.7), severe stunting (AHR: 3.3, 95% CI: 1.4, 8.0), severe wasting (AHR: 3.1, 95% CI: 1.3, 7.3), and advanced disease staging (III and IV) (AHR: 3.0, 95% CI: 1.2, 7.1) were at higher risk of mortality.

Conclusion

A higher rate of mortality was observed in our study as compared to previous Ethiopian studies. HIV-positive children presenting with anemia, OIs, severe immunodeficiency, advanced disease staging (III and IV), severe stunting, and severe wasting were at higher risk of mortality.

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Background

Human immunodeficiency virus (HIV) remains a serious public health concern worldwide, but low and middle-income countries (LMICs) are the most affected. In 2018, approximately 37.9 million people were living with HIV worldwide, of whom 1.8 million were children (age < 15 years) [1]. Sub-Saharan Africa (SSA) is a disproportionally affected region contributing to approximately 91% of HIV-positive children in 2012 [2]. Globally, Acquired Immune Deficiency Syndrome (AIDS) remains the leading cause of mortality among children, resulting in 190,000 children deaths in 2013 [3]. In Ethiopia, 109,133 children are living with HIV and approximately 2420 are newly infected with HIV annually [4].

The use of anti-retroviral therapy (ART) has been effective in reducing mortality markedly among infected children and adolescents [5]. In this regard, the World Health Organization (WHO) exceeded its target of 15 million ART users by the end of 2015. Likewise, the Ethiopian government launched cost-based ART in 2003 and cost-free ART in 2005, delivered as part of comprehensive HIV/AIDS care [6]. As a result, ART coverage among children in Ethiopia has increased from 9% in 2013 to 58% in 2018 [7]. According to the recent Ethiopian Demographic and Health Survey (EDHS, 2016), child mortality in Ethiopia was reported as 67 per 1, 000 live births. In our study area (Amhara Region), child mortality rate was reported as 85 per 1, 000 live births [8].

Despite different interventions undertaken by the Ethiopian government, the mortality rate among HIV infected children remains higher than the general pediatric population. Previous studies done in Ethiopia reported that the mortality rate in HIV-positive children receiving ART ranged from 1.4 deaths per 100 child-years of observation [9] to 4 deaths per 100 child-years of observation [5]. These studies also reported that many factors significantly increased the risk of mortality in this population. For example, baseline anemia (Hgb < 10 g/dL) [5, 9], low CD4 count [5], advanced WHO clinical disease staging (III & IV) [5, 9], poor ART drug adherence [10], and malnutrition [11] were reported as factors significantly increasing risk of mortality in HIV-positive children receiving ART.

Obtusely, well-organized and up-to-date information concerning mortality rate of HIV-positive children after ART initiation is highly recommended to provide quality care to HIV-positive children. However, although Amhara region has a particularly high child mortality rate, a minimal number of studies have been conducted in this area. Further, even though a few studies have been conducted in Ethiopia, these studies have failed to consider important predictors, such as malnutrition and developmental status of the children, which significantly affect the mortality rate of HIV-positive children [11, 12]. Thus, this study was conducted to address this knowledge and evidentiary gap by identifying the predictors of mortality among HIV-positive children receiving ART in Amhara Region referral hospitals, Northwest Ethiopia.

Methods

Study settings and period

This study was done in three purposely selected Amhara Region Referral Hospitals between January 2012 and February 2017. The three selected hospitals were Debre Markos Referral Hospital, Felege Hiwot Comprehensive Specialized Hospital, and University of Gondar Comprehensive Specialized Hospital, with respective locations of 299 km, 578 km, and 725 km from Addis Ababa, the capital city of Ethiopia. These hospitals were selected because they provide ART follow-up and care services for a large proportion of HIV-positive patients in the region. Together three hospitals provide inpatient and outpatient services for more than 15 million people living in the Amhara Region and neighboring regions. In addition to other medical services, all the three hospitals provide chronic HIV care (ART) for approximately 1071 children in their catchment areas.

Study design and participants

A multicenter facility-based historical cohort study was undertaken. All children living with HIV receiving ART in all Amhara Region Referral Hospitals were our target population. All randomly selected HIV-positive children who took ART medication for a minimum of 1 month during the follow-up period in the study hospitals were included. Excluded participants were those with incomplete baseline variables.

