Women's knowledge and attitudes regarding alcohol consumption in pregnancy: a national survey
© Peadon et al; licensee BioMed Central Ltd. 2010
Received: 5 March 2010
Accepted: 23 August 2010
Published: 23 August 2010
Alcohol exposure in pregnancy is a common and modifiable risk factor for poor pregnancy and child outcomes. Alcohol exposure in pregnancy can cause a range of physical and neurodevelopmental problems in the child including the Fetal Alcohol Spectrum Disorders (FASD). In order to improve prevention strategies, we sought to describe the knowledge and attitudes of women of childbearing age regarding alcohol consumption during pregnancy and its effects on the fetus.
We conducted a national cross-sectional survey via computer assisted telephone interview of 1103 Australian women aged 18 to 45 years. Participants were randomly selected from the Electronic White Pages. Pregnant women were not eligible to participate. Quotas were set for age groups and a minimum of 100 participants per state to ensure a national sample reflecting the population. The questionnaire was based on a Health Canada survey with additional questions constructed by the investigators. Descriptive statistics were calculated and logistic regression analyses were used to assess associations with participants' knowledge and attitudes.
Of women surveyed, 61.5% had heard about effects of alcohol on the fetus and 55.3% had heard of Fetal Alcohol Syndrome. Although 92.7% agreed alcohol can affect the unborn child, 16.2% did not agree that the disabilities could be lifelong. Most women agreed that pregnant women should not drink alcohol (80.2%) and 79.2% reported having negative feelings towards pregnant women drinking alcohol. Women with higher education levels were more likely to know the effects of alcohol consumption in pregnancy (adjusted OR 5.62; 95% CI 3.20 to 9.87) but education level and knowledge were not associated with attitude.
There was a disjunction between knowledge and attitudes towards alcohol consumption in pregnancy. These findings will assist in developing effective health promotion campaigns to reduce fetal alcohol exposure and subsequent fetal damage.
Alcohol exposure is one of the few modifiable risk factors for poor pregnancy outcomes. Fetal Alcohol Syndrome (FAS) was formally described only thirty-five years ago but the effects of alcohol consumption in pregnancy on the unborn child have been recognised for hundreds of years. Alcohol consumption in pregnancy has been associated with miscarriage, premature birth, stillbirth, low birth weight and diagnoses that are encompassed by the umbrella term of the Fetal Alcohol Spectrum Disorders (FASD). Children with FAS have characteristic facial features (small palpebral fissures, smooth philtrum and thin vermillion border of the upper lip), prenatal and/or postnatal growth retardation, and central nervous system structural and/or functional abnormalities. Partial FAS, Alcohol Related Neurodevelopmental Disorder (ARND) and Alcohol Related Birth Defects (ARBD) are other diagnoses covered by FASD.
Alcohol consumption is common in many cultures and consumption in young women has increased over the past thirty years, with an increasingly frequent pattern of risky drinking and increased risk of unintentional alcohol consumption during pregnancy[7–9]. Up to 50% of pregnancies are unplanned and there is an association between binge drinking and unplanned conception[8, 9]. Consequently, there is potentially a high rate of fetal exposure to alcohol.
We have estimates of alcohol consumption in pregnancy, however we have little contemporary data about women's knowledge and attitudes regarding alcohol consumption in pregnancy and how these are associated with alcohol consumption in pregnancy. In order to design effective health promotion strategies to reduce alcohol consumption in pregnancy, we need to understand potential influences on women's behaviour. Our aims in this study were to ascertain women's knowledge and attitudes regarding alcohol consumption in pregnancy and its effects on the unborn child and to identify maternal factors associated with knowledge and attitudes.
Participants and Sampling
Random sampling was achieved by computer-generated national sampling from the Electronic White Pages. Women aged 18 to 45 years inclusive were eligible to participate in the survey. Pregnant women were deemed ineligible to participate to comply with the Ethics Committee's recommendation that the topic might generate anxiety. Standard questions regarding age, gender and pregnancy status were used to screen participants' eligibility. The survey was administered in English. To ensure that the survey population was a representative national sample, we stratified the sample by age group (18 to 29 years and 30 to 45 years) and state of residency. We set quotas to reflect the age distribution of Australian women in the 18 to 45 year age group in 2003, and set a minimum of 100 participants from each state. The survey was administered as a computer assisted telephone interview by the Survey Research Centre, School of Population Health, University of Western Australia in August and September, 2006.
Our "Alcohol and Pregnancy Questionnaire" was based on the Health Canada survey "Alcohol Use During Pregnancy and Awareness of Fetal Alcohol Syndrome" but the language was modified for the Australian context, with additional questions on women's attitudes to alcohol consumption in pregnancy (relating to cognitions and feelings). Decisions about changes to wording of the Health Canada survey questions and the construction of additional questions were made by investigator consensus. A pilot study of 20 randomly selected women was conducted in August 2006. Feedback from the pilot sample was reviewed by the investigators and was used to inform minor changes made to the questionnaire. The changes were clarification of instructions to the survey administrators and changes to recording and coding of responses for some questions. We collected demographic data (age, ethnicity, parity, postcode, educational level, employment, marital status) and data on women's knowledge and attitudes regarding alcohol consumption in pregnancy; and their knowledge of the effects of alcohol on the fetus.
