Table 2 Biochemical analysis
Measure | Place of analysis | Method |
|---|---|---|
Blood analysis | ||
Serum ferritin | Diagnostic Medlab, Auckland | Immunoturbidimetric test (Roche Diagnostics, Indianapolis) (Cat. No. 11661400) |
Serum C-Reactive Protein | Diagnostic Medlab, Auckland | Particle enhanced immunoturbidimetric assay (Roche Diagnostics, Indianapolis) (Cat. No. 03002039) |
Haemoglobin | Diagnostic Medlab, Auckland | SLS-Hb (sodium lauryl sulphate-Hb) method using automated haematology analyser XE-2100 (Sysmex Corporation, Auckland, NZ) |
Soluble transferrin receptor | LabPlus, Auckland | Latex bound anti-sTfR antibodies react with antigen in sample to form antigen/antibody complex. Following agglutination measured turbidimetrically at 570 nm |
Ascorbic acid | LabPlus, Auckland | Fe3+ ions added to ascorbate which is oxidised to dehydroascorbate. Fe2+ ions produced are measured by complexing them with ferrozine, the absorbance of the coloured complex is read at 560 nm. FeCl3 is added and incubation continued for 5 minutes. Concentration of ascorbate in specimen after suitable blank correction is proportional to change in absorbance over 5 minute period. |
Carotenoids (Retinol, Alpha- & Gamma-Tocopherol; Lutein/Zeaxanthin, Lycopene, Alpha- & Beta-Carotene, Beta-Cryptoxanthin | Department of Medicine, University of Melbourne | HPLC method C18 column with absorbance detection at 292 nm for the tocopherols, 325 nm for retinol and 450 nm for carotenoids. |