Sample size and sampling technique

The minimum required sample size was estimated based on the following variables: severe wasting, severe underweight, CD4 counts or percentage below the threshold, anemia (Hgb < 10 g/dL), and WHO clinical stage III and IV. However, the maximum calculated sample size was obtained from CD4 cell count or percentage below the threshold and was taken as our final sample size. Our final sample size was calculated based on the following parameters: P1: percent of exposed (CD4 count or % below the threshold) with outcome (11.6%), P2: percent among non-exposed (CD4 count or % above the threshold) with outcome (4.4%), 95% CI, Zβ = 80% and r = 1: 1 ratio. The final sample size after adding a10% chart incompleteness rate was 553. The parameters were taken from a previous study conducted at Bahir Dar Referral Hospital [5].

First, the medical charts of HIV-positive children started on ART (740) prior to the follow-up period at the three selected hospitals were sorted. Next, 553 children’s medical records were selected using a simple random sampling technique. Lastly, data across 5 years were extracted from each enrolled child’s record (Fig. 1).

Fig. 1
figure 1

Schematic presentation of sampling procedure to assess the predictors among HIV-infected children on ART in Amhara Region Referral Hospitals from January 1, 2012 to February 28, 2017. Footnote: proportion was calculated as follows: the total number of HIV-infected children on ART at a given hospital between January 1, 2012 and February 28, 2017 multiplied by our calculated sample size (553), then divided by the total number of HIV-infected children started ART in the three hospitals between January 1, 2012 and February 28, 2017 (740). For example, for DMRH, it was calculated as: 205*553/740 = 153

Data extraction procedures

After reviewing some HIV-infected children charts and similar articles, a standardized data extraction checklist was carefully developed. The resulting data extraction tool had the following components: sociodemographic characteristics of caregivers and their children and clinical characteristics and nutritional status of the children. Trained data collectors carefully extracted all available data from the selected medical records of HIV-positive children. The most recent laboratory and clinical measurements documented at ART initiation were used as baseline values. A daily base supervision was done by assigned supervisors to maintain data quality. Besides, training concerning data extraction process was given for both data collectors and supervisors. Lastly, the consistency between records and collected data was confirmed by principal investigators through randomly selected reviews of previously extracted medical records.

Measurements

The death of any HIV-positive child after ART initiation during the five follow-up period was considered as an event. Conversely, study participants who were still alive at the end of follow-up, lost from the study, or transferred to other health institutions were considered as censored.

ART drug adherence level was calculated based on the total monthly doses of ART. Good ART adherence was documented when the compliance level was ≥95% or missed doses were ≤ three per month. Fair ART adherence was documented when the compliance level was between 85 and 94% or missed doses were between four and eight per month. Lastly, poor ART adherence was documented when the compliance level was < 85% or missed doses were ≥ nine per month [13].

HIV-positive children with weight for age (W/Age) Z-score < − 2 SD, height for age (H/Age) Z-score < − 2 SD, and weight for height (W/H) Z-score < − 2 SD were classified as moderately underweight, stunted, and wasted respectively [14, 15].

HIV-positive children with W/Age Z-score < − 3 SD, H/Age Z-score < − 3 SD, and W/H Z-score < − 3 SD were classified as severely underweight, stunted, and wasted respectively [14, 15].

The CD4 count below the threshold for ages < 12 months, 12–35 months, 36–59 months, and ≥ 5 years were < 1500/mm3, < 750/mm3, < 350/mm3, and < 200/mm3 respectively [16].

Data management and statistical analysis

Epi-Data™ Version 3.1 was used for data entry and STATA™ Version 14 was used for statistical analysis. Descriptive statistics for continuous variables were summarized using mean, median, and standard deviation. The survival probability among HIV-infected children from the date of ART initiation to event was estimated using the Kaplan Meier survival curve. Cox proportional hazard regression model was fitted, then all variables with p-value ≤0.25 in the bivariable analysis were included in a multivariable model. Cox-proportional hazard model assumption was checked using Schoenfeld residual test for continuous variables and Log-Log plots for categorical variables. Besides, multicollinearity was checked using the variance inflation factor (VIF). In the final model, hazard ratios with 95% confidence intervals and p-values (< 0.05) were used to identify statistically significant predictors and measure the strength of association.

