Table 1 Outcome measures and testing methods used for phase one.
Measure | Method |
|---|---|
Blood analysis | All analysed by LabPlus, Auckland City Hospital, Auckland |
Fasting plasma insulin | Micro-particle enzyme immunoassay technology (MEIA), Abbott Diagnostics |
Fasting plasma glucose | An enzymatic colourimetric assay; Roche Glucose reagent kit (Cat. No. 1876899) |
Total cholesterol | Enzymatic colourimetric method (Roeschlau and Allain). Roche Cholesterol reagent kit (Cat. No. 1491458) |
Triglycerides | Enzymatic conversion. Roche Triglycerides reagent kit (Cat. No. 1727711) |
HDL-C | Homogenous enzymatic colourimetric assay. Roche HDL-Cholesterol Plus reagent kit (Cat. No. 04713214) |
LDL-C | Calculated |
Serum calcium | Colourimetric assay. Roche Calcium reagent kit (Cat. No. 1730240) |
Serum albumin | Colourimetric assay. Roche Albumin Plus reagent kit (Cat. No. 1970909) |
Serum high sensitivity CRP | Particle enhanced immunoturbidimetric assay. Roche CRPLX reagent kit (Cat. No. 03002039) |
Serum parathyroid hormone | "ECLIA" electrochemiluminescence immunoassay. PTH Reagent Pack (Cat. No. 11972103122) |
Serum 25 OH vitamin D | Radioimmunoassay, DiaSorin RIA kit (Cat. No. 68100E) |
Anthropmetric and clinical assessments: | |
Blood pressure | Omron HEM-907 Digital Automatic Blood Pressure Monitor |
Temperature | Braun ThermoScan Pro 3000 aural thermometer |
Height, weight, waist and hip circumference | ISAK anthropometry methods – ISAK level one accredited anthropometrist, Tanita electronic scales, stadiometer, Lufkin tape |
Questionnaires: | |
Dietary assessment | 4-day food diary. Completed by subjects. Analysed using Foodworks 2007 (Xyris Software). Additional recipes and food lists provided by Indian dietitian |
Medical and family history | Questionnaire administered following blood tests |
Demographics |