Figure 2

Individual level data for the blood donor's (unfasted group) showing unmetabolised folic acid nM/L plotted against plasma total folate nM/l. Outliers are included in this figure. The box plots on the x and y axis respectively indicate the univariate distribution of plasma folate and plasma unmetabolised folic acid. In each box plot the line across the box indicates the median point, the whiskers indicate the range of the data, and the 25 and 75% centiles are indicated by the box itself. the first. At this lower voltage specifically the folic acid peak decreases by about 95%, thus permitting not only its definitive identification, but also facilitation of placement of baselines for quantification of peak heights using an Empower (Waters) data system. The limit of detection (S/N = 3) was 0.15 nM in plasma, and the intra assay CV = 3%.