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Use of ‘eradication’ in HIV cure-related research: a public health debate
© The Author(s). 2018
- Received: 12 October 2017
- Accepted: 1 February 2018
- Published: 13 February 2018
The landscape of Human Immunodeficiency Virus (HIV) research has changed drastically over the past three decades. With the remarkable success of antiretroviral treatment (ART) in decreasing AIDS-related mortality, some researchers have shifted their HIV research focus from treatment to cure research. The HIV cure research community often uses the term eradication to describe the science, and talks about eradicating the virus from the body. In public discourse, the term eradication could be conflated with disease eradication at the population level. In this paper, we call for a reframing of HIV cure research as control, as it is a more accurate descriptor and achievable goal in the foreseeable future.
The properties of HIV are discordant with eradicability standards at both the individual level (as a clinical concept), and at the population level (as a public health concept). At the individual level, true eradication would necessitate absolute elimination of all latent HIV reservoirs from the body. Current HIV cure-related research strategies have proven unsuccessful at accurately quantifying, let alone eliminating these reservoirs. At the population level, eradication implies the permanent global reduction of HIV to zero new cases and to zero risk for future cases. Given the absence of an efficacious HIV vaccine and the impracticality and unethicality of eliminating animal reservoirs, global eradication of HIV is highly implausible. From a public health perspective, HIV eradication remains an elusive goal.
The term ‘eradication’ is a misleading description of current HIV cure-related research. Instead, we call for the use of more realistic expressions such as ‘sustained virologic HIV suppression (or control)’ or ‘management of HIV persistence’ to describe HIV cure-related research. Using these terms reorients what HIV cure science can potentially achieve in the near future and avoids creating unrealistic expectations, particularly among the millions of people globally who live with HIV.
- HIV cure
- Public health
An estimated 36.7 (30.8–42.9) million people live with HIV worldwide, and approximately 1.8 (1.6–2.1) million new infections occurred in 2016 . Global scale-up of HIV antiretroviral treatment (ART) increased the life expectancy of people living with HIV, contributing to a 48% reduction in AIDS-related mortality between 2005 and 2016 [1, 2]. However, ART does not remove replicative HIV from the body and is not a cure . Barriers to an HIV cure include the persistence of quiescent HIV reservoirs that are not sensitive to or reached by ART nor visible to the immune system. The last decade has seen an increase in biomedical HIV cure research . The U.S. Food and Drug Administration (FDA) defines HIV cure research as: “any investigation that evaluates: 1) a therapeutic intervention or approach that controls or eliminates HIV infection to the point that no further medical interventions are needed to maintain health; and 2) preliminary scientific concepts that might ultimately lead to such a therapeutic intervention” . HIV cure research involves a variety of biomedical approaches (e.g. latency-reversing compounds; early and intensified HIV therapy; immune-based strategies and therapeutic vaccines; gene editing; stem cell transplants; or combined approaches) . Two main pathways to achieving sustained ART-free HIV suppression are under investigation: 1) removal of all replication-competent HIV reservoirs from the body (classic biomedical cure), and 2) control of viral rebound in the absence of ART without complete elimination of HIV (‘functional cure’) .
Eradication is a distinct concept at the individual level and at the population level. On an individual patient level, HIV cure would mean eradication of the virus from the body. HIV cure remains an aspirational goal for most people living with HIV. One person, Timothy Ray Brown, has been cured of HIV infection , but the science of HIV cure research has not progressed to the point where eradication of HIV reservoirs from the body (a cure) is possible. The primary intent of most HIV cure interventions remains long-term virologic suppression of HIV in the absence of ART. Most HIV cure research approaches under clinical investigation only have the capacity to reduce but not eliminate the reservoir size or augment the immune system’s ability to control HIV while off ART. On a population level and as a public health concept, eradication is defined as the permanent reduction to zero of the worldwide incidence of a disease . For example, this would mean the elimination of transmission and disease, as seen with smallpox eradication. From a public health perspective, eradication is inherently an incidence concept, not a cure concept. Moreover, HIV does not meet the criteria for disease eradicability set forth by the World Health Organization (WHO) and the International Task Force for Disease Eradication (ITFDE) [8, 9].
