Fig. 1
From: Comparison of IgG against plastic resin in workers with and without chemical dermatitis
Immuno-dot blotting for detecting IgG which reacts with chemical-HSA adducts in human serum. A mixture of HSA (100 μM) and chemicals in buffer at pH 7.4, 8.0, or 9.2 was blotted on an NC membrane in the order from a low mixture rate (top) to high mixture rate (bottom), BADGE, XDA, or BEE : HSA in molecular bases ranged from 1, 12.5:1, 50:1, 100:1, 200:1, and 1,000:1. Product-X ranged from 0.01, 0.1, 1.25, 0.5, 1, 2, 4 %(w/v) in 100 μM HSA solution