- Research article
- Open Access
Risk factors of hepatitis B virus surface antigen carriage and serological profile of HBsAg carriers in Lomé Togo, 2016
BMC Public Health volume 19, Article number: 32 (2019)
In Togo, the prevalence of Hepatitis B Virus Surface Antigen (HBsAg) among young people aged 15–24 years was estimated at 16.4% in 2010; however, risk factors for HBsAg carriage are poorly documented. We sought to identify risk factors for HBsAg carriage and the serological profile of HBsAg carriers in Lomé (capital city of Togo).
We conducted a case control study from October 2016 to March 2017 in Lomé. Cases and controls were randomly selected from a database of Institut National d’Hygiène (INH) of Lomé during a free screening campaign for hepatitis B. We calculated means, frequencies, proportions, odds ratios (OR), and 95% confidence interval (CI) and performed logistic regression.
We included 83 confirmed cases and 249 controls. The median age was 31 years among cases and 30 years among the controls. The sex ratios (M/F) were 11/6 among cases and 4/3 for the controls. The independent risk factors for HBsAg carriage were the awareness of hepatitis B serological status (OR = 3.56, 95% CI [1.80–7.04]) and Kabyè-tem ethnic group (OR = 3.56, 95% CI [1.98–6.39]). Among HBsAg carriers, 13.3% were at the viral replication stage (all of whom were between 30 and 45 years of age) and 1.2% were at the acute stage of the disease. The prevalence of co-infection with hepatitis B and C was 4.80%. All co-infections were in women aged 24–28 years.
The Kabyè-tem ethnic group is at risk of HBsAg carriage in Lomé. Of note, most HBsAg carriers in this ethnic group are aware of their HBsAg serological status. Furthermore, the prevalence of Hepatitis among adults of reproductive age is high and is cause for concern. We therefore recommend screening and vaccination campaigns at subsidized prices among people aged 30 years and older.
Hepatitis B is an inflammatory disease of the liver characterized by hepatocellular necrosis and caused by a virus belonging to the Hepdnaviridae family . Hepatitis B is a major public health concern across the globe. The World Health Organization (WHO) estimated that approximately 240 million people are infected with the Hepatitis B Virus (HBV) worldwide  but only 11% of those who live with viral hepatitis are aware of their serologic status . These individuals are at risk of severe illness and death as a result of cirrhosis or hepatocellular carcinoma (HCC). The viral hepatitis pandemic takes a heavy toll on lives, communities and health systems. In 2013, viral hepatitis was the seventh highest cause of mortality globally. It is responsible for an estimated 1.4 million deaths per year from acute infection and hepatitis-related liver cancer and cirrhosis a toll comparable to that of HIV and tuberculosis. Of those deaths, approximately 47% are attributable to HBV [4, 5]. The majority of hepatitis B infections are in Africa where the prevalence is estimated to be 7.2% (6.2–8.2) .
The virus is typically acquired via percutaneous or mucosal exposure to infected blood or other body fluids . Because the virus can also be transmitted from mother to child during delivery and through unprotected sexual intercourse, infection of sexually active adults of reproductive age is particularly concerning. Acute HBV infection is characterized by the presence of hepatitis B surface antigen (HBsAg) and Immunoglobulin M (IgM) against Hepatitis B virus core antigen (anti-HBc IgM). During the initial phase of the infection, which is characterized by a high level of viral replication, the patients are carriers of the Hepatitis B viral antigen (HBeAg). The presence of HBeAg indicates that the subject is highly contagious. Antibodies against HBsAg (anti-HBs antibodies) appear a few weeks later, following which HBsAg is lost. Chronic infection is characterized by the persistence of HBsAg beyond 6 months . The persistence of HBsAg is an important risk factor for development of chronic hepatitis and liver cancer.