While the HIV cure research community uses the term eradication as a clinical concept, it could easily be misinterpreted as disease eradication at a population level in public discourse. Scientific groups such as the Collaborative HIV Eradication of Viral Reservoirs (CHERUB), the amfAR Research Consortium on HIV Eradication (ARCHE) and the Collaboratory of AIDS Researchers for Eradication (CARE) are dedicated to finding a clinical cure for HIV infection. The biomedical HIV cure research literature is also rich with examples of potential ‘eradication therapies’ for HIV infection [10–15]. The term eradication is used to galvanize interest and funding in the science. Elimination would be a better term to describe the complete disappearance of HIV from the body as an aspirational cure goal. Instead of eradication, we also suggest the use of expressions such as ‘long-term virologic suppression (or control) off therapy’, or ‘management of HIV persistence’ [6, 16]. Control of HIV makes much more sense as it is a well-documented concept and can be linked appropriately to viral replication [17, 18]. From a public health perspective, when the term eradication is used loosely, it can easily be conflated with HIV elimination or control on a global scale. The public should be informed that HIV cure research will not lead to disease eradication on a population level. Using the term ‘control’ on a clinical and population levels provides a reorientation of what HIV cure science can potentially achieve in the foreseeable future and avoids creating false hopes and expectations , particularly among the millions of people globally who live with HIV.
The term eradication borrows its origins from the Latin word eradicat, meaning “torn up by the roots” . In HIV cure-related research, the term eradication is used to denote the complete elimination of all replication-competent forms of the virus (eradicating or sterilizing cure) . Relatedly, language used to describe the science provides an opportunity to educate the community and potential volunteers regarding what can be accomplished to avoid the overestimation of possible medical benefits . Careful examination of HIV cure-related research terminology reveals the terms used are complex, and oftentimes considered only in narrow biomedical contexts in isolation from the social contexts of disease [17, 18]. HIV cure-related research carries hopes and misconceptions. Using language such as cure or eradication risks creating public misapprehension about incremental research developments . This paper builds on the literature interrogating the use of language in HIV cure-related research [17, 18, 21, 22] and on a rich history of questioning language used in the scientific literature in relation to HIV (e.g. for example, gay-related immune deficiency (GRID) was renamed acquired immune deficiency virus (AIDS) in 1982).
At the individual (clinical) level, the expression HIV control is more descriptive than HIV eradication
Anthony Fauci, Director of the National Institute of Allergies and Infectious Diseases (NIAID), stated: “the complete eradication or elimination of HIV from an HIV-infected individual would be extremely difficult to achieve. At worst, it would be impossible. At best, it would be achievable in a very small subset of the HIV-infected population” .
At the individual level, HIV control may be considered a more descriptive endpoint than eradication. Complete removal of all HIV remnants is an extremely high bar to achieve . The fundamental biology of integrated proviral HIV deoxyribonucleic acid (DNA) makes it nearly impossible to know if all inducible virus has been removed . Another obstacle to curing HIV is ongoing viral replication, even in the presence of ART . The suspected mechanism of this residual ongoing replication is direct cell-to-cell transmission, in which HIV passes from an infected cell to an adjoining uninfected cell . Low-level viral replication escapes the effect of ART and contributes to the long-term persistence of HIV .
Timothy Ray Brown, the Berlin patient, is the only person currently cured of HIV . His cure involved a set of circumstances that have yet to be replicated. Following a relapsed acute myeloid leukemia (AML), he received two stem cell transplants from a donor homozygous for the CCR5 delta32 deletion. His initial case was described as ‘long-term control of HIV’ . Mr. Brown remained without replication-competent provirus for over 10 years. However, a recent study found trace amounts of replication-incompetent HIV nucleic acid in his body (researchers did not exclude the possibility of false positive results) . Even with the complete elimination of replication-competent HIV, finite particles of HIV may persist in the body, excluding true viral eradication.
