Evaluation of the effectiveness of the human-baited double net traps and BG traps compared with the human landing catches for collecting outdoor Aedes albopictus in China

Dengue fever is one of the biggest threats to public health in China, causing huge disease burden and economic loss. Aedes-mosquito surveillance could be a cornerstone for predicting the risk of Aedes-borne diseases and evaluating the effect of vector management during diseases outbreaks. The human landing catch (HLC) method is regarded as the “gold standard” for catching Aedes mosquitoes, but it potentially exposes field professionals to vectors of known or unknown pathogens. Human-baited double net (HDN) was recommended to replace HLC for emergency monitoring in China when Aedes-borne diseases break out, but it had been reported with low efficiency for capturing Aedes mosquitoes. In this study, we compared HLC with HDN and BG traps for field Aedes albopictus monitoring, with the aim of evaluating the effectiveness of HDN replacing HLC and finding an effective and safe alternative to the HLC for monitoring Aedes albopictus. Six sites in Hangzhou, Shaoxing, and Yiwu, Zhejiang Province, China, were chosen to conduct outdoor HLC, HDN, and BG trap catches from June to October 2021. The tests were performed 3 h apart: 8:30–9:30 AM, 16:30–17:30 PM, and 17:30–18:30 PM. A total of 2330 adult mosquitoes were collected, and Aedes albopictus was the most abundant species in all three catches with 848(98.95%), 559(97.39%) and 867 (96.44%) caught in HLC, HDN and BG traps respectively. Compared to HLC, HDN collected significantly less Ae. albopictus and Ae. albopictus females per trapping period (P < 0.001, P < 0.001), whereas no statistical differences were found between the HLC and BG trap (P = 0.970, P > 0.05). Statistically significant positive spatial correlations for Ae. albopictus sampling was found between HLC and HDN traps (r = 0.543, P < 0.001) and HLC and BG traps (r = 0.658, P < 0.001). In conclusion, both the BG trap and HDN have a significant positive spatial correlation with HLC, making them safer alternatives to HLC for Ae. albopictus monitoring in China. However, with better a sampling efficiency, being less labor intensive, and no human-baited attraction bias, the BG trap could be a better choice than the HDN trap.

Evaluation of the effectiveness of the human-baited double net traps and BG traps compared with the human landing catches for collecting outdoor Aedes albopictus in China Yuyan Wu 1 † , Juan Hou 1 † , Qinmei Liu 1 , Jinna Wang 1 , Tianqi Li 1 , Mingyu Luo 1 and Zhenyu Gong 1*

