Table 4 Laboratory measurements
Clinical Parameter | Laboratory parameter | Method |
|---|---|---|
Diabetes | Plasma glucose | Enzymatic Colorimetric Assay method (modified GOD-PAP method based on the work of Trinder, 1969) |
Dyslipidemia | Cholesterol | Enzymatic In vitro Calorimetric method (automated clinical chemistry analyzer Roche/Hitachi 902) |
Triglycerides | Enzymatic Calorimetric test (Based on the work by Wahlefeld using lipoprotein lipase from microorganisms for rapid and complete hydrolysis of triglycerides to glycerol followed by oxidation to dihydroxyacetone phosphate and hydrogen peroxide | |
High density lipoprotein cholesterol (HDL) | Automated method for direct determination | |
Triglycerides | Estimation using Friedewald and Fredrickson Formula, 1972 | |
Low density lipoprotein cholesterol (LDL) | Homogeneous Enzymatic Assay for direct quantitative determination (automated clinical chemistry analyzer Roche/Hitachi 902), for samples with triglycerides more than 400 mg/dl | |
Very low density lipoprotein cholesterol (VLDL) | Estimation using Friedewald and Fredrickson Formula, 1972 | |
Blood Routine | Hemoglobin | Indirect Cyanmethemoglobin method |