Other case histories are similarly illustrative of the complexity of the issue and the need for precise language. Even small HIV reservoirs do not guarantee infection control when off ART . A recent case study showed an adult treated during early (Fiebig stage I) HIV infection with prolonged HIV suppression, followed by viral rebound. In this study, HIV treatment in the earliest stage of HIV infection resulted in a small number of latently infected cells but did not eliminate replicative HIV reservoirs . The Mississippi child represents another unusual case of prolonged HIV suppression with small HIV reservoirs . The perinatally infected infant tested positive at birth for HIV and immediately received ART. Her therapy was interrupted against medical advice when she was between 18 and 23 months old. The Mississippi child remained with undetectable viremia for approximately 27 months, until HIV was detected in her blood at 46 months of age . Because she started ART very early, it is possible that her HIV reservoir size was negligible; however, the return of HIV after a prolonged period of quiescent virus is consistent with the HIV latency hypothesis . Moreover, two patients from Boston underwent allogeneic bone marrow transplants to treat lymphoma, resulting in a reduction of their replication-competent HIV reservoir size . After a prolonged period of ART-free suppression, their virus also rebounded .
The Mississippi child and the Boston patients are emblematic cases demonstating the limits of HIV reservoir size estimations. Current assays to measure residual replication-competent HIV are not sufficiently sensitive to rule-out incomplete clearance of HIV from blood or tissues [10, 13, 30]. While some HIV cure research modalities aim to reduce the size of the inducible HIV reservoir (e.g., latency-reversing agents or stem cell transplants), prolonged ART-free suppression would require a drastic decrease in the pool of latently infected cells. These cells are extremely rare, with an average frequency of about 1 cell per 106 resting CD4+ T cell lymphocytes . The current gold standard for measuring these replication-competent HIV reservoirs is the Quantitative Viral Outgrowth Assay (QVOA). Though sensitive, the QVOA underestimates the size of the latent reservoir because some of the proviruses are not induced even after several rounds of viral outgrowth activation . In the Mississippi child and the Boston patients, for example, virus inducibility from latent reservoirs could not be detected during aviremic intervals, yet subsequent viral rebounds showed the presence of reproducible virus below the limit of detection [10, 29, 33]. The QVOA could not measure all latent virus in tissue sanctuaries  or other reservoirs (e.g., those in the brain) . Biomedical HIV cure researchers posit that only one replication-competent HIV DNA strand is needed for a total eruption of viral replication. Thus, the terms “reservoir reduction” or “viral control” may be better aligned with strategies aimed at depleting the HIV reservoir while strengthening the immune system’s ability to fight reawakening virus .
Much like cancer, proviral HIV is a residual disease, and a cure is limited by the persistence of rare cells . Replication-competent reservoirs can exist in blood or tissue even when they cannot be measured. The axiom ‘absence of evidence is not evidence of absence’ is apt in this case. Due to the limited ability to measure latent HIV, any patient who controls HIV off treatment would still require indefinite clinical follow-up to monitor HIV reactivation [30, 37].
Moreover, most current HIV cure research strategies do not lead to a substantial reduction in the size of the HIV reservoir. For example, early ART initiation restricts, but does not eliminate HIV reservoirs [38, 39]. Latency-reversing agents have not yet shown to cause a notable reduction in the size of the replication-competent HIV reservoirs in clinical studies [40, 41]. Immunotherapeutic approaches are being investigated to facilitate viral clearance [42–44], but do not inherently result in HIV reservoir eradication, but rather the more rapid removal of reactivating cells. Scientists are also studying gene modification strategies, particularly those blocking HIV cell entry . Genetically-edited cells can be reinfused into patients but to date engrafted cells represent a minority of the cell population, and viral relapse is common following ART interruption . In turn, stem cell transplants involve risky procedures, such as ablative conditioning, and are only appropriate for a very small portion of people living with HIV who have an underlying disease indicating such an approach . To date, no HIV cure research strategy has been able to overcome the challenge of eliminating proviral HIV latency or conferring viral suppression off ART at an individual level.
HIV does not meet criteria for eradicability at the population level
On a global scale, eradication is often conflated with disease control, which refers to the reduction of disease burden to an acceptable level, without the expectation that the disease would disappear forever .
