Table 2 Blood biochemical and metabolic profiles.
Biochemical variables | Analytical Techniques |
|---|---|
Triglycerides, Total cholesterol, HDL-Cholesterol, HDL2/HDL3 fraction, LDL-cholesterol | Colorimetric assay |
Apolipoprotein B, A1, Lipoprotein(a), ASP (acylation-stimulating protein) | Nephelometry, enzyme immuno assay (EIA) and radioimmuno assasy (RIA) |
Non Esterified Free Acids (NEFA) | Chromatography |
Metabolical variables | Analytical Techniques |
Insulin, Glucose, Prealbumin, Uric acid, Homocysteine | Colorimetric assays, precipitation methods and nephelometry, enzyme immuno assay (EIA) and radioimmuno assasy (RIA) |
Oxidative state | Analytical Techniques |
Oxidised-LDL | Fluorimetry |
Malonyl-dialdehyde | HPLC |
Plasma total oxidative capacity | Spin electron resonance spectroscopy |
Vitamins | Analytical Techniques |
Tocopherol, retinol, vitamin C and folic acid | Chromatography |
Hormones | Analytical Techniques |
Thyrotropin (TSH), Grown hormone (GH), Follicle-stimulating hormone (FSH), Luteinizing hormone (LH), Adrenocorticotropin hormone (ACTH), Prolactin (PRL) | Human Pituitary Kit |
Ghrelin, Insulin, Leptin, PYY | Gut Hormone Kit |
Free tyroxine (FT4) | Radioimmuno assasy (RIA) |