Results

Demographic characteristics of included participants

After reviewing 553 HIV-infected children’s records, 538 records were included in the final analysis as, 15 records were excluded due to incompleteness. About half (51.5%) of study participants were females and the majority of participants (83.6%) were from urban areas. The baseline mean age of the participant cohort was 6.7 years (SD: 3.8 years); with the mean age of caregivers reported as 33.1 years (SD: 0.4 year). Details of another captured socio-demographic characteristics are found in Table 1.

Table 1 Baseline demographic characteristics of HIV infected children receiving ART in Amhara Region Referral Hospitals between January 2012 and February 2017

Baseline clinical, laboratory, and ART information

About 45.0% of the HIV-positive children had opportunistic infections (OIs) at ART initiation. Of these, 23.3, 20.9, and 17.1% of the children had diarrhea, pneumonia, and tuberculosis, respectively (Fig. 2). About 57.6% of the HIV-positive children had mild WHO clinical disease staging (stage I and II). Moreover, nearly two-thirds (69.5%) of the children had CD4 counts below the threshold. Furthermore, less than one-third (30.8%) of the participants had anemia at ART initiation. Regarding baseline nutritional status, 17.3, 22.7, and 16.7% of HIV-infected children were severely underweight, stunted, and wasted. Furthermore, about 12.3% of the children were anemic during ART initiation (Table 2).

Fig. 2
figure 2

Baseline opportunistic infections of HIV-infected children receiving ART in Amhara Region Referral Hospitals from January 1, 2012 to February 28, 2017

Table 2 Clinical, laboratory, and ART information of HIV infected children receiving ART in Amhara Region Referral Hospitals from January 1, 2012 to February 28, 2017

Follow-up characteristics of the children

Throughout the follow-up time, 17.8% of the study participants developed ART related side effects. Additionally, nearly one-fourth (26.4%) of the children developed OIs during the follow-up time. Furthermore, almost one-third (29.7%) of HIV-infected children had a history of ART regimen change during the follow-up time. Interestingly, about 4.7% of the children discontinued their medication during the follow-up time. More than half (59.1%) of the HIV-positive children were taking a combination of zidovudine, lamivudine, and efavirenz or zidovudine, lamivudine, and nevirapine (Fig. 3).

Fig. 3
figure 3

Type of regimens during follow-up among HIV-infected children receiving ART in Amhara Region Referral Hospitals from January 1, 2012 to February 28, 2017

Mortality rate during our follow-up

This cohort contributed a total follow-up time of 14,600 child-months. Among a cohort of 538 HIV-infected children, 38 children (7.1%) died, 33 (6.1%) were lost to follow-up, 81(15.1%) were transferred to other health institutions, and 386 (71.8%) remained in the follow-up. Finally, the combined mortality rate of this cohort was 3.2 (95% CI: 2.3, 4.3) per 100 child-years of observation. The number of deaths within the first three, six, and 12 months of ART follow-up were 19 (50%), 24 (63%), and 26 (68%), respectively. The 60 months cumulative survival probability of HIV-infected children was 0.88 (95% CI: 0.84, 0.92) (Fig. 4).

Fig. 4
figure 4

The overall Kaplan-Meier survival curve with 95% confidence intervals of HIV-infected children receiving ART in Amhara Region Referral Hospitals from January 1, 2012 to February 28, 2017

Predictors of mortality

In the final model, the following factors increased the risk of mortality among HIV-positive children: baseline OIs, anemia, severe immunodeficiency, advanced disease stage, severe stunting, and severe wasting. We observed that the hazard of death among children presenting with OIs at ART initiation was 2.5 fold (AHR: 2.5, 95%CI: 1.04, 5.9) higher than to those presenting without OIs. Additionally, the hazard of death among children who initiated ART with severe disease was three-fold higher as compared to those who initiated ART with mild disease (AHR: 3.0, 95% CI: 1.2, 7.1). Furthermore, the hazard of death among children who had severe immunodeficiency was 4.4 fold (AHR: 4.4, 95% CI: 1.7, 11.7) higher than to those who had mild immunodeficiency. The hazard of death among anemic children was three-fold (AHR: 3.1, 95% CI: 1.4, 6.7) higher as compared to non-anemic children. Lastly, the hazard of death among severely stunted, and severely wasted children was three times higher as compared to their well-nourished counterparts [(AHR: 3.3, 95% CI: 1.4, 8.0), and (AHR: 3.1, 95% CI: 1.3, 7.3)] respectively (Table 3).