A previous study estimated the prevalence of this disease in Togo to be 16.4% among young people (aged 15–24 years) in 2010  it varied significantly with gender, marital status, place and region of residence. In that study, the prevalence was 19.2% among males and 13.9% among females. A study conducted in 2011 at University Hospital of Campus in Lomé found a prevalence of 19.08% with 25% in males and 14.8% in females . In 2015, the estimated prevalence of HBsAg was 4.6% among first-year University students at the University of Lomé . These prevalence figures are higher than the average recorded for the West African sub region highlighting the importance of this public health concern in Togo. Public health authorities have been progressively implementing measures to prevent hepatitis B infection since 2008, as evidenced by the integration of hepatitis B vaccination into the Expanded Program on Immunization (EPI) [12, 13]. A workshop on hepatitis B was organised in Lomé in November 2016. On this occasion, Institut National d’Hygiène of Lomé (INH) organized a free screening campaign during which 476 people were enrolled. Blood samples were collected from participants and tested for the presence of HbsAg with Enzyme Linked Immuno Sorbent Assay (ELISA). Samples which tested positive for HBsAg were further analyzed for HBeAg, anti-HBc IgM antibodies, and antibodies against Hepatitis C virus.
A database was established at the end of this campaign. In this study we present an analysis of that database, focusing on risk factors for HbsAg carriage and serological profiles of people with HbsAg, which are still poorly documented in Togo. The objectives of this study were to identify factors associated with carriage of HBsAg and to describe the serological profile of people with HBsAg in Lomé.
We conducted a case-control study at the INH of Lomé from October 2016 to March 2017. The study population consisted of individuals residing in Lomé who participated in a free screening campaign for HBsAg in October 2016. Information about the campaign was broadcasted on public radio and television to maximize the catchment of the campaign throughout the city of Lomé. A database containing serology data from the participants was built during this screening campaign. All participants were educated on the goals of the study and gave a written consent to participate. Prior to the study we obtained the approval of the INH internal ethics council.
Terms and definitions
Case: Anyone who participated in the free HBsAg screening test at INH in October 2016 and tested positive on the ELISA HBsAg assay.
Control: Anyone who participated in the free HBsAg screening test at INH in October 2016 and tested negative on the ELISA assay HBsAg.
Active viral replication: Simultaneous presence of HBsAg and HBeAg.
Acute phase of hepatitis B: Simultaneous presence of HBsAg and anti-HBc IgM antibodies.
Sampling and data collection
For a risk of 5%, a power of 80%, a case and control ratio of 1/3, a proportion of controls and cases with exposures of 40 and 57% respectively and an odd ratio of 2.1 we determined the minimal sample size to be 77 cases and 249 controls. We randomly selected controls and cases from records in database described in the study details. There were no exclusion criteria for the study. We gathered participant information through a questionnaire. In addition, we examined the laboratory register or the filled questionnaires to complete missing data and to delete duplications.
We were interested in socio-demographic information such as age, sex, ethnic group, level of education, marital status, and place of residence. We also considered knowledge about hepatitis B transmission route and means of prevention. These variables are of interest because they may correlate with behavioural measures to avoid acquisition of the virus. In addition, we documented possible avenues of exposure such as prior blood transfusion, surgery, piercing, vaccination, dental care or surgery, hospitalization, and neighbours suffering of hepatitis B. Finally, we tracked serological markers: HBsAg, HBeAg, anti-HBc IgM antibody, and hepatitis C markers.
All blood samples were analysed for the presence of the HBsAg by means of the ELISA with Monolisa Kit from Biorad made in France. Samples which tested positive for HBsAg were further analysed for the presence of the HBeAg and anti-HBc IgM antibody by Electro-chemiluminescence immuno-assay (ECLIA) on Cobas machine from Roche and for the presence of Hepatitis C virus markers by ELISA with Monolisa Kit from Biorad.
We completed the missing data, corrected the duplicates of the database and performed analyses using Epi Info 7. We calculated means, frequencies, proportions and measured associations by calculating the odds ratios (OR). We compared proportions by Fisher exact or Chi-square tests with a significance threshold of p ≤ 0.05.
Risk factors that were associated with HBsAg with p < 0.20 in bivariate analysis were introduced into a multivariable logistic regression model.
The explanatory variables were: previous history of vaccination, blood transfusion, hospitalization, surgery, dental care or surgery, ethnic group, sex, marital status, knowledge of HBV status, neighbour suffering of hepatitis B. The explained variable was the carriage of the HBsAg. We checked interactions of variables.
Subjects enrolled in the free screening gave a written consent. Prior to the use of the screening database, we obtained clearance from the internal ethics council of INH of Lomé.
