Table 1 Overview of measurements per visit

Exhaled breath

Nitric Oxide

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Volatile organic compounds

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Exhaled breath condensate

Cytokines (IL1a,-2,-4,-5,-6,-10,- 12p70,-13,-18, IFNg, TNFa)

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Chemokines (MIP1a, MIF, eotaxin, RANTES, IL8, MCP1)

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Adhesion molecules (sICAM)

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Nitrate/nitrite

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Blood

White blood cell count, differentiation, number of eosinophils

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Total Immunoglobulin E (IgE), and specific IgE

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Regulatory T cells

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Gene polymorphism (e.g. in IL-4, IL-13, TNF-alpha, ADAM33)

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Gene expression (e.g. in IL-4, IL-13, TNF-alpha)

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Anti bodies against Mycoplasma en Chlamydia pneumoniae

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Saliva

Colonisation of Pneumococcen, Haemophilus (para)influenza, Staphylococcus aureus

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Faeces

Colonisation of E.Coli en Clostridium difficile

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Lung function test

Airway resistance (MicroRint) before and after bronchodilator

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Dynamic spirometry (MEFV, FEV₁, FVC, MEF₅₀) before and after bronchodilator

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Histamine provocation test

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Questionnaire

Parental administrated respiratory symptoms (ISAAC)

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Demographic factors (e.g. smoking, pets)

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Parental administrated Quality of life (FSII)

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