Serum ferritin
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Diagnostic Medlab, Auckland
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Immunoturbidimetric test (Roche Diagnostics, Indianapolis) (Cat. No. 11661400)
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Serum C-Reactive Protein
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Diagnostic Medlab, Auckland
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Particle enhanced immunoturbidimetric assay (Roche Diagnostics, Indianapolis) (Cat. No. 03002039)
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Haemoglobin
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Diagnostic Medlab, Auckland
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SLS-Hb (sodium lauryl sulphate-Hb) method using automated haematology analyser XE-2100 (Sysmex Corporation, Auckland, NZ)
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Soluble transferrin receptor
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LabPlus, Auckland
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Latex bound anti-sTfR antibodies react with antigen in sample to form antigen/antibody complex. Following agglutination measured turbidimetrically at 570 nm
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Ascorbic acid
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LabPlus, Auckland
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Fe3+ ions added to ascorbate which is oxidised to dehydroascorbate. Fe2+ ions produced are measured by complexing them with ferrozine, the absorbance of the coloured complex is read at 560 nm. FeCl3 is added and incubation continued for 5 minutes. Concentration of ascorbate in specimen after suitable blank correction is proportional to change in absorbance over 5 minute period.
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Carotenoids (Retinol, Alpha- & Gamma-Tocopherol; Lutein/Zeaxanthin, Lycopene, Alpha- & Beta-Carotene, Beta-Cryptoxanthin
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Department of Medicine, University of Melbourne
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HPLC method C18 column with absorbance detection at 292 nm for the tocopherols, 325 nm for retinol and 450 nm for carotenoids.
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