Skip to main content

Table 2 Study measurements

From: A PROgramme of Lifestyle Intervention in Families for Cardiovascular risk reduction (PROLIFIC Study): design and rationale of a family based randomized controlled trial in individuals with family history of premature coronary heart disease

Study parameters Method/Instrument
Blood pressure in mmHg Electronic BP monitor (OMRON HEM-7130-L). Instrument validated by International Protocol for device validation O’Brien et al, (Working Group on Blood Pressure Monitoring of the European Society of Hypertension)
Height in Cm Stadiometer (Seca 213)
Weight in meter Digital weighing scales (Seca HN286)
Wait circumference in inches Non elastic measuring tapes (Seca 201)
Plasma glucose in mg/dl Enzymatic Colorimetric Assay method (modified GOD-PAP method based on the work of Trinder, 1969)
Glycosylate Hb (HbA1c), % High Performance Liquid Chromatography (HPLC)
Cholesterol in mg/dl CHOD-PAP enzymatic. Enzymatic In vitro Calorimetric method (automated clinical chemistry analyzer Roche/Hitachi 902)
Triglycerides in mg/dl Enzymatic Calorimetric test, GPO-PAP method
High density lipoprotein cholesterol (HDL) in mg/dl Direct measurement. Automated method for direct determination.
Low density lipoprotein cholesterol (LDL) Direct measurement. Homogeneous Enzymatic Assay for direct quantitative determination (automated clinical chemistry analyzer Roche/Hitachi 902), for samples with triglycerides more than 400 mg/dl.
Very low density lipoprotein cholesterol (VLDL) Estimation using Friedewald and Fredrickson Formula, 1972
Nicotine metabolites screening in urine. Lateral flow chromatographic immunoassay for the detection of cotinine in human urine at the cut-off concentration of 200 ng/ml.
  1. mg/dl milligram/deci litre, Cm Centimetre, mmHg milli meters of mercury, nd ng/dl nano gram/deci litre