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Table 10 Biomarkers in blood

From: A single-blinded, single-centre, controlled study in healthy adult smokers to identify the effects of a reduced toxicant prototype cigarette on biomarkers of exposure and of biological effect versus commercial cigarettes

Biomarker Collection tube volume and type Matrix
2-cyanoethylvaline 2 × 4.9 mL EDTA Erythrocytes
4-ABP Hb adduct 2 × 4.9 mL EDTA Erythrocytes
IL-6, IL-8, MCP-1, TNF-a, VEGF, MMP-1, MMP-9, TIMP-1, ox LDL, superoxide dismutase activity, glutathione peroxidase activity, glutathione reductase activity, catalase activity, Hb (lysate) 1 × 9 mL EDTA Plasma, lysate
Total antioxidant capacity 1 × 4.7 mL serum Serum
Ascorbic acid, dehydroascorbic acid 1 × 7.5 mL EDTA Plasma
Neutrophil elastase, fibrinogen 1 × 5 mL citrate Plasma
Hb (lysate), malondialdehyde 1 × 7.5 mL li-heparin Lysate, cell pellet
sICAM-1 1 × 4 mL EDTA Plasma
hsCRP (males) 1 × 8.5 mL serum Serum
Lipid Profile 1 × 8.5 mL serum Serum
LTB4 1 × 4 mL li-heparin Plasma
White blood cells, neutrophils, monocytes, haemoglobin 1 × 2.7 mL EDTA Plasma
Gene expression 2.5 mL × 2 Paxgene Plasma
  1. 4-ABP Hb adduct, 4-aminobiphenyl haemoglobin adduct. IL-6, interleukin-6. IL-8, interleukin-8.
  2. MCP-1, Monocyte Chemoattractant Protein 1. TNF-a, Tumor necrosis factor alpha.
  3. VEGF, Vascular Endothelial Growth Factor. MMP, Matrix Metalloproteinase.
  4. TIMP-1, Tissue inhibitor of metalloproteinases. ox LDL, oxidised low density lipoprotein.
  5. Hb, Haemoglobin. sICAM-1, soluble intercellular adhesion molecule. hsCRP, high sensitivity C-reactive protein.
  6. LTB4, Leukotriene B4. EDTA, Ethylenediaminetetraacetic acid.