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Figure 2 | BMC Public Health

Figure 2

From: A local outbreak of dengue caused by an imported case in Dongguan China

Figure 2

Molecular Phylogenetic analysis of dengue virus isolates from this study compared with the other established dengue virus based on the alignment of E-protein gene. Phylogenetic analysis were conducted in MEGA5. The evolutionary history was inferred by using the Maximum Likelihood method based on the General Time Reversible model. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the dengue virus isolates analyzed. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The percentage of replicate trees in which the associated dengue virus isolates clustered together in the bootstrap test (1000 replicates) are shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically as follows. When the number of common sites was < 100 or less than one fourth of the total number of sites, the maximum parsimony method was used; otherwise BIONJ method with MCL distance matrix was used. The analysis involved 141 nucleotide sequences. There were a total of 1427 positions in the final dataset. The cDNA of DG2010, D10029-DG and D10030-DG in E-protein region shared 100% identity with each other. The molecular phylogeny analysis result indicated that the D10030-DG isolate has a nearest relationship with DENV1/D10167-GZ/2010 (GenBank: JN029814.1), DENV1/D10102-SZ/2010 (GenBank: JN029813.1), and DENV1/D10007-DG/2010(GenBank: JN029807) (Figure 2), which were isolated in Guangdong in 2010, and didn't cause local outbreak. This cluster is statistically supported (bootstrap value = 96%) and is within a clade contain the sequence DENV1/SG/(EHI)DED142808/2008, isolated in Singapore in 2008 also statistically supported (bootstrap value = 85%)

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