Table 3 The bivariable and multivariable Cox regression analysis of predictors of mortality among HIV infected children receiving ART in Amhara Region Referral Hospitals from January 1, 2012 to February 28, 2017

Discussion

This study identified the predictors of mortality among HIV-positive children receiving ART using a multicenter facility-based historical cohort study. Within a total follow-up time of 14,600 child-months, the mortality rate of HIV-positive children receiving ART in our study hospitals was 3.2 (95% CI: 2.3, 4.3) per 100 child-years of observation. Our finding is comparable with studies reported from Arba Minch Town Public Health Facilities, Gamo Gofa Zone, Southern Ethiopia (3.1 deaths per 100 child-years) [17], Nigeria (3.0 deaths per 100 child-years) [18], and Andhra Pradesh, India (3.0 deaths per 100 child-years) [19].

Nonetheless, our finding is lower than studies conducted in Addis Ababa Public Hospitals, Ethiopia (12.4 deaths per 100 child-years) [20], Debre Tabor General Hospital and Dessie Referral Hospital, Ethiopia (6.3 deaths per 100 child-years) [21], and Kenya (8.4 deaths per 100 child-years) [22]. These significant disparities across the reported studies could be due to the differences in sample size, study setting, follow-up period, and clinical characteristics of the study participants. As an example, the follow-up period for a study done at Debre Tabor General Hospital and Dessie Referral Hospital was 120 months [21], whereas the follow-up period of our study was 60 months. It is well understood that as the follow-up period increases, the number of events (death) also increases. Additionally, a study conducted in Kenya included only 135 HIV-infected children [22]; however, our study included 538 HIV-infected children with the smaller sample size potentially yielding a less precise estimation.

This study also found a high rate of mortality when compared to studies reported from Adama Referral Hospital and Medical College, Ethiopia (2.1 deaths per 100 child-years) [23], Zambia (1.6 deaths per 100 child-years) [24], and Malawi, Lesotho, and Swaziland (2.25 deaths per 100 child-years) [25]. The above variations might be due to the differences in clinical characteristics of the study participants and study settings as our study included only referral hospitals. Additionally, our study’s higher rate of mortality could be attributed to the clinical characteristics of the included study participants such as the nearly two-thirds (69.5%) of our study participants having severe immunodeficiency.

Of 38 total deaths, 68% occurred within the first year of ART initiation. This finding is comparable with a study done at Bahir Dar Referral Hospital, Ethiopia, found that the mortality rate within the first year after ART commencement was 90.2% [5]. Moreover, this finding is consistent with studies done in other SSA countries, which documented that the death of most HIV-positive children commonly happened in the early phase of ART [26, 27]. Higher premature mortality in our study might be associated with the clinical characteristics of included participants as over two-thirds (69.5%) of the study participants had CD4 counts below the threshold, a population which usually experiences higher early mortality after ART initiation. Moreover, children with weakened immunity are more easily attacked by different types of OIs, which are the most common causes of premature death.

This study found that the risk of mortality among anemic HIV-positive children was three-fold higher as compared to their non-anemic counterparts. Previous Ethiopian studies also documented that anemia had a significant impact on the survival of HIV-infected children [5, 9, 10, 12]. Additionally, studies reported from other LMICs also reported that anemia at ART initiation was significantly associated with higher mortality [22, 27, 28]. Furthermore, studies conducted elsewhere also demonstrated that anemia is the most common hematologic abnormality among people living with HIV. It is significantly associated with low quality of life and a higher risk of mortality [29, 30]. The most common cause of anemia among people living with HIV is the side effects of zidovudine, which 59.1% of our study participants were taking a combination of Zidovudine, lamivudine, and efavirenz or Zidovudine, lamivudine, and nevirapine. Cotrimoxazole preventive therapy (CPT), being reflected in 63.2% of our participants, can be an additional cause of anemia among HIV-positive children.

This study also revealed that HIV-positive children presenting with OIs at ART initiation were at higher risk of mortality. A Tanzanian study also reported that children who had TB and other OIs at ART initiation were at higher risk of mortality [28]. Besides, a study from South Africa also demonstrated that OIs in the form of chronic diarrhea was a significant predictor of mortality [26]. As OIs are the predominant causes of morbidity and mortality among HIV-infected patients, the Ethiopian National ART Guidelines strongly recommends treating OIs before ART initiation [6]. In addition to the direct effect of these OIs, patients may have died due to the side effects of common drugs used to treat these OIs. Concerning drug-drug interactions, studies suggested that HIV-infected individuals treated with anti-TB medications usually experience drug toxicity as compared to HIV-uninfected individuals [31, 32]. As an example, a study done in Canada revealed that HIV-infected people treated with anti-TB medications were more likely to develop drug-related adverse events [32].

