Genetic variables | Analytical Techniques |
---|---|
Adiponectin gene: A/C SNP -4034 (rs822395), T/G SNP +45 in exon 2 (rs2241766) G/T SNP +276 in intron 2 (rs1501299) [56] Interleukin 6 gene: 174 G/C SNP(rs1800795) [57] Fat mass associated gene (FTO): rs9939609 and rs7204609 [58] | ABI prism 7000 sequence detector followed by allelic discrimination |
Melanocortin 4 receptor gene (MC4R) [59] | Sequencing of the entire coding region of the MC4R gene was performed on an automated DNA sequencer. |