Airborne rhinovirus detection and effect of ultraviolet irradiation on detection by a semi-nested RT-PCR assay

BMC Public Health

Table 1 Detection of Experimental Rhinovirus 16 Aerosols by Semi-nested RT-PCR of Filter Samples

	Nebulizing Solution
Run Number	TCID₅₀ per ml	Total ml Nebulized	Fractional Sampling Time per Filter^a	Yield^b	Predicted TCID₅₀ per Filter^c	Positive Filters/Total
VA Run 12	16.1	15.2	0.136	0.041	1.4	3/3
VA Run 17	10.1	24.2	0.085	0.103	2.1	3/3
VA Run 13	6.5	15.0	0.142	0.093	1.3	2/3
VA Run 14	3.3	16.7	0.119	0.072	0.5	0/3

^aSampling time of each filter (10 minutes) divided by total run time. ^bFluorescence yield (observed/expected) for sampling run. ^cPredicted = TCID₅₀ per ml * Total ml nebulized * Fractional sampling time per filter * Yield.

ISSN: 1471-2458