Further finding of this current study demonstrated that children who had severe immunodeficiency were at higher risk of death as compared to those who had mild immunodeficiency. This finding is in agreement with the previous Ethiopian studies [5, 9, 12, 21]. Besides, a Brazilian study also reported that baseline CD4 count below 15% significantly increased the hazard of death among HIV-positive children [33]. Similarly, studies conducted elsewhere have documented that HIV-positive children with low CD4 count were at higher risk of mortality than those who had high CD4 count [28, 34,35,36]. Generally, serious and life-threatening OIs, including CNS toxoplasmosis and cryptococcal meningitis are frequent among people having low CD4 count or percentages which increases their vulnerability to death.

Another finding of this study revealed that the death rate among children with advanced disease stage (WHO stage III and IV) was higher as compared to those children with mild disease stage (WHO stage I and II). This finding is highly supported by previous studies conducted elsewhere [9, 10, 22, 23, 37, 38]. OIs are the most common morbidities among HIV-positive children with advanced WHO clinical disease stage, which indicates that the risk of developing and recurrence of OIs increases as the WHO clinical staging becomes more advanced. Hence, people living with HIV commonly died due to OIs rather than HIV/AIDS.

Finally, this study found that undernutrition in the form severe stunting (H/Age Z-score < − 3 SD) and severe wasting (W/H Z-score < − 3 SD) significantly increased the risk of mortality among HIV-infected children on ART. This finding is comparable with the previous Ethiopian studies conducted elsewhere [11, 12]. Additionally, a Kenyan study also revealed that those children who had baseline WHZ (Weight for Age Z-score) < − 2 were at higher risk of death among HIV-positive children receiving HAART [22]. Moreover, a Tanzanian study also found that WHZ and BAZ (Body Mass Index for Age Z-score) of ≤ -2 significantly associated with higher risk mortality within the first 3 months of ART initiation [28]. Generally, the causes of undernutrition among HIV-patients are multidimensional and multifactorial. HIV decreases food consumption as a result of swallowing difficulty caused by oral trash or esophageal candidiasis, reduces nutritional absorption caused by gastrointestinal mucosal damage, and increases the metabolic demands [39, 40].

Limitations of the study

The major limitation of this study was the lack of quality data to verify death status, especially for those who died at home. This gap does not only affect the loss to follow-up, but also may be an under-reporting of outcomes of community-based individuals. In addition, this study did not incorporate important predictors, like viral load and micronutrient deficiency due to incomplete recording system. Finally, early mortality could be underestimated since the study excluded children’s who had ART follow-up was less than 1 month.

Conclusions

A higher rate of mortality was observed in our study as compared to previous Ethiopian studies. Anemia, OIs, low CD4 counts or percentages, advanced disease staging (III and IV), severe stunting, and severe wasting were factors found as significantly increasing the risk of mortality among HIV-infected children receiving ART. Therefore, clinicians should emphasize early screening, managing OIs, and maximizing nutritional supplements for HIV infected children to improve the survival rate of those individuals. Additionally, clinicians should give more emphasis to malnourishment in HIV-infected children at ART initiation. Furthermore, greater attention and close follow-up shall be given for HIV-infected children in the early ART phase.

Availability of data and materials

Data will be available upon request of the corresponding author.

Abbreviations

AIDS:

Acquired Immune Deficiency Syndrome

AZT- 3TC-EFV:

Zidovudine plus Lamivudine plus Efavirenz

AZT-3TC-NVP:

Zidovudine plus Lamivudine plus Nevirapine

BAZ:

Body Mass Index for Age Z-score,

HAART:

Highly Active Antiretroviral Therapy

HAZ:

Height for Age Z-score

Hgb:

Hemoglobin

HIV:

Human Immunodeficiency Virus

SSA:

Sub-Saharan Africa

WAZ:

Weight for Age Z-score

WHO:

World Health Organization

WHZ:

Weight for Height Z score

References

  1. Joint United Nations Programme on HIV and AIDS: Global HIV & AIDS statistics −2019 fact sheet. 2019. available at https://www.unaids.org/en/resources/fact-sheet. Accessed 11 Feb 2020.