The term eradication in relationship to disease gained popularity at the beginning of the twentieth century, following the establishment of germ theory and the growth of public health policy focused on designing countermeasures (e.g, vaccines and antibiotics). In 1909, the Rockefeller Foundation sponsored the Sanitary Commission for the Eradication of Hookworm Disease, followed by a similar Yellow Fever Commission in 1915 . After the Second World War, eradication was endorsed by the newly created WHO, with eradication efforts against malaria, smallpox, yaws, poliomyelitis and guinea worm disease (dracunculiasis) . Smallpox was the first, and thus far only, disease to meet the true absolutist goal of eradication (1980), following an intense and deliberate worldwide effort to bring its incidence to zero . Smallpox eradication required an efficacious vaccine conferring lifelong immunity, no animal hosts or intermediate vectors, and high symptomaticity so the disease could be identified efficiently during eradication efforts. Smallpox also had a high profile in public health in the twentieth century, and there was tremendous political will to bring its global incidence down to zero .
As demonstrated with smallpox, the meaning of the word eradication in global public health is the permanent reduction to zero new cases, and zero risk of cases  – or incidence of infection caused by a pathogen [8, 47]. Control in this context means the reduction of disease incidence, prevalence, and/or morbidity or mortality to an acceptable level, with continuous measures to keep the infection under control . Elimination of infection means the reduction to zero of the incidence of disease with continuous efforts to prevent re-establishment of transmission . Disease extinction signifies that the pathogen no longer exists in nature or in the laboratory . By this definition, no infection meets the extinction criteria since stocks of smallpox virus remain in two laboratories in Atlanta, GA, USA, and Moscow, Russia .
The International Task Force for Disease Eradication (ITFDE) has established their own criteria for disease eradication. Examining over 90 diseases, the ITFDE determined that only six – dracunculiasis, poliomyelitis, mumps, rubella, lymphatic filariasis, and cysticercosis – were candidates for eradication with currently available biomedical interventions . WHO subsequently called for the elimination of leprosy, onchocerciasis, and Chagas diseases as significant public health problems . The criteria met by these diseases include: (1) easily diagnosed; (2) effective interventions (e.g., vaccines) exist to break the cycle of transmission; and (3) infection occurs only in humans (i.e., no animal reservoirs). Selecting a disease candidate for eradication is not only a question of scientific feasibility, but also a matter of sincere political commitment and funding [8, 9, 46]. HIV eradication on a global scale is extremely difficult, if not impossible, to achieve in the absence of a highly efficacious vaccine  and the permanent existence of simian reservoirs harboring immunodeficiency virus. It would be impractical to eliminate animal reservoirs, short of exterminating all non-human primates in the wild carrying potentially infectious virus. This would not only be environmentally unacceptable and unethical, but also at odds with principles of ‘one health’ and disease ecology . Therefore, doubts remain as to the eradicability potential of HIV/AIDS in humans on a global scale.
A public health perspective to HIV control
Eradication as an absolute goal may rest on an over-reliance on biomedical technologies that fail to account for the complexity of transmission . To reduce the worldwide burden of HIV, it is critical to adopt public health strategies that leverage both biomedical and social interventions [49, 50]. A purely biomedical, therapeutic, or curative approach to HIV control is reductionist, failing to account for the complexity of disease. In fact, an effective response to HIV should be informed by a prediction of where the next 1000 new infections are likely to emerge, taking into account the deeper, and more intractable social, economic, behavioral, structural, and political determinants of HIV . Disease control will rest on keeping up the “momentum for effective, complex, combination HIV prevention and treatment efforts over the long term” . Yet scale-up of voluntary medical male circumcision remains a challenge in several African countries . Additionally, we are far from reaching the 90–90-90 HIV treatment targets globally that would faciliate achieving epidemic control through reduced viral loads . An over-emphasis on therapeutic and curative biomedical technologies may myopically create solutions that are inadequate and misguided to control such a profoundly social disease . A cure for HIV would be a tremendous scientific achievement, but would unlikely lead to HIV elimination as the disease is entangled with many complex structural determinants. Over-excitement in the HIV cure endeavor may eclipse the myriad socio-structural factors that create vulnerability to HIV in the first place .