Cases and controls characteristics
We included 83 cases and 249 controls in the study. Cases and controls had median ages of 31 years old (IQR 26–39 years) and 30 (IQR 23–41 years) respectively. Females represented 36.14% of cases and 43.5% of controls. Sex ratio (M/F) was 11/6 for cases and 4/3 for controls.
The characteristics of cases and controls are presented in Table 1.
Tables 2, 3 and 4 show the factors associated with the carriage of HBsAg in Lomé in bivariate analysis. Carriage of HBsAg was associated with ethnic group, age group, marital status, knowledge of the mode of contamination, and the knowledge of the hepatitis B serological status. No statistically significant association could be demonstrated between the carriage of HBsAg and previous hospitalization, surgery, dental care, tattooing, a neighbour suffering of Hepatitis B or vaccination.
Being of the Kabyè-tem ethnic group and knowing its HBV serological status were demonstrated as independent risk factors for carrying HBsAg as shown in Table 5. Vaccination did not appear to have a statistically significant protective effect.
Serological profile of HBsAg carriers
As shown in Table 6, 13.3% of HBsAg carriers were at the stage of active viral replication (i.e. carry HBeAg), and 1.2% were in the acute phase or in activation (i.e. carry the anti-HBc IgM antibody), while 4.8% had markers of hepatitis C virus infection. The median age of the hepatitis B cases which were categorized as being in the stage of viral replication was 32.27 years (IQR 25–40 years), the sex ratio (M / F) =3/4. All patients in this category belong to the Kabyè-tem ethnic group. Additional features of persons in viral replication are presented in Table 7. HBV and HCV co-infected people were all female and aged 24–28 years and belong to Kabyè-tem ethnic group. Participants in this subgroup were also unaware of their status and did not know the routes of transmission or methods for prevention of HBV.
The Kabyè-tem ethnic group membership and knowledge of the serological status before the test, were identified as two independent risk factors associated with HBsAg carriage. Of the infected persons, 13.3% were still infectious, and these were all women from the Kabyè-tem ethnic group. Our findings corroborate the results of a previous study that observed that the regions of highest HBsAg seroprevalence among 15–24 years old individuals were the central region (27.7%) and the Kara region (23.1%), which are inhabited by the Kabyè-tem . This ethnic group is the second largest in Togo. Although Kabyè-tem primarily live in the central and Kara regions, many have moved to reside in Lomé and were thus included in this study. To our knowledge, there has not been a study comparing the socio-economic conditions of members of the Kabyè-tem ethnicity relative to other ethnicities. That belonging to the Kabyè-tem group is an independent risk factor for HBsAg carriage could be explained by socio-cultural or genetic factors. Several studies [14, 15] have described the influence of genetic factors on HBV infection and on the elimination of HBsAg.
The fact that knowledge of serological status was shown to be an independent risk factor indicates that most cases were aware of their serological status compared to controls and that screening for HBsAg was not routinely done in Togolese populations. The cases were probably diagnosed with hepatitis B during clinical manifestations prior to our survey. Statistically significant associations could not be identified between HBsAg carriage and the male gender, transfusion, hospitalization, surgery, tattoos and piercing, or having a parent suffering from hepatitis B. Similar observations were made in a previous study conducted in Nigeria in 2011  and another conducted in Ghana in 2013 . This may be related to the fact that this information was obtained on the basis of statements from study participants, they were not likely willing to disclose this information because of stigma. Also, the sample size was too small for identification of certain risk factors such as transfusion, prior hospitalization, or dental surgery. In addition, it is possible that the fact that blood transfusions, hospitalization, and nursing practices are becoming increasingly safe  minimized the rate of hepatitis acquisition via iatrogenic routes.
In the hospital-based Lomé study and the national survey among people aged 15 to 24 years there was a statistically significant difference in the prevalence of HBsAg between the two genders [9, 10]. This discrepancy may be due to our smaller sample size. We also found that vaccination did not have a statistically significant protective effect, this possibly reflects a poor adherence to vaccination schedules.
