|
Serum ferritin
|
Diagnostic Medlab, Auckland
|
Immunoturbidimetric test (Roche Diagnostics, Indianapolis) (Cat. No. 11661400)
|
|
Serum C-Reactive Protein
|
Diagnostic Medlab, Auckland
|
Particle enhanced immunoturbidimetric assay (Roche Diagnostics, Indianapolis) (Cat. No. 03002039)
|
|
Haemoglobin
|
Diagnostic Medlab, Auckland
|
SLS-Hb (sodium lauryl sulphate-Hb) method using automated haematology analyser XE-2100 (Sysmex Corporation, Auckland, NZ)
|
|
Soluble transferrin receptor
|
LabPlus, Auckland
|
Latex bound anti-sTfR antibodies react with antigen in sample to form antigen/antibody complex. Following agglutination measured turbidimetrically at 570 nm
|
|
Ascorbic acid
|
LabPlus, Auckland
|
Fe3+ ions added to ascorbate which is oxidised to dehydroascorbate. Fe2+ ions produced are measured by complexing them with ferrozine, the absorbance of the coloured complex is read at 560 nm. FeCl3 is added and incubation continued for 5 minutes. Concentration of ascorbate in specimen after suitable blank correction is proportional to change in absorbance over 5 minute period.
|
|
Carotenoids (Retinol, Alpha- & Gamma-Tocopherol; Lutein/Zeaxanthin, Lycopene, Alpha- & Beta-Carotene, Beta-Cryptoxanthin
|
Department of Medicine, University of Melbourne
|
HPLC method C18 column with absorbance detection at 292 nm for the tocopherols, 325 nm for retinol and 450 nm for carotenoids.
|
