Fig. 3

PCR amplification of plasmodial DNA isolated from the non-imported case from Pesaro, targeting sequences discriminating between Plasmodium ovale curtisi and P. ovale wallikeri. Two pairs of primers were used: first one pair (rOVA1WC-rOVA2WC) is used to amplify both subspecies, and second one pair (rOVA1v-rOVA2v) is specific for P. ovale wallikeri diagnosis (Fuehrer et al., 2012). (M) DNA marker; a Amplification results using rOVA1WC+ rOVA2WC primers; (1–2) patient sample replicates; (3) P. ovale curtisi control DNA; (4) P. ovale wallikeri control DNA; (5) negative control (no DNA); b Amplification results using rOVA1v + rOVA2v primers; (6-7) patient sample replicates; (8) P. ovale curtisi control DNA; (9) P. ovale wallikeri control DNA; (10) negative control (no DNA)