The source and fate of Mycobacterium tuberculosis complex in wastewater and possible routes of transmission

BMC Public Health

Table 2 Detection of MTBC organisms using sequencing approaches

Specific MTBC organism	Sample matrix	Study location	Detection method	Sequencing method	Reference
Mycobacterium avium, Mycobacterium abscessus, Mycobacterium bovis, Mycobacterium kansasii, Mycobacterium marinum	Wastewater	Hong Kong	Illumina HTS	HTS-based metagenomic analysis	[122]
Mycobacterium sp., Mycobacterium fortuitum	Wastewater and sludge	China	Illumina HiSeq	Metagenomic sequencing	[123]
Mycobacterium sp		China	Illumina Hiseq	Paired-end sequencing	[114]
Mycobacterium sp	Wastewater	South Africa	16S-rRNA-Based Amplicon Sequencing	Paired-end sequencing	[115]
Mycobacterium sp	wastewater	Singapore	Illumina HiSeq2500	Metagenomic sequencing	[117]
Mycobacterium sp	wastewater	Vietnam	Illumina TruSeq	Cluster generation and paired-end sequencing	[116]
Mycobacterium sp	Biosolids	Colombia	Illumina MiSeq	Metagenomics and 16S-amplicons sequencing	[118]
Mycobacterium tuberculosis, Mycobacterium sp	wastewater	USA	Illumina MiSeq	Shotgun metagenomic analyses	[121]
Mycobacterium sp	wastewater	Taiwan	Illumina HiSeq PE150	Paired-end sequencing	[124]
Mycobacterium sp	wastewater	USA	NGS—next-generation sequencing	Shotgun whole genome sequencing	[119]

ISSN: 1471-2458