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Table 1 Overview of measurements per visit

From: Early diagnosis of asthma in young children by using non-invasive biomarkers of airway inflammation and early lung function measurements: study protocol of a case-control study

Media/method   ED I ED II ED III Follow- up Definite diagnosis
Exhaled breath Nitric Oxide
  Volatile organic compounds
Exhaled breath condensate Cytokines (IL1a,-2,-4,-5,-6,-10,- 12p70,-13,-18, IFNg, TNFa)
  Chemokines (MIP1a, MIF, eotaxin, RANTES, IL8, MCP1)
  Adhesion molecules (sICAM)
Blood White blood cell count, differentiation, number of eosinophils     
  Total Immunoglobulin E (IgE), and specific IgE    
  Regulatory T cells     
  Gene polymorphism (e.g. in IL-4, IL-13, TNF-alpha, ADAM33)     
  Gene expression (e.g. in IL-4, IL-13, TNF-alpha)     
  Anti bodies against Mycoplasma en Chlamydia pneumoniae     
Saliva Colonisation of Pneumococcen, Haemophilus (para)influenza, Staphylococcus aureus    
Faeces Colonisation of E.Coli en Clostridium difficile     
Lung function test Airway resistance (MicroRint) before and after bronchodilator  
  Dynamic spirometry (MEFV, FEV1, FVC, MEF50) before and after bronchodilator     
  Histamine provocation test     
Questionnaire Parental administrated respiratory symptoms (ISAAC)
  Demographic factors (e.g. smoking, pets)
  Parental administrated Quality of life (FSII)
  1. ED I/II/III = Consecutive measurements in early diagnosis phase