Figure 1

Neighbor-joining tree of a 348 nt fragment (including gaps) of the HBV S gene from isolates of the present studied population and Genbank sequences representing genotypes A to H. Tree was constructed with the distance matrix calculated with the Kimura 2-parameter method and γ distribution using MEGA version 2.1. Bootstrap test of phylogeny was performed with 100 replications and values equal or greater than 69 are indicated on the branches. Sequences from the present study are named as followed R_P_NNN_G where: R = region of haemodialysis unit (A to V); P (H = haemodialysis groups and C = controls); NNN = number of the sequence; and G = genotype (A, D or F). Genotype representative sequences from the Genbank (loci and accession numbers) are: A (HHVBA – X75666, HPBSAG – M32138); B ( HPBADW2 – D00330, HPBADW3 – BD00331, S74815 – S74815); C (HHVBC – S75184, HHVCCHA – X75665; S81946 – S81946); D (RXXHEPA – V01460 , HPVPS12SA – X77309); E (HHVBASS – X75657 ); F (HBVADW4A – X69798; HHVBF – X75663); G (IG9227 – AF160501) and H (U91827 – U91827). The Woolly Monkey Hepatitis B Virus (WM046996) was utilized as outgroup.