Background
Dengue fever is a high-risk vector-related infectious disease with rapid global transmission [1].It is believed that the number of dengue cases worldwide has increased 30 times in the past 50 years, with 40% of the global population at risk.It is mainly distributed in tropical and subtropical Africa, the Americas, Southeast Asia, the Western Pacific and Europe [2][3][4].Since 2010, most outbreaks have been concentrated in the western Pacific regions, such as Singapore, China, and Malaysia [5].With the distribution range of Aedes albopictus in China continuing to expand, ≥ 168 million people are at high risk of dengue fever annually, which makes dengue fever a major public health threat in China [6,7].Without vaccines and effective medicine, monitoring and controlling arthropod vectors are important means of controlling dengue fever [7].Mosquito surveillance has been regarded as the cornerstone for the development of mosquito control operations because surveillance information can guide control efforts and evaluate the efficacy of vector management [8].Ae. albopictus is the only vector for transmitting dengue in Zhejiang province, as well as the main vectors responsible for dengue transmission in mainland China, [9,10].Monitoring Ae. albopictus is important for predicting the dengue outbreak risk and evaluating the effects of dengue epidemic control in Zhejiang, China.However, for Ae.albopictus, none of the existing traps without human attractants is as effective as the traditional human landing catch method (HLC) [11,12].
HLC uses humans as attractants, and mosquitoes are collected when they land on exposed legs.At present, HLC is still considered as the "gold standard" because of its high efficacy in monitoring Aedes mosquitoes [9,13].However, when Aedes-borne diseases break, they pose a risk to field work professionals, because human attractants should continue exposing themselves to infective bites of known and unknown pathogens-bearing vectors.Thus, many new methods have been developed, such as the human-baited double net trap (HDN) and the BG trap [9].The HDN consists of two box nets and uses human bait as a mosquito attractant.A large net contains a smaller net, and the outer net is raised off the ground to attract and collect mosquitoes between the two nets.People sit or stand in the inner net to attract mosquitoes, while others collect the mosquitoes between the two nets [9,14].HDN is much safer than HLC because the human-baiter is protected from mosquito landing and biting, and the outer collector can be protected by long-sleeved clothing and repellents [9].Thus, HDN was recommended by China center for diseases control and prevention (CDC) for emergency monitoring when Aedes-borne diseases break out.However, HDN has been reported with low efficiency for capturing Aedes mosquitoes and few studies has evaluated its effectiveness of replacing HLC in China [12,15].The BG trap uses carbon dioxide (CO 2 ) and an attractant that mimics human scent to attract mosquitoes, and once mosquitoes are attracted and fly to the trap, they are automatically caught [16].In America, BG-Sentinel traps have been considered the "gold standard" for collecting Aedes stegomyia mosquitoes [17].Although HLC has traditionally been considered the most effective method for monitoring highly anthropophilic mosquitoes, few evaluation studies have carried out to compared HLC with HDN and some other newly developed methods for Ae.albopictus collection in China.
In this study, we attempted to verify the effectiveness of HDN and BG traps on Ae. albopictus surveillance and their efficiency in sampling adult Ae. albopictus compared to that of HLC.We attempted to determine the relationships between these three methods for monitoring Ae. albopictus and to evaluate the effectiveness of HDN replacing HLC, at the same time to explore the potential of BG traps to replace HLC for Aedes monitoring.

Study sites
This study was conducted on sunny and cloudy days from June to September 2021 in three cities where had ever experienced outbreaks of dengue fever in history.Hangzhou, Shaoxing, and Yiwu located in the north and center of Zhejiang Province, China, all of which have a subtropical monsoon climate, with a temperature ranged 20 ~ 34 ℃, rainfall ranged 91 ~ 228 mm from June to September 2021.Six field-monitoring sites (site one to site six) representing urban, suburban, and downtown environments were selected for mosquito sampling.The study was conducted in areas without reported local mosquito-borne diseases such as dengue fever, chikungunya fever, and Zika cases, before or during the study period in 2021.The details of the locations are shown in Table 1; Fig. 1.

Study participants
Informed consent was obtained and 24 volunteers aged 23-54 years (13males and 11females) were recruited for participation.Training was carried out for all the participants before the study.

Study design
The catches were performed three times a day in peak period of Ae. albopictus activity in Zhejiang province, China.Each session lasted for one hour.These were 8:30-9:30 AM, 16:30-17:30 PM, and 17:30-18:30 PM, representing morning, afternoon and evening.At each site, three catches were performed simultaneously, 10 m apart (Fig. 2) [18].The 24 volunteers were randomly separated into six groups, and each group containing four participants (from A to D) was responsible for three catches at one site.Participants A and B were designated human baits to lure mosquitoes in the HLC or HDN catches.Participant C was responsible for collecting mosquitoes in the HDN catch, cooperating with participants A or B. Participant D was responsible for capturing mosquitoes with the BG trap.To minimize the attractant bias, human baits A and B of HLC and HDN were exchanged between the first and second 30 min of each time, while participants C and D always acted as collectors in HDN catches and operators in BG-trap catches, respectively (Fig. 3).In each site, the capture was carried out in two sunny or cloudy days with similar climate factors that are at least 14 days apart according to the weather forecast.To minimize the possible time bias between the first and second 30 min, participants A and B changed orders in HLC and HDN catches the next day.The per-trapping period lasted for 30 min.The details are shown in Fig. 2.