On a global level, it may be worth asking if HIV ‘eradication’ is a worthwhile endeavor, knowing that animal reservoirs exist and that zoonotic leaps may lead to recurrent outbreaks in humans much like those seen with Ebola . While eradication has been a useful end goal for smallpox, with HIV it may be an example of over-confidence in biomedical approaches. From a public health perspective, it may be time to scale back the HIV eradication endeavor to focus instead on HIV control.
In sum, we argued for replacing the word eradication in HIV cure science as it exists. On an individual level, complete elimination of replication-competent HIV is an extremely high bar to achieve. While the term eradication is meant as a clinical concept by the HIV cure research community, it could be conflated with disease eradication at the population level in public discourse. On a population level, HIV eradication is an elusive goal, since HIV does not meet the criteria for disease eradicability [8, 9]. Framing HIV cure research as control research may help us calibrate expectations of what is possible, at least in the short term . Eradication requires perfection, setting the bar high and engendering chronic disappointment. Additionally, focusing on HIV eradication could lead to premature abandonment of a worthwhile enterprise. Further empirical research is needed to determine how people living with HIV, and other stakeholders, perceive the word eradication when discussing HIV cure research. We advocate to use expressions such as ‘sustained virologic HIV suppression (or control) off treatment’  or ‘management of HIV persistence’  to describe HIV cure-related research. These are more realistic and relevant endpoints. The control paradigms at individual and population levels allow us to more aptly appreciate incremental achievements in scientific research .
We are grateful to Nelson Michael (Military HIV Research Program, Walter Reed Army Institute of Research) for insights provided during the conceptualization stage of this manuscript. We are also indebted to Laurie Sylla from the defeatHIV Community Advisory Board (CAB), Seattle, WA for helpful feedback. We are grateful to Jo Gerrard who provided editorial assistance for this manuscript.
Availability of data and materials
KD draft the initial version of this manuscript. SL, BL, LN, JG, BB, SG reviewed the manuscript for intellectual contents. All authors read and approved the final manuscript.
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Not applicable. This manuscript did not involve human participants, human data or human tissue.
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- UNAIDS. UNAIDS Data 2017 [Internet]. 2017. Available: http://www.unaids.org/sites/default/files/media_asset/20170720_Data_book_2017_en.pdf
- Marcus JL, Chao CR, Leyden WA, Xu L, Quesenberry CP, Klein DB, et al. Narrowing the gap in life expectancy between HIV-infected and HIV-uninfected individuals with access to Care. JAIDS. 2016;73:39–46.PubMedPubMed CentralGoogle Scholar
- Chun TW, Stuyver L, Mizell SB, Ehler LA, Mican JA, Baseler M, et al. Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy. Proc Natl Acad Sci U S A. 1997;94:13193–7. Available: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=24285&tool=pmcentrez&rendertype=abstract View ArticlePubMedPubMed CentralGoogle Scholar
- TAG. Research Toward a Cure Trials [Internet]. 2017. Available: http://www.treatmentactiongroup.org/cure/trials
