Follow-up in healthy schoolchildren and in adolescents with DOWN syndrome: psycho-environmental and genetic determinants of physical activity and its impact on fitness, cardiovascular diseases, inflammatory biomarkers and mental health; the UP&DOWN Study

Table 1 Haematological, biochemical and cardiometabolic variables

Haematological profile	Analytical techniques
Red blood cell, white blood cell, neutrophil, lymphocyte, monocyte, basophil, eosinophil, platelet counts, leukocyte profile, haemoglobin concentrations, haematocrit and haematic indices.	Automatic cell counter
Biochemical variables	Analytical techniques
Triglycerides, total cholesterol (c), HDL-c, LDL-c, glucose, and total proteins	Colorimetric assay (AU2700 Olympus analyser)
Cardio-metabolic variables and inflammatory	Analytical techniques
Visfatine	Enzyme-linked immunoSobent assay (Human visfatin Elisa kit; Cusabio Biotech)
TNF-α, IL-6, adiponectin, insulin, leptin	Immunoassay (xMAP Techonology) using a kit (5 + 1) plex: 171B5006M Bio-Plex Human IL-6 set; 171B5026M Bio-Plex Human TNF-α set; 171D50001 Bio-Plex Human Cytokine Stds; 171-A7003M Bio-plex Pro Human Diabetes Adiponectin Assay; YB0000002Y Bio-Plex Human Diabetes 3-Plex Assay
Cortisol	Enzyme-Linked ImmunoSorbent Assay (ARBOR ASSAYS kit)
C3 and C4 complement factors, and CRP	Turbidimetry (AU2700 Olympus analyser)
Fibrinogen	Clauss method
Galectin-3	Enzyme-Linked ImmunoSorbent Assay (Omnikine ^TM Human Galectin- 3 Elisa Kit, assay biotech)

HDL-c: high density lipoprotein cholesterol; LDL-c: low density lipoprotein cholesterol; TNF-α: tumor necrosis factor alpha; IL-6: inteleukin 6; C3: complement factor 3; C4: complement factor 4; CRP: complement factor reactive protein.

ISSN: 1471-2458