    Google Scholar 

  2. Joint United Nations Programme on HIV/AIDS. Fact sheet; 2014. p. 1–6. available from http://www.unaids.org/en/media/unaids/contentassets/documents/factsheet/2014/20140716_FactSheet_en.pdf.

    Google Scholar 

  3. UNICEF: Unicef Annual Report 2015. available from https://www.unicef.org/publications/files/UNICEF_Annual_Report_2015_En.pdf.

    Google Scholar 

  4. National AIDS Resource Center. HIV/AIDS Estimates and Projections in Ethiopia, 2011–2016; 2011. p. 1–2. available from http://fitun.etharc.org/resources/finish/53-hiv-aids-estimates-and-projections/327-hiv-aids-estimates-and-projections-in-ethiopia-2011-2016.

    Google Scholar 

  5. Koye DN, Ayele TA, Zeleke BM. Predictors of mortality among children on antiretroviral therapy at a referral hospital, Northwest Ethiopia: a retrospective follow up study. BMC Pediatrics. 2012;12:161.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Federal Ministry of Health of Ethiopia: Guidelines for comprehensive HIV prevention, care, and treatment. 2017. Available at https://www.childrenandaids.org/sites/default/files/201805/Ethiopia_Nat%20Guidelines%20ART_2017.pdf.

  7. UNAIDS: Ethiopia HIV Epidemic 2014. available from http://www.unaidsrstesa.org/wp-content/uploads/2015/05/UNAids-Profile-Ethiopia.pdf.

    Google Scholar 

  8. Government of Ethiopia Central Statistics: Demographic and health survey key indicators. 2016. Available at: https://www.usaid.gov/sites/default/files/documents/1860/Ethiopia%20DHS%202016%20KIR%20-%20Final%2010-17-2016.pdf.

    Google Scholar 

  9. Gebremedhin A, Gebremariam S, Haile F, Weldearegawi B, Decotelli C. Predictors of mortality among HIV infected children on anti-retroviral therapy in Mekelle hospital, Northern Ethiopia: a retrospective cohort study. BMC Public Health. 2013;13:1047.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Ebissa G, Deyessa N, Biadgilign S. Predictors of early mortality in a cohort of HIV-infected children receiving high active antiretroviral treatment in public hospitals in Ethiopia. AIDS Care. 2015;27(6):723–30.

    Article  PubMed  Google Scholar 

  11. Shimelash B. Assessment of the effect of malnutrition on survival of HIV infected children after initiation of antiretroviral treatment in Wolaita zone health facilities, SNNPR, Ethiopia: AAU; 2014.

  12. Taye B, Shiferaw S, Enquselassie F. The impact of malnutrition in survival of HIV infected children after initiation of antiretroviral treatment (ART). Ethiop Med J. 2010;48(1):1–10.

    PubMed  Google Scholar 

  13. World Health Organization. Antiretroviral therapy of HIV infection in infants and children: towards universal access: recommendations for a public health approach-2010 revision: World Health Organization; 2010.

  14. World Health Organization: WHO Child Growth Standards. 2006. Available at http://www.who.int/childgrowth/standards/Technical_report.pdf.

    Google Scholar 

  15. Turck D, Michaelsen KF, Shamir R, Braegger C, Campoy C, Colomb V, Decsi T, Domellöf M, Fewtrell M, Kolacek S. World health organization 2006 child growth standards and 2007 growth reference charts: a discussion paper by the committee on nutrition of the European society for pediatric gastroenterology, hepatology, and nutrition. J Pediatr Gastroenterol Nutr. 2013;57(2):258–64.

    Article  PubMed  Google Scholar 

  16. Federal Ministry of Health of Ethiopia: Guidelines for Paediatric HIV/AIDS Care and Treatment in Ethiopia 2008. available from https://www.ilo.org/wcmsp5/groups/public/---ed_protect/---protrav/---ilo_aids/documents/legaldocument/wcms_125387.pdf.

    Google Scholar 

  17. Sidamo N, Debere M, Enderis B, Abyu D. Incidence and predictors of mortality among children on anti-retroviral therapy in public health facilities of Arba Minch town, Gamo Gofa zone, southern Ethiopia; retrospective cohort study. Clin Mother Child Health. 2017;14:3.