- FDA. Backgrounder for FDA’s HIV Patient-Focused Drug Development and HIV Cure Research Public Meeting [Internet]. 2013. Available: http://www.fda.gov/downloads/ForIndustry/UserFees/PrescriptionDrugUserFee/UCM354549.pdf
- Fauci A. Sustained ART-free HIV remission: opportunities and obstacles. International AIDS Society 2017. Available: http://programme.ias2017.org/Search/Search?search=Fauci
- Allers K, Hütter G, Hofmann J, Loddenkemper C, Rieger K, Thiel E, et al. Evidence for the cure of HIV infection by CCR5Δ32/Δ32 stem cell transplantation. Blood. 2011;117:2791–9. https://doi.org/10.1182/blood-2010-09-309591.View ArticlePubMedGoogle Scholar
- Dowdle W. Principles of Disease Elimination and Eradication. In: MMWR Morb Mortal Wkly Rep [Internet]. 1999 [cited 18 Sep 2017] pp. 23–7. Available: https://www.cdc.gov/mmwr/preview/mmwrhtml/su48a7.htm
- CDC. Recommendations of the International Task Force for Disease Eradication. MMWR. 1993;42: 46 pages. Available: https://www.cdc.gov/mmwr/PDF/rr/rr4216.pdf
- Spivak AM, Planelles V. HIV-1 eradication: early trials (and tribulations). Trends Mol Med Elsevier Ltd. 2016;22:10–27. https://doi.org/10.1016/j.molmed.2015.11.004.View ArticleGoogle Scholar
- Margolis DM. Eradication Therapies for HIV Infection: Time to Begin Again. AIDS Res Hum Retroviruses. 2011;27(4):347–53. https://doi.org/10.1089/aid.2011.0017.View ArticlePubMedPubMed CentralGoogle Scholar
- Archin N, Sung J, Garrido C, Soriano-Sarabia N, Eradicating MD. HIV-1 infection: seeking to clear a persistent pathogen. Nat Rev Microbiol. 2014;12:750–64. https://doi.org/10.1002/dev.21214.Developmental.View ArticlePubMedPubMed CentralGoogle Scholar
- Martin AR, Siliciano RF. Progress Toward HIV eradication: case reports, current efforts, and the challenges associated with cure. Annu Rev Med. 2016;67:1–14. https://doi.org/10.1146/annurev-med-011514-023043.View ArticleGoogle Scholar
- Zhang J, Crumpacker C. Eradication of HIV and cure of AIDS, now and how? Front Immunol. 2013;4:337. https://doi.org/10.3389/fimmu.2013.00337.View ArticlePubMedPubMed CentralGoogle Scholar
- Tateishi H, Monde K, Anraku K, Koga R, Hayashi Y. A Clue to Unprecedented Strategy to HIV Eradication: “Lock-in and apoptosis.” Sci Rep. Springer US. 2017;7:8957. https://doi.org/10.1038/s41598-017-09129-w.Google Scholar
- Fauci A, Addressing HIV. Persistence: a novel approach involving α4β7 integrin. Strategies for an HIV Cure. 2016;Google Scholar
- Tucker J, Volberding P, Margolis D, Rennie S, Barré-Sinoussi F. Words matter: discussing research towards an HIV cure in research and clinical contexts. JAIDS J Acquir Immune Defic Syndr. 2014;67:110–1.View ArticleGoogle Scholar
- Rennie S, Siedner M, Tucker JD, Moodley K. The ethics of talking about “HIV cure”. BMC Med Ethics. 2015;16:18. https://doi.org/10.1186/s12910-015-0013-0.View ArticlePubMedPubMed CentralGoogle Scholar
- Dubé K, Henderson GE, Margolis DM. Framing expectations in early HIV cure research. Trends Microbiol. 2014;22:547–9. https://doi.org/10.1016/j.tim.2014.08.003.View ArticlePubMedPubMed CentralGoogle Scholar
- English Oxford Living Dictionaries. “Eradicate” [Internet]. 2017 [cited 18 Sep 2017]. Available: https://en.oxforddictionaries.com/definition/eradicate
- Nie J-B, Gilbertson A, Malcolm de R, Staunton C, van NA, Tucker JD, et al. Healing without waging war: beyond military metaphors in medicine and HIV cure research. Am J Bioeth. 2016;16:3–11. https://doi.org/10.1080/15265161.2016.1214305.View ArticlePubMedPubMed CentralGoogle Scholar
