BoE | Abbreviation | Associated Toxicant | Matrix |
 Carbon monoxide | CO | Carbon monoxide | Exhaled breath |
 Total nicotine equivalents (nicotine, cotinine, 3-hydroxycotinine and their glucuronide conjugates) | TNeq | Nicotine | Urine |
 Total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol | Total NNAL | 4 (methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) | Urine |
 Total N-nitrosonornicotine | Total NNN | NNN | Urine |
 3-hydroxypropylmercapturic acid | 3-HPMA | Acrolein | Urine |
 3-hydroxy-1-methylpropylmercapturic acid | HMPMA | Crotonaldehyde | Urine |
 S-phenylmercapturic acid | S-PMA | Benzene | Urine |
 Monohydroxybutenyl-mercapturic acid | MHBMA | 1,3-butadiene | Urine |
 2-cyanoethylmercapturic acid | CEMA | Acrylonitrile | Urine |
 4-aminobiphenyl | 4-ABP | 4-Aminobiphenyl | Urine |
 o-toluidine | o-Tol | o-Toluidine | Urine |
 2-aminonaphthalene | 2-AN | 2-Aminonaphthalene | Urine |
 1-hydroxypyrene | 1-OHP | Pyrene | Urine |
 2-hydroxyethylmercapturic acid | HEMA | Ethylene oxide | Urine |
 N-acetyl-S-(2-carbamoylethyl)cysteine and N-acetyl-S-(2-hydroxy-2-carbamoylethyl)cysteine | AAMA and GAMA | Acrylamide | Urine |
BoBE | Abbreviation | Biological Effect | Matrix |
 8-Epi-Prostaglandin F2α Type III | 8-Epi-PGF2α Type III | Oxidative stress | Urine |
 White blood cell count | WBC count | Inflammation | Whole blood |
Nicotine pharmacokinetic parameters | Abbreviation | ||
 Time to maximum observed plasma concentration for nicotine | tmax | ||
 Maximum observed plasma concentration for nicotine | Cmax | ||
 Area under the nicotine plasma concentration-time curve from time zero to the last measurable concentration | AUC0-last | ||
Safety parameters | Other measures | ||
 Medical history of study subjects | Product satisfaction questionnaire | ||
 Physical examination | Puff count during pharmacokinetic assessment period | ||
 Vital signs | Creatinine concentration (24-h urine) | ||
 Electrocardiogram (ECG) | Product use during exposure phase | ||
 Clinical laboratory evaluations | Nicotine molar metabolite ratio | ||
 Lung function test |  | ||
 Adverse event /serious adverse event recording |  |