From: CARRS Surveillance study: design and methods to assess burdens from multiple perspectives
Clinical parameter | Laboratory parameter | Methods used for analysis | ||
---|---|---|---|---|
Chennai | Delhi | Karachi | ||
Diabetes | Fasting plasma glucose | Hexokinase/Kinetic | Hexokinase/Kinetic | Glucose Oxidase / End Point |
Glycated haemoglobin (HbA1c) | High performance liquid chromatography (HPLC) | HPLC | HPLC | |
Dyslipidemia | Total cholesterol | Cholesterol Oxidase Peroxidase (CHOD-POD) end point | CHOD-POD end point | Enzymatic Colorimetric method (CHOD-PAP) |
High density lipoprotein cholesterol | Direct | Direct | Direct | |
Low density lipoprotein cholesterol | Friedwald Formula | Friedwald Formula | Friedwald Formula | |
Very low density lipoprotein cholesterol | Calculation | Calculation | Calculation | |
Triglycerides | Enzymatic methods (GPO-PAP end point) | Enzymatic methods (GPO-PAP end point) | Enzymatic methods (GPO-PAP end point) | |
Apolipoprotein A, Apolipoprotein B | Immuno-turbidimetric | Immuno-turbidimetric | Will not be done | |
Kidney disease | Serum urea | Urease Glutamate Dehydrogenase (GLDH) / Kinetic | Urease GLDH/ Kinetic | Blood Urea Nitrogen (BUN): Enzymatic conductivity rate method |
Serum creatinine | Jaffe Kinetic | Jaffe Kinetic | Modified Jaffe’s Method | |
Microalbuminuria | Immuno-turbidimetry | Immuno-turbidimetry | Rate nephelometry | |
Tobacco exposure | Salivary cotinine | Elisa kit | Elisa kit | Elisa kit |