    Article  Google Scholar 

  18. Anigilaje EA, Aderibigbe SA. Mortality in a cohort of HIV-infected children: a 12-month outcome of antiretroviral therapy in Makurdi, Nigeria. Indian Pediatr. 2018;2018:6409134.

  19. Jha UM, Dhingra N, Raj Y, Rewari BB, Jeyaseelan L, Harvey P, Chavan L, Saggurti N, Reddy DCS. Survival of children living with human immunodeficiency virus onantiretroviral therapy in Andhra Pradesh, India. Indian Pediatrics. 2018;55(4):301–5.

  20. Mulugeta A, Assefa H, Tewelde T, Dube L. Determinants of survival among HIV positive children on antiretroviral therapy in public hospitals, Addis Ababa, Ethiopia. Qual Prim Care. 2017;25(4):235–41.

    Google Scholar 

  21. Arage G, Assefa M, Worku T. Survival rate of HIV-infected children after initiation of the antiretroviral therapy and its predictors in Ethiopia: A facility-based retrospective cohort, vol. 7; 2019. p. 2050312119838957.

    Google Scholar 

  22. Wamalwa DC, Obimbo EM, Farquhar C, Richardson BA, Mbori-Ngacha DA, Inwani I, Benki-Nugent S, John-Stewart G. Predictors of mortality in HIV-1 infected children on antiretroviral therapy in Kenya: a prospective cohort. BMC Pediatr. 2010;10:33.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Adem AK, Alem D, Girmatsion F. Factors affecting survival of HIV positive children taking antiretroviral therapy at Adama Referral Hospital and Medical College, Ethiopia. J AIDS Clin Res. 2014;5(3).

  24. Mutanga JN, Mutembo S, Ezeamama AE, Song X, Fubisha RC, Mutesu-Kapembwa K, Sialondwe D, Simuchembu B, Chinyonga J, Thuma PE, et al. Long-term survival outcomes of HIV infected children receiving antiretroviral therapy: an observational study from Zambia (2003-2015). BMC Public Health. 2019;19(1):115.

    Article  PubMed  PubMed Central  Google Scholar 

  25. Kabue MM, Buck WC, Wanless SR, Cox CM, McCollum ED, Caviness AC, Ahmed S, Kim MH, Thahane L, Devlin A, et al. Mortality and clinical outcomes in HIV-infected children on antiretroviral therapy in Malawi, Lesotho, and Swaziland. Pediatrics. 2012;130(3):e591–9.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Zanoni BC, Phungula T, Zanoni HM, France H, Feeney ME. Risk factors associated with increased mortality among HIV infected children initiating antiretroviral therapy (ART) in South Africa. PLoS One. 2011;6(7):e22706.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Sutcliffe CG, van Dijk JH, Munsanje B, Hamangaba F, Siniwymaanzi P, Thuma PE, Moss WJ. Risk factors for pre-treatment mortality among HIV-infected children in rural Zambia: a cohort study. PLoS One. 2011;6(12):e29294.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Mwiru RS, Spiegelman D, Duggan C, Seage GR 3rd, Semu H, Chalamilla G, Kisenge R, Fawzi WW. Nutritional status and other baseline predictors of mortality among HIV-infected children initiating antiretroviral therapy in Tanzania. J Int Assoc Providers AIDS Care. 2015;14(2):172–9.

    Article  Google Scholar 

  29. Sullivan PS, Hanson DL, Chu SY, Jones JL, Ward JW. Epidemiology of anemia in human immunodeficiency virus (HIV)-infected persons: results from the multistate adult and adolescent spectrum of HIV disease surveillance project. Blood. 1998;91(1):301–8.

    Article  CAS  PubMed  Google Scholar 

  30. Mocroft A, Kirk O, Barton SE, Dietrich M, Proenca R, Colebunders R, Pradier C, Ledergerber B, Lundgren JD. Anaemia is an independent predictive marker for clinical prognosis in HIV-infected patients from across Europe. AIDS. 1999;13(8):943–50.

    Article  CAS  PubMed  Google Scholar 

  31. Dworkin MS, Adams MR, Cohn DL, Davidson AJ, Buskin S, Horwitch C, Morse A, Sackoff J, Thompson M, Wotring L. Factors that complicate the treatment of tuberculosis in HIV-infected patients. JAIDS J Acquir Immune Defic Syndr. 2005;39(4):464–70.

