Table 5 Biobank blood sample collection and parameters of direct analysis
From: The LIFE child study: a life course approach to disease and health
Participant age | Maximum volume of stored aliquots | Direct analysis |
|---|---|---|
Birth | 24 x 0.3 ml (EDTA, SG) | Not performed |
3 Month | 4 x 0.3 ml (EDTA, SG) | Electrolytes, bilirubin, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters, vitamins, NT-pro-BNP, growth hormones |
6 Month | 6 x 0.3 ml (EDTA, SG) | Electrolytes, bilirubin, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters, vitamins, allergy testing, troponin T, NT- pro-BNP, growth hormones |
1 – 3 years | 8 x 0.3 ml (EDTA, SG) | Electrolytes, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters,vitamins, allergy testing, troponin T, NT- pro-BNP, growth hormones, liver function tests, renal function tests, transferrin, sex steroids |
3 ml (Blood RNA tube) | ||
3 – 6 years | 10 x 0.3 ml (EDTA, SG) | Electrolytes, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters,vitamins, allergy testing, troponin T, NT- pro-BNP, growth hormones, liver function tests, renal function tests, transferrin, ferritin, sex steroids |
3 ml (Blood RNA tube) | ||
4 ml (Cell preparation tube) | ||
6 – 8 years | 12 x 0.3 ml (EDTA, SG) | Electrolytes, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters,vitamins, allergy testing, troponin T, NT- pro-BNP, growth hormones, liver function tests, renal function tests, transferrin, glucose, insulin, sex steroids |
3 ml (Blood RNA tube) | ||
4 ml (Cell preparation tube) | ||
LIFE Child OBESITY cohort and controls : | ||
+ Oral glucose tolerance test, proinsulin, c-peptide, uric acid | ||
8 – 14 years | 20 x 0.3 ml (EDTA, SG) | See 6 – 8 years |
3 ml (Blood RNA tube) | ||
8 ml (Cell preparation tube) | ||
> 14 years | 26 x 0.3 ml (EDTA, SG) | See 6 – 8 years |
3 ml (Blood RNA tube) | ||
8 ml (Cell preparation tube) | ||
Parents | 24 x 0.3 ml (EDTA, SG) | Not performed |
3 ml (Blood RNA tube) | ||
8 ml (Cell preparation tube) | ||
Pregnant Women (24 th - 26 th week of gestation, 34 th - 36 th week of gestation and visits postpartum) | 30 x 0.3 ml (EDTA, SG) | Electrolytes, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters,vitamins, allergy testing, growth hormones, liver function tests, renal function tests, transferrin,glucose, insulin, sex steroids, oral glucose tolerance test |
3 ml (Blood RNA tube) | ||
8 ml (Cell preparation tube) | ||
34th -36th week of gestation: no oral glucose tolerance test | ||
Visits postpartum: no renal function tests |