Component | Key constructs | Measures | |
---|---|---|---|
Primary / secondary outcomes | Other measures | ||
Fasting blood draws | Glycaemic control | Glycosylated haemoglobin (HbA1c %, HbA1c mmol/mol)* | |
Fasting glucose metabolism | Glucose and Insulin | ||
Fasting lipid metabolism | LDL, HDL, Total cholesterol, Triglycerides | ||
Inflammatory markers | Hs-CRP, TNF-α, IL-6, IL-1B, Adiponectin, Leptin | IFNy, IL-10, IL-12, IL-8, IL-4 | |
Neurotrophic factors | BDNF, IGF-1 | NGF, VEGF-A | |
Genetic risk factors | ApoE4 | ||
2 h Oral Glucose Tolerance Test | Postprandial metabolism of glucose & insulin | iAUC glucose, iAUC insulin, 2 h post-prandial glucose, 2 h post-prandial insulin | |
Anthropometric | Body weight & body composition | Weight, waist circumference, DXA assessed total and regional fat mass, lean mass, total body fat percentage, DXA software estimated visceral adipose tissue mass | Height, BMI |
Vascular | Blood pressure & heart rate | Resting systolic blood pressure, diastolic blood pressure, mean arterial pressure | Resting heart rate |
Flow Mediated Dilation | Post-deflation peak vessel diameter compared to resting diameter (FMD%) | Shear rate (cm/s), Shear rate AUC | |
Cambridge Neuropsychological Test Automated Battery (CANTAB) | Visual learning and new memory | Paired Associates Learning total errors (adjusted)b (PAL TEA), PAL total errors 12 shapes (adjusted) | PAL total errors, PAL total errors 12 patterns |
Reaction Time | Reaction Time (RTI) simple median reaction time, RTI simple median movement time & RTI median 5-choice reaction time, RTI median 5-choice movement time | RTI simple error score (all), RTI total error score (5-choice) | |
Visual matching ability and visual recognition memory | Delayed Matching to Sample (DMS) percent correct, DMS median correct latency | DMS total errors, DMS total correct (12 s delay) | |
Working memory | Spatial Working Memory (SWM) between errors, SWM between errors (12 boxes), SWM strategy (6–12 boxes) | SWM total errors, SWM total errors (12 boxes) | |
Cognitive function | Global cognition | Addenbrooke Cognitive Examination-Revised (ACE-R) Mini Mental State Examination | |
Device-based monitoring (thigh-worn activPAL) | Sedentary behaviour | Overall sitting timea, Overall prolonged sitting time (≥30 min), Work sitting time, Work prolonged sitting time | |
Sedentary time accumulation | Usual bout duration, Alpha (overall and work) | ||
Active breaks | Active breaks (n per day overall and work) | ||
Physical activity | Standing, Stepping, LPA, MVPA (all overall and during work) | ||
Sleep | Total sleep time, Sleep efficiency | Sleep onset latency, Wakefulness after sleep onset | |
Device-based monitoring (wrist-worn Actigraph GT3X+) | Sleep | Total sleep time, Sleep efficiency | Sleep onset latency, Wakefulness after sleep onset |