Mosquito sampling Human landing caches
The participant who performed the human landing catch exposed his right leg and collected mosquitoes landing on his leg (his left leg was protected by long pants), using a portable battery-powered aspirator.

Human-baited double net trap (HDN)
The patented double net trap developed by the Chinese Center for Disease Control and Prevention was used, with an outer net measuring 180 cm long, 180 cm wide, and 150 cm high and an inner net measuring 120 cm long, 120 cm wide, and 200 cm high.The bottom of the outer net was raised 35 cm above the ground, whereas the bottom of the inner net was hanging to the ground.One human baiter sat inside the inner set with two legs exposed, and one collector with long-sleeved clothing collected attracted mosquitoes between the two nets.No repellent was used by the human baiter or collector during the study period.

BG trap
BG traps (version: BG-Mosquitaire CO2) developed by the BioGents GmbH Company (Regensburg, Germany,  Collected mosquitoes were taken to the Zhejiang Provincial Center for Disease Control and Prevention Laboratory, killed by freezing, and identified using taxonomic keys [13].

Statistical analysis
Statistical analyses were performed using Statistical Package for the SPSS (version 23.0) [19].Generalized linear mixed models (GLMMs) were used to analyze the effect of different catches on the total number of Ae. albopictus (both male and female), and Ae.albopictus females caught per monitoring period (30 min), based on negative binomial regression.The dependent variables were modeled via GLMMs controlling for independent random variables ("days, " in this case) to test the statistical significance of fixed independent variables ("catches, " "sites, " "time" and "baits").The means and standard errors associated with GLMMs were calculated.Pearson correlation analysis was used for spatial sampling yields between HDNs and HLCs and between BG traps and HLCs.Statistical significance was set at P < 0.05.
Significant differences between total number of Ae. albopictus adults and Ae.albopictus females alone were detected in the three catches (GLMM, Ae. albopictus, F (1,122) = 14.293,P < 0.05; Ae. albopictus females, F (1,122) = 28.759,P < 0.05).Compared to HLC, HDN collected significantly less Ae.albopictus and Ae.albopictus females per trapping period, whereas no statistical differences were observed between the HLC and BG traps (Table 3).The sampling efficiency of the HDN and BG traps for Ae.albopictus were approximately 0.66 and 1.02 times that of HLC, respectively, and for Ae.albopictus females, the sampling efficiencies were 0.54 and 0.88 times that of HLC, respectively (Table 2).Though the mean Ae. albopictus catch by HDN was significantly lower than that by HLC, a significantly positive spatial correlation between HLC and HDN for Ae.albopictus and Ae.albopictus female was found (Ae.albopictus: r = 0.543, P < 0.001; Ae. albopictus females; r = 0.694, P < 0.001).A positive spatial correlation between the HLC and HDN was also detected (Ae. albopictus: r (51) = 0.658, P < 0.001; Ae. albopictus females; r = 0.669, P < 0.001).

