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Table 3 Biological samples and their methods of analysis

From: CARRS Surveillance study: design and methods to assess burdens from multiple perspectives

Clinical parameter Laboratory parameter Methods used for analysis
Chennai Delhi Karachi
Diabetes Fasting plasma glucose Hexokinase/Kinetic Hexokinase/Kinetic Glucose Oxidase / End Point
Glycated haemoglobin (HbA1c) High performance liquid chromatography (HPLC) HPLC HPLC
Dyslipidemia Total cholesterol Cholesterol Oxidase Peroxidase (CHOD-POD) end point CHOD-POD end point Enzymatic Colorimetric method (CHOD-PAP)
High density lipoprotein cholesterol Direct Direct Direct
Low density lipoprotein cholesterol Friedwald Formula Friedwald Formula Friedwald Formula
Very low density lipoprotein cholesterol Calculation Calculation Calculation
Triglycerides Enzymatic methods (GPO-PAP end point) Enzymatic methods (GPO-PAP end point) Enzymatic methods (GPO-PAP end point)
Apolipoprotein A, Apolipoprotein B Immuno-turbidimetric Immuno-turbidimetric Will not be done
Kidney disease Serum urea Urease Glutamate Dehydrogenase (GLDH) / Kinetic Urease GLDH/ Kinetic Blood Urea Nitrogen (BUN): Enzymatic conductivity rate method
Serum creatinine Jaffe Kinetic Jaffe Kinetic Modified Jaffe’s Method
Microalbuminuria Immuno-turbidimetry Immuno-turbidimetry Rate nephelometry
Tobacco exposure Salivary cotinine Elisa kit Elisa kit Elisa kit