Table 2 Study measurements
Study parameters | Method/Instrument |
|---|---|
Blood pressure in mmHg | Electronic BP monitor (OMRON HEM-7130-L). Instrument validated by International Protocol for device validation O’Brien et al, (Working Group on Blood Pressure Monitoring of the European Society of Hypertension) |
Height in Cm | Stadiometer (Seca 213) |
Weight in meter | Digital weighing scales (Seca HN286) |
Wait circumference in inches | Non elastic measuring tapes (Seca 201) |
Plasma glucose in mg/dl | Enzymatic Colorimetric Assay method (modified GOD-PAP method based on the work of Trinder, 1969) |
Glycosylate Hb (HbA1c), % | High Performance Liquid Chromatography (HPLC) |
Cholesterol in mg/dl | CHOD-PAP enzymatic. Enzymatic In vitro Calorimetric method (automated clinical chemistry analyzer Roche/Hitachi 902) |
Triglycerides in mg/dl | Enzymatic Calorimetric test, GPO-PAP method |
High density lipoprotein cholesterol (HDL) in mg/dl | Direct measurement. Automated method for direct determination. |
Low density lipoprotein cholesterol (LDL) | Direct measurement. Homogeneous Enzymatic Assay for direct quantitative determination (automated clinical chemistry analyzer Roche/Hitachi 902), for samples with triglycerides more than 400Â mg/dl. |
Very low density lipoprotein cholesterol (VLDL) | Estimation using Friedewald and Fredrickson Formula, 1972 |
Nicotine metabolites screening in urine. | Lateral flow chromatographic immunoassay for the detection of cotinine in human urine at the cut-off concentration of 200Â ng/ml. |