Discussion
HLC, HDN, and BG traps are widely used to monitor adult mosquitoes worldwide.The results of the present study indicate that the individuals of Ae. albopictus caught by both the HDN and BG traps were positively correlated with that caught by HLC.The Ae. albopictus sampling efficiency of HLC was significantly higher than that of HDN, which was statistically similar to that of the BG trap.This result is highly consistent with the comparisons conducted between HLC and HDN by Gao et al. in Shanghai [14].
Zhejiang is located in southeast China and Ae.albopictus was the only vector responsible for Aedes-borne diseases such as dengue fever and chikungunya fever.Thus, the subjects in this study were Ae.albopictus, particularly Ae. albopictus females because only female mosquitoes would take blood and transmit diseases, the role of which is crucial in monitoring [20].Based on the results of this study, the number of Ae. albopictus and Ae.albopictus females collected by HDN were significantly lower than those collected by HLC (both P < 0.001).Compared to HLC, only 0.66 times of Ae. albopictus and 0.54 times of Ae. albopictus females were collected by HDN, which was similar to the results from Shanghai, Uganda, and Nigeria studies, with individuals of Ae. albopictus captured by HDN being 0.4, 0.5 and 0.25 times that of HLC, respectively [14,21,22].This might be attributed to the trap design of the two box nets in the HDN, which limited excessive attractive emanations from the hosts inside the inner net.This suggests that HDN may underestimate Ae. albopictus during the monitoring period.Too moderate a sampling efficiency would also cause HDN to fail in distinguishing the effect differences of Aedes mosquito management and fail in guiding control efforts when Aedes-borne diseases break [15].Owing to the relatively small sample size, no statistically significant variation in human baits was found in this study.However, human-baited bias existed in traps that used humans as attractors, as previously reported [14,23].The reason might be that heat, water vapor, CO 2 , and various odors that lure mosquitoes emanating from different individuals differed [23].HDN was supposed to  reduce human-baited bias because of the design of the two box nets limiting attractive emanations, which is also the reason for its low sampling efficiency.The dilemma of HDN is that the design of double nets has lost its efficiency in attracting mosquitoes and requires more labor to make it safer and less bias to human bait.The BG trap has been used for Aedes-mosquitoes monitoring in North America, Singapore, and Australia [24][25][26].As there were no human baits used, the results would be more comparable among different locations.
According to previous research, the CO 2 flow in the BG traps was set to 0.3 L/min in this study, which was considered to be the most appropriate for Ae.albopictus monitoring [19].Sampling efficiency of BG traps for Ae.albopictus and Ae.albopictus females were statistically similar to those of the HLC.Similar results were reported by Krockel et al. in Brazil [27].In addition, compared to HLC and HDN catches, the BG trap method could save more labor.One field professional could operate several BG traps to monitor the mosquito density in several places at the same time, while using HLC or HDN catches would require one or two laborers to be in one place for at least 30 min.
This study was conducted during the peak biting periods of Ae. albopictus, and more mosquitoes (mainly Ae. albopictus) tended to be caught at the first half hour (16:30 − 17:00), it might be caused by the bloodbucking habits of Ae. albopictus on one hand.On the other hand, it might be caused by continuous capture in the afternoon/evening, because mosquito density might be reduced due to repeated capture.Secondly, the distance between catches were set within 10 m by some previous researches when comparing their efficiency in capturing mosquitoes [9,12,28,29].An pre-experiment was also conducted in this study, and the results showed that the density of Ae. albopictus was more probably remaining consistent within a range of 10 m (unpublished data).Thus 10 m were set apart between each catch to balance between reducing physical interference among three catches and the consistency of mosquito density in the environment where three catches were located.But the interference among catches might not be removed thoroughly.Therefore, caution should be exercised when extrapolating all these results.Besides, the research object was only Ae. albopictus.In the future, studies should be conducted to explore the relationship between different monitoring methods on other mosquitoes, such as Anopheles and Culex species in China.

Conclusions
With a significantly positive spatial correlation with HLC, both the BG trap and HDN could be safer alternatives to HLC for Aedes albopitus monitoring in China.Because BG traps have better sampling efficiency, are less labor-intensive, and do not have a human bait attraction bias, they might be a better choice than HDN traps.

Fig. 1
Fig. 1 Locations of the six sites for mosquito monitoring comparison among three catches in Zhejiang province, China

Fig. 3 Fig. 2
Fig. 3 Comparison among three catches at each site

Fig. 4
Fig. 4 Difference in the number of mosquitoes captured at different times of the day

Table 1
Geographical Information for the six Mosquito Sampling Sites

Table 2
Mosquito species and sex composition captured using HLC, HDN and BG traps

Table 3
Differences of the number of individuals captured per trapping period among three

catches Catches Ae. albopictus Ae. albopictus Females Estimate SE
Mean +/− SE differences in the least squares means associated with the mixed linear models for the number of individuals per trapping period among three catches.