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Blood analysis
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All analysed by LabPlus, Auckland City Hospital, Auckland
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Fasting plasma insulin
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Micro-particle enzyme immunoassay technology (MEIA), Abbott Diagnostics
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Fasting plasma glucose
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An enzymatic colourimetric assay; Roche Glucose reagent kit (Cat. No. 1876899)
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Total cholesterol
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Enzymatic colourimetric method (Roeschlau and Allain). Roche Cholesterol reagent kit (Cat. No. 1491458)
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Triglycerides
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Enzymatic conversion. Roche Triglycerides reagent kit (Cat. No. 1727711)
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HDL-C
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Homogenous enzymatic colourimetric assay. Roche HDL-Cholesterol Plus reagent kit (Cat. No. 04713214)
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LDL-C
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Calculated
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Serum calcium
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Colourimetric assay. Roche Calcium reagent kit (Cat. No. 1730240)
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Serum albumin
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Colourimetric assay. Roche Albumin Plus reagent kit (Cat. No. 1970909)
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Serum high sensitivity CRP
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Particle enhanced immunoturbidimetric assay. Roche CRPLX reagent kit (Cat. No. 03002039)
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Serum parathyroid hormone
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"ECLIA" electrochemiluminescence immunoassay. PTH Reagent Pack (Cat. No. 11972103122)
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Serum 25 OH vitamin D
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Radioimmunoassay, DiaSorin RIA kit (Cat. No. 68100E)
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Anthropmetric and clinical assessments:
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Blood pressure
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Omron HEM-907 Digital Automatic Blood Pressure Monitor
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Temperature
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Braun ThermoScan Pro 3000 aural thermometer
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Height, weight, waist and hip circumference
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ISAK anthropometry methods – ISAK level one accredited anthropometrist, Tanita electronic scales, stadiometer, Lufkin tape
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Questionnaires:
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Dietary assessment
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4-day food diary. Completed by subjects. Analysed using Foodworks 2007 (Xyris Software). Additional recipes and food lists provided by Indian dietitian
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Medical and family history
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Questionnaire administered following blood tests
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Demographics
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