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Table 1 Haematological, biochemical and cardiometabolic variables

From: Follow-up in healthy schoolchildren and in adolescents with DOWN syndrome: psycho-environmental and genetic determinants of physical activity and its impact on fitness, cardiovascular diseases, inflammatory biomarkers and mental health; the UP&DOWN Study

Haematological profile Analytical techniques
Red blood cell, white blood cell, neutrophil, lymphocyte, monocyte, basophil, eosinophil, platelet counts, leukocyte profile, haemoglobin concentrations, haematocrit and haematic indices. Automatic cell counter
Biochemical variables Analytical techniques
Triglycerides, total cholesterol (c), HDL-c, LDL-c, glucose, and total proteins Colorimetric assay (AU2700 Olympus analyser)
Cardio-metabolic variables and inflammatory Analytical techniques
Visfatine Enzyme-linked immunoSobent assay (Human visfatin Elisa kit; Cusabio Biotech)
TNF-α, IL-6, adiponectin, insulin, leptin Immunoassay (xMAP Techonology) using a kit (5 + 1) plex: 171B5006M Bio-Plex Human IL-6 set; 171B5026M Bio-Plex Human TNF-α set; 171D50001 Bio-Plex Human Cytokine Stds; 171-A7003M Bio-plex Pro Human Diabetes Adiponectin Assay; YB0000002Y Bio-Plex Human Diabetes 3-Plex Assay
Cortisol Enzyme-Linked ImmunoSorbent Assay (ARBOR ASSAYS kit)
C3 and C4 complement factors, and CRP Turbidimetry (AU2700 Olympus analyser)
Fibrinogen Clauss method
Galectin-3 Enzyme-Linked ImmunoSorbent Assay (Omnikine TM Human Galectin- 3 Elisa Kit, assay biotech)
  1. HDL-c: high density lipoprotein cholesterol; LDL-c: low density lipoprotein cholesterol; TNF-α: tumor necrosis factor alpha; IL-6: inteleukin 6; C3: complement factor 3; C4: complement factor 4; CRP: complement factor reactive protein.