- Guta A, Newman PA. Of HIV, kings, and cures: troubling the apocryphal apothecary. Am J Bioeth. 2016;16:25–7. https://doi.org/10.1080/15265161.2016.1214313.View ArticlePubMedGoogle Scholar
- Hütter G. More on shift of HIV tropism in stem-cell transplantation with CCR5 delta32/delta32 mutation. N Engl J Med. 2014;371:2437–8.View ArticlePubMedGoogle Scholar
- Yukl S, Boritz E, Busch M, Bentsen C, Chun T-W, Douek D, et al. Challenges in detecting HIV persistence during potentially curative interventions: a study of the berlin patient. PLoS Pathog. 2013;9:e1003347. https://doi.org/10.1371/journal.ppat.1003347.View ArticlePubMedPubMed CentralGoogle Scholar
- Chun T-W, Justement JS, Murray D, Hallahan CW, Maenza J, Collier AC, et al. Rebound of plasma viremia following cessation of antiretroviral therapy despite profoundly low levels of HIV reservoir: implications for eradication. AIDS. 2010;24:2803–8. https://doi.org/10.1097/QAD.0b013e328340a239.View ArticlePubMedPubMed CentralGoogle Scholar
- Henrich TJ, Hatano H, Bacon O, Hogan LE, Rutishauser R, Hill A, et al. HIV-1 Persistence Following Extremely Early Initiation of Antiretroviral Therapy (ART) during Acute HIV-1 Infection: An Observational Study. PLoS Med. 2017;11: e1002417. doi:doi: https://doi.org/10.1371/journal.pmed.1002417
- Persaud D, Gay H, Ziemniak C, Chen YH, Piatak M, Chun T-W, et al. Absence of detectable HIV-1 viremia after treatment cessation in an infant. N Engl J Med. 2013;369:1828–35. https://doi.org/10.1056/NEJMoa1302976.View ArticlePubMedPubMed CentralGoogle Scholar
- Henrich T, Hatano H, Hill A, Bacon O, Kearney M, Blackson J, et al. Prolonged HIV-1 remission and viral rebound in an individual treated during Hyperacute infection. Paris, France: International AIDS Society; 2017. Available: http://programme.ias2017.org/Abstract/Abstract/3034 Google Scholar
- Henrich TJ, Hanhauser E, Marty FM, Sirignano MN, Keating S, Lee T-H, et al. Antiretroviral-free HIV-1 remission and viral rebound after allogeneic stem cell transplantation: report of 2 cases. Ann Intern Med. 2014;161:319–27. https://doi.org/10.7326/M14-1027.View ArticlePubMedPubMed CentralGoogle Scholar
- Hill AL, Rosenbloom DIS, Goldstein E, Hanhauser E, Kuritzkes DR, Siliciano RF, et al. Real-time predictions of reservoir size and rebound time during antiretroviral therapy interruption trials for HIV. PLoS Pathog. 2016;12:e1005535. https://doi.org/10.1371/journal.ppat.1005535.View ArticlePubMedPubMed CentralGoogle Scholar
- Finzi D. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science (80- ). 1997;278:1295–300. https://doi.org/10.1126/science.278.5341.1295.View ArticleGoogle Scholar
- Ho Y-C, Shan L, Hosmane NN, Wang J, Laskey SB, Rosenbloom DIS, et al. Replication-competent noninduced proviruses in the latent reservoir increase barrier to HIV-1 cure. Cell. Elsevier Inc. 2013;155:540–51. https://doi.org/10.1016/j.cell.2013.09.020.Google Scholar
- Luzuriaga K, Gay H, Siemniak C, Sanborn KB, Somasundaran M, Rainwater-Lovett K, et al. Viremic relapse after HIV-1 remission in a perinatally infected child. N Engl J Med. 2015;372:784–6. https://doi.org/10.1056/NEJMc1414210.View ArticleGoogle Scholar
- Rose R, Nolan DJ, Maidji E, Stoddart CA, Singer EJ, Lamers SL, et al. Eradication of HIV from tissue reservoirs: challenges for the cure. AIDS Res Hum Retrovir. 2017; [Epub ahead of print]; https://doi.org/10.1089/aid.2017.0072.
