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Table 2 Testing of primers’ sequences for positive and negative controls DNA samples for leishmaniasis species

From: Prevalence of Leishmania species among patients with cutaneous leishmaniasis in Qassim province of Saudi Arabia

Positive/negative control DNA samples Source L. major assays L. infantum /donovani assays L. mexicana assays L. viannia assays L. tropica assays β-actin assays
L. major DNA Culture Positive Negative Negative Negative Negative Positive
L. tropica DNA Culture Negative Negative Negative Negative Positive Positive
L. infantum DNA Culture Negative Positive Negative Negative Negative Positive
L. donovani DNA Culture Negative Positive Negative Negative Negative Positive
L. mexicana DNA Culture Negative Negative Positive Negative Negative Positive
L. (V). braziliens DNA Culture Negative Negative Negative Positive Negative Positive
Vitiligo DNA Skin Negative Negative Negative Negative Negative Positive
Psoriasis DNA Skin Negative Negative Negative Negative Negative Positive
Basal cell carcinoma DNA Skin Negative Negative Negative Negative Negative Positive
Vitiligo blood DNA Blood Negative Negative Negative Negative Negative Positive
Psoriasis blood DNA Blood Negative Negative Negative Negative Negative Positive
Acne DNA Blood Negative Negative Negative Negative Negative Positive
Atopic dermatitis DNA Blood Negative Negative Negative Negative Negative Positive
SLE DNA Blood Negative Negative Negative Negative Negative Positive
Normal DNA Blood Negative Negative Negative Negative Negative Positive