Of HBsAg carriers, 13.3% also carried HbeAg and were therefore still contagious. Importantly, this population is likely sexually active (median age 28 years) and therefore at risk of transmitting the virus to uninfected individuals . A similar prevalence (13.7%) was found in a study carried out among PLHIV on ART in Lomé in 2011 , indicating that the odds of sexual transmission in Lomé have remained high for some time.
The prevalence of hepatitis B and C co-infection was 4.8%, which was higher than the 3.9% value previously reported among untreated HIV patients in Tanzania during 2006 . It is also much higher than the 0.6% previously reported in Ghana among pregnant women in the Ashanti region . It therefore appears that co-infection is a significant issue in Togo.
Three major limitations were identified. The first limitation is that our study did not incorporate risk factors such as unsafe sexual behaviour and type of breastfeeding. The second was the fact that the serological diagnosis of the hepatitis B we used in our study may have not detected occult hepatitis whose prevalence is approximately 5% among HBsAg negative individuals [21, 22]. Our study may therefore have underestimated the association with risk factors. The third was that the pre-existing database from which we used unfortunately, did not include information regarding sexual practices and intravenous drug use by the participants; these important risk factors will be addressed in future studies. Despite these limitations, analysis of this database allowed us to identify factors associated with HBsAg carriage and the serological profile of HBsAg carriers in Lomé in 2016.
Our study showed that Kabyè-tem ethnic group and awareness of hepatitis B serological status are independent risk factors for HBsAg carriage in Lomé. A proportion of 13% of HBsAg carriers in Lomé, were in the contagious phase, implying that the risk of transmission of this disease to sexual partners and close neighbours (parents, nurses, domestic workers) remains high in Togo. Education campaigns to encourage screening and subsidization of the vaccination especially among the Kabyè-tem community (particularly women) will likely promote effective control of hepatitis B in Togo. The Expanded Program on Immunization should be reviewed in order to incorporate introduction of Hepatitis B vaccine earlier during the first hours of life to minimize the risks of mother to child transmission.
Centre Hospitalier Universitaire
Enzyme linked fluorescent assay
Enzyme Linked Immuno Sorbent Assay
Expanded Program on Immunization
e Antigen of hepatitis B virus
Surface antigen of hepatitis B virus
Hepatitis B virus
Institut National d’Hygiène
Polymerase chain reaction
Person living with HIV
World Health Organisation
WHO. Guidelines Approved by the Guidelines Review Committee. In: Guidelines for the Prevention, Care and Treatment of Persons with Chronic Hepatitis B Infection. Geneva: World Health Organization; 2015.
World Health Organization. Global Health Sector Strategies on Viral Hepatitis 2016-2021. 2016. p. 13. Avialable on http://apps.who.int/gb/ebwha/pdf_files/WHA69/A69_32-en.pdf?ua=1.
Finding the missing millions the world hepatitis alliance’s 2018 – 2020 strategic plan available on http://www.worldhepatitisalliance.org.
Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, Abraham J, Adair T, Aggarwal R, Ahn SY, et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the global burden of disease study 2010. Lancet. 2012;380(9859):2095–128.
IHME. Global burden of disease and WHO/UNAIDS estimates. In: Global Burden of Disease; 2017.
The Polaris Observatory Collaborators. Global prevalence, treatment, and prevention of hepatitis B virus infection in 2016: a modelling study. Lancet Gastroenterol Hepatol 2018, Published Online March 26, 2018. https://doi.org/10.1016/S2468-1253(18)30056-6.
WHO. Weekly epidemiological record. In: World Health Organization, vol. vol. 40; 2009. p. 405–20.
Chen L, Liu F, Fan X, Gao J, Chen N, Wong T, Wu J, Wen SW. Detection of hepatitis B surface antigen, hepatitis B core antigen, and hepatitis B virus DNA in parotid tissues. Int J Infect Dis. 2009;13(1):20–3.
Banla AKGK, Halatoko WA, Layibo Y, Akolly K, Maman I, Tamekloe TA, Feteke L, Vovor A, Pitche P. Prevalence of the surface antigen of hepatitis B virus among youth aged 15 to 24 in TOGO in 2010. J Infec Dis Ther. 2015;3:1–5.