    Article  CAS  PubMed  Google Scholar 

  32. Yee D, Valiquette C, Pelletier M, Parisien I, Rocher I, Menzies D. Incidence of serious side effects from first-line anti-tuberculosis drugs among patients treated for active tuberculosis. Am J Respir Crit Care Med. 2003;167(11):1472–7.

    Article  PubMed  Google Scholar 

  33. Cardoso CA, Pinto JA, Candiani TM, Carvalho IR, Linhares RM, Goulart EM. The impact of highly active antiretroviral therapy on the survival of vertically HIV-infected children and adolescents in Belo Horizonte, Brazil. Memorias do Instituto Oswaldo Cruz. 2012;107(4):532–8.

    Article  CAS  PubMed  Google Scholar 

  34. Nugent J, Edmonds A, Lusiama J, Thompson D, Behets F, Care PH, Group T. Predicting mortality in HIV-infected children initiating highly active antiretroviral therapy in a resource-deprived setting. Pediatr Infect Dis J. 2014;33(11):1148–55.

    Article  PubMed  Google Scholar 

  35. Shahrin L, Leung DT, Matin N, Pervez MM, Azim T, Bardhan PK, Heffelfinger JD, Chisti MJ. Characteristics and predictors of death among hospitalized HIV-infected patients in a low HIV prevalence country: Bangladesh. PLoS One. 2014;9(12):e113095.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Moy FS, Fahey P, Nik Yusoff NK, Razali KA, Nallusamy R. Outcomes of human immunodeficiency virus-infected children after anti-retroviral therapy in Malaysia. J Paediatr Child Health. 2015;51(2):204–8.

    Article  PubMed  Google Scholar 

  37. Buck WC, Olson D, Kabue MM, Ahmed S, Nchama LK, Munthali A, Hosseinipour MC, Kazembe PN. Risk factors for mortality in Malawian children with human immunodeficiency virus and tuberculosis co-infection. Int J Tuberc Lung Dis. 2013;17(11):1389–95.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Njom Nlend AE, Loussikila AB. Predictors of mortality among HIV-infected children receiving highly active antiretroviral therapy. Med Mal Infect. 2017;47(1):32–7.

    Article  CAS  PubMed  Google Scholar 

  39. Hailemariam S, Bune GT, Ayele HT. Malnutrition: prevalence and its associated factors in people living with HIV/AIDS, in Dilla University referral hospital. Arch Public Health. 2013;71(1):13.

    Article  PubMed  PubMed Central  Google Scholar 

  40. Federal Ministry of Health of Ethiopia. Ministry of Health National Nutrition and HIV/AIDS Implementation Reference Manual; 2008. p. 3. Available at: https://www.fantaproject.org/sites/default/files/resources/Ethiopia-HIV-Nutrition-Guidelines-2008.pdf.

    Google Scholar 

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Acknowledgments

We want to extend our deepest gratitude to the University of Gondar, College of Medicine and Health Sciences, for this research project’s financial funding. We would also express our special thanks to both data collectors and supervisors.

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Authors and Affiliations

Authors

Contributions

AA: conception of the research idea, design, data collection, analysis, interpretation, and manuscript write-up. EHE, MMK, PP, GDK, FW, and SE: data collection, analysis and interpretation, and manuscript write-up. GA, ZWB, DBK, GG, BT, YTH, and MWM: reanalysis, reinterpretation, and reviewing the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Animut Alebel.

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Ethics approval and consent to participate

Human Ethics was written from the School of Nursing ethics review committee, College of Medicine and Health Sciences, University of Gondar (Ref.No: N/D/535/07/2009) The ethics committee formally waived the need of formal written consent as the study was based on secondary data. A supportive letter was written from each study hospital’s administration. As this was a historical study, informed consent from individual patients was not requested or required. Physical or psychological harms to the study subjects were very minimal. The collected data were none-identifiable during data management and analysis. Finally, computer data were locked by personal secured password.

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The authors have declared that they have no competing interests.

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Alebel, A., Engeda, E.H., Kelkay, M.M. et al. Mortality rate among HIV-positive children on ART in Northwest Ethiopia: a historical cohort study. BMC Public Health 20, 1303 (2020). https://doi.org/10.1186/s12889-020-09418-6

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