- Churchill MJ, Deeks SG, Margolis DM, Siliciano RF. HIV reservoirs: what, where, and how to target them. Nat Rev Nature Publishing Group. 2016;14:55–60. https://doi.org/10.1038/nrmicro.2015.5.Google Scholar
- Benkirane M. Keynote address: HIV/AIDS and cancer cure: is it the same battle? International AIDS society. Paris: France; 2017. Available: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5632552/.Google Scholar
- Dimitrov DT, Kiem H-P, Jerome KR, Johnston C, Curative Regimen SJTA. Would decrease HIV prevalence but not HIV incidence unless targeted to an ART-Naïve population. Sci rep Nat Publ Group. 2016;6:22183. https://doi.org/10.1038/srep22183.Google Scholar
- Ananworanich J, Dubé K, Chomont N. How does the timing of antiretroviral therapy initiation in acute infection affect HIV reservoirs? Curr Opin HIV AIDS. 2015;10:18–28. https://doi.org/10.1097/COH.0000000000000122.View ArticlePubMedPubMed CentralGoogle Scholar
- Ananworanich J, Schuetz A, Vandergeeten C, Sereti I, de Souza M, Rerknimitr R, et al. Impact of multi-targeted antiretroviral treatment on gut T cell depletion and HIV reservoir seeding during acute HIV infection. PLoS One. 2012;7:e33948. https://doi.org/10.1371/journal.pone.0033948.View ArticlePubMedPubMed CentralGoogle Scholar
- Archin N, Liberty A, Kashuba A, Choudhary S, Kuruc J, Crooks A, et al. Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy. Nature. 2012;487:482–5. https://doi.org/10.1038/nature11286.View ArticlePubMedPubMed CentralGoogle Scholar
- Archin NM, Bateson R, Tripathy MK, Crooks AM, Yang K-H, Dahl NP, et al. HIV-1 expression within resting CD4+ T cells after multiple doses of Vorinostat. J Infect Dis. 2014:1–8. https://doi.org/10.1093/infdis/jiu155.
- Carcelain G, Autran B. Immune interventions in HIV infection. Immunol Rev. 2013;254:355–71. https://doi.org/10.1111/imr.12083.View ArticlePubMedGoogle Scholar
- Euler Z, Alter G. Exploring the potential of monoclonal antibody therapeutics for HIV-1 eradication. AIDS Res Hum Retrovir. 2015;31:13–24.View ArticlePubMedPubMed CentralGoogle Scholar
- Stephenson KE, Barouch DH. Broadly neutralizing antibodies for HIV eradication. Curr HIV AIDS Rep. 2016;13:31–7. https://doi.org/10.1007/s11904-016-0299-7.View ArticleGoogle Scholar
- Tebas P, Stein D, Tang WW, Frank I, Wang SQ, Lee G, et al. Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N Engl J Med. 2014;370(10):901. https://doi.org/10.1056/NEJMoa1300662.View ArticlePubMedPubMed CentralGoogle Scholar
- Stepan N. Eradication: ridding the world of diseases forever? Ithaca: Cornell University Press; 2011.Google Scholar
- Henderson RH. Eradication: lessons from the past. Bull World Health Organ. 1998;76:14–6.Google Scholar
- Fauci A. An HIV vaccine is essential for ending the HIV/AIDS pandemic. J Am Med Assoc. 2017;Google Scholar
- Roberts ET, Matthews DD. HIV and chemoprophylaxis, the importance of considering social structures alongside biomedical and behavioral intervention. Soc Sci Med Elsevier Ltd. 2012;75:1555–61. https://doi.org/10.1016/j.socscimed.2012.02.016.View ArticleGoogle Scholar
- Frieden TR, Foti KE, Mermin J. Applying public health principles to the HIV epidemic - how are we doing? N Engl J Med. 2015;373:2281–7.View ArticlePubMedGoogle Scholar
- Piot P, Bartos M, Larson H, Zewdie D, Mane P. Coming to terms with complexity: a call to action for HIV. Lancet. 2008;372:845–59. https://doi.org/10.1016/S0140-6736(08)60888-0.View ArticlePubMedGoogle Scholar
- Kripke K, Njeuhmeli E, Samuelson J, Schnure M, Dalal S, Farley T, et al. Assessing progress, impact, and next steps in rolling out voluntary medical male circumcision for HIV prevention in 14 priority countries in eastern and southern Africa through 2014. PLoS One. 2016;11:e0158767. https://doi.org/10.1371/journal.pone.0158767.View ArticlePubMedPubMed CentralGoogle Scholar
- Granich R, Gupta S, Hall I, Aberle-Grasse J, Hader S, Mermin J. Status and methodology of publicly available national HIV Care continua and 90-90-90 targets: a systematic review. PLoS Med. 2017;14:e1002253. https://doi.org/10.1371/journal.pmed.1002253.View ArticlePubMedPubMed CentralGoogle Scholar
- Coltart CEM, Lindsey B, Ghinai I, Johnson AM, Heymann DL. The Ebola outbreak, 2013 – 2016: old lessons for new epidemics. Phil Trans R Soc B. 2017;372:2013–6.Google Scholar