Table 1 Details of the primers for HCV amplification
Targeted fragment | Name | Sequence of oligonucleotide (5’-3’) | Position (on H77) |
|---|---|---|---|
Fragment C/E1 | C/E1-F1 | ACTGCCTGATAGGGTGCTTGC | 288-308 |
C/E1-R1 | GGTGACCAGTTCATCATCATATCC | 1301-1324 | |
C/E1-F2 | CCTTCCTGGTTGCTCTTTCTCTAT | 845-868 | |
C/E1-R2 | GTTCATCATCATATCCCAAGCCAT | 1293-1315 | |
Fragment NS5b | NS5b-F1 | CCAATTVVCACTACHATCATGGC | 7995-8017 |
NS5b-R1 | TGGRGTGTGTCKDGCTGTTTCC | 8789-8810 | |
NS5b-F2 | CGTATGATACCCGCTGTTTTGA | 8254-8275 | |
NS5b-R2 | CCTGGTCATAGCCTCCGTGAA | 8616-8636 |