Table 3 Haematological and Immunological profiles.
Haematological profile | Analytical Techniques |
|---|---|
Red blood cell counts and indices White blood cell counts and differential | Automatic cell counter (Beckman coulter) |
Cell-mediated immunity | Analytical Techniques |
Lymphocyte subsets:CD3, CD4, CD8, CD16/56, CD45RA, CD45RO | Flow Cytometry [55] |
Cellular immune function: Cytokines in serum | Analytical Techniques |
IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF-α | Immunoassay (x Map technology Linco kits) (56). Human High Sensitivity Kit. |
TGF-β | Enzyme Linked Immunoabsorbent Assay (ELISA) |
Humoral immunity | Analytical Techniques |
Lymphocyte subset CD19 | Flow Cytometry [55] |
Humoral function: IgG, IgA, IgM, IgE | Nephelometry |
Innate immunity | Analytical Techniques |
C3, C4 and C-reactive protein. | Nephelometry |
Adhesion molecules | Analytical Techniques |
L-selectin, ICAM-1, VCAM-1 | Immunoassay (x Map technology Linco kits) Cardiovascular CVD1 Kit |
E-selectin | Enzyme Linked Immunoabsorbent Assay (ELISA) |
Adipokines | Analytical Techniques |
Adiponectin, leptin | Immunoassay (x Map technology Linco kits) Cardiovascular CVD1 Kit |