Kolou M, Katawa G, Salou M, Gozo-Akakpo KS, Dossim S, Kwarteng A, Prince-David M. High prevalence of hepatitis B virus infection in the age range of 20-39 years old individuals in Lome. Open Virol J. 2017;11:1–7.
Ekouevi D, Thomas A, Sewu D, Lawson-Ananissoh L, Bk T, Salou M, Ketoh G, David M, Coffie P, Redah D. Prevalence of Hepatitis B among Students from the University of Lomé, Togo in 2015, vol. vol. 07; 2017.
Ministère de la Santé du Togo. Programme Elargi de Vaccination du Togo, Rapport d’activités de l’année 2009. 2010 p. 7
Breakwell L, Tevi-Benissan C, Childs L, Mihigo R, Tohme R: The status of hepatitis B control in the African region. 2017(1937–8688 (Electronic)).
Espert L1, Degols G, Gongora C, Blondel D, Williams BR, Silverman RH, Mechti N. SG20, A new interferon-induced RNase specific for single-stranded RNA, defines an alternative antiviral pathway against RNA genomic viruses. J Biol Chem. 2003;278(18):16151–8.
Hennig BJ, Hall AJ. Host genetic factors in hepatitis B infection, liver cancer and vaccination response: a review with a focus on Africa. Sci Total Environ. 2012;423:202–9.
Olalekan A. Prevalence and risk factors for hepatitis B and C among sexually active undergraduates in southwestern Nigeria. Ann Trop Med Public Health. 2015;8(6):235–40.
Ephraim R, Donko I, Sakyi SA, Ampong J, Agbodjakey H. Seroprevalence and risk factors of hepatitis B and hepatitis C infections among pregnant women in the Asante Akim north municipality of the Ashanti region, Ghana; a cross sectional study. Afr Health Sci. 2015;15(3):709–13.
Lok AS, McMahon BJ. Chronic hepatitis B: update 2009. Hepatology. 2009;50(3):661–2.
Patassi A A, Kotosso A, Salou M, Bawe LD, Krivine A, Ephrem M, et al. Prevalence des marqueurs du virus de l’hepatite B chez les patients infectes par le VIH et traites par anti-retroviraux dans un pays a ressources limitees. J Rech Sci Univ Lomé. 2015;17(3):685–92.
Nagu TJ, Bakari M, Matee M. Hepatitis A, B and C viral co-infections among HIV-infected adults presenting for care and treatment at Muhimbili National Hospital in Dar Es Salaam. Tanzania BMC public health. 2008;8:416.
Gachara G, Magoro T, Mavhandu L, Lum E, Kimbi HK, Ndip RN, Bessong PO. Characterization of occult hepatitis B virus infection among HIV positive patients in Cameroon. AIDS Res Ther. 2017;14(1):11.
Hudu SA, Harmal NS, Saeed MI, Alshrari AS, Malik YA, Niazlin MT, Hassan R, Sekawi Z. Molecular and serological detection of occult hepatitis B virus among healthy hepatitis B surface antigen-negative blood donors in Malaysia. Afr Health Sci. 2016;16(3):677–83.
We would like to thank the Institut National d’Hygiène de Lomé and the West African Field Epidemiology Programme for their support.
The content of the manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the Institut National d’Hygiène.
The INH is Togolese the national institute for biological confirmation of epidemic-prone diseases. Consistent with its mission to inform public health efforts in the republic of Togo, INH funded the free campaign, and provided materials as well as reagents for sample collection and biological analyses. The data analysis was completed without any additional funding.
Availability of data and materials
Extracted data are with the authors (Wemboo Afiwa Halatoko, Adjaho Koba and Yao Akolly) and available for sharing upon request.
Ethics approval and consent to participate
We used an existing database build with the approval of the INH internal ethics council. Prior to the exploitation of the database, we obtained an authorization from the Institut National d’Hygiène of Lomé. People who participated to the free campaign gave a written consent.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Halatoko, W.A., Patassi, A., Yanogo, P. et al. Risk factors of hepatitis B virus surface antigen carriage and serological profile of HBsAg carriers in Lomé Togo, 2016. BMC Public Health 19, 32 (2019). https://doi.org/10.1186/s12889-018-6320-x
- HBs antigen
- Risk factors
- Serological profile