A sample size of n = 1100 provided estimates within 2% for variables with a prevalence of 40 to 50%. Data were analysed using SPSS (version 15.0, SPSS Chicago, IL, USA). Descriptive statistics of responses to survey questions were calculated. Associations between demographic characteristics and the survey outcomes, knowledge and attitudes regarding alcohol consumption in pregnancy were investigated, as well as the association between knowledge and attitude. Forward stepwise logistic regression analysis was conducted to analyse which factors were associated with knowledge and attitudes. Odds ratios and 95% confidence intervals (CI) were calculated. These analyses included: age, educational attainment, pregnancy history, smoking status, alcohol consumption status, relationship status, country of birth (Australia versus overseas), and employment status. Dichotomous variables were created for age (18 to 29 years versus 30 to 45 years), pregnancy history (previously given birth versus nulliparous), smoking status (current smoker versus non or ex-smoker), alcohol consumption status (drank alcohol in the last 12 months versus no alcohol in the past 12 months), relationship status (de facto partner or married versus single), and employment status (employed versus not employed). Educational attainment responses were categorized as completed less than Year 12, completed Year 12, completed a post-school qualification lower than a bachelor degree and completed a bachelor degree or higher. Knowledge responses were obtained from yes/no questions or agreement on a 5-point Likert scale. The responses on the Likert scale were dichotomised (agree versus not agree). Participants' attitudes to the scenario of seeing a pregnant woman drinking were dichotomised to negative versus neutral or positive. A priori, age, educational attainment and pregnancy history were considered potential confounders and all associations were adjusted for these factors.
The study protocol was approved by the Ethics Committees of the Women's and Children's Health Service, Perth, Western Australia, The University of Sydney and the Western Australian Aboriginal Health Information and Ethics Committee.
Characteristics of survey respondents (n = 1103)
Age, years, mean, (standard deviation)
Given birth to a child
Years since last child born, median (range)
(<1 to 23)
Planning pregnancy in the next 2 years
Planning pregnancy sometime in the future (n = 873)
Married (or de facto/life partner)
Aboriginal or Torres Strait Islander origin
Country of Birth
Years in Australia if born outside Australia, median (range)
(<1 to 44)
Current employment status (n = 1102)
Unemployed or retired or on a pension
Highest educational attainment
Year 11 or below
Post-school qualification below bachelor degree
Drank alcohol in the past 12 months
Knowledge about alcohol and pregnancy
Knowledge of the effects on the unborn child of alcohol consumption during pregnancy
Have ever heard of any effects on pregnancy or the unborn child which are caused by drinking alcohol during pregnancy (n = 1103)
Nominated effects of alcohol on pregnancy or the unborn child (n = 678)*
Fetal Alcohol Syndrome
Low birth weight
Baby addicted/experiences withdrawal
Causes birth defects/deformities
Baby born with alcohol in its system
Attention Deficit Disorder
Alcohol Related Neurodevelopmental Disorder
Alcohol Related Birth Defects
Fetal Alcohol Spectrum Disorders
Fetal Alcohol Effects
Have ever heard of (n = 1103)
Fetal Alcohol Syndrome
Alcohol Related Birth Defects
Alcohol Related Neurodevelopmental Disorder
Fetal Alcohol Spectrum Disorders
The respondents were asked if they had heard of specific disorders associated with alcohol exposure in pregnancy. The proportion of respondents who said "yes" varied from 55.3% for Fetal Alcohol Syndrome to 8.5% for Fetal Alcohol Spectrum Disorder (Table 2). Respondents who had heard of a disorder were asked to describe the characteristics of the disorder. Knowledge of the characteristics of these conditions was poor. "Don't know" was the most or second most common response when a respondent who had heard of a condition was asked to describe the disorder, ranging from 55.3% for FASD to 21.8% for FAS. Incorrect or non-specific responses such as "Effects of alcohol on fetus" were also common.
Attitudes about alcohol consumption in pregnancy
Attitudes about alcohol consumption in pregnancy were assessed with a series of questions relating to cognitions and feelings. Respondents were asked to rate their agreement with statements about alcohol and pregnancy, some including an element of approval/disapproval, on a five-point Likert scale ranging from strongly agree to strongly disagree. Most respondents (80.2%) agreed that pregnant women should not drink alcohol; 37.2% agreed and 43.1% strongly agreed with this statement. Most (92.7%) agreed that drinking alcohol during pregnancy can affect the unborn child and that the more alcohol a woman drinks, the more likely the unborn child will be harmed (96.6%); however 16.2% did not agree that drinking alcohol during pregnancy can lead to lifelong disabilities in a child. Only 67.5% of respondents agreed that women are aware of the effects that drinking alcohol during pregnancy can have on the unborn child. More than three quarters of respondents (78.8%) agreed that members of the community are concerned about women drinking alcohol during pregnancy. Most women (91.9%) agreed that pregnant women should drink less than seven standard alcohol drinks each week, and only a minority agreed that it is ok for pregnant women to drink three or four standard alcohol drinks on one day (2.1%) and that it is ok for pregnant women to become intoxicated (0.8%).
Factors associated with knowledge of the effects on the unborn child of alcohol consumption in pregnancy (Table 3)
Factors associated with knowledge of the specific effects on the child of alcohol exposure in pregnancy
Know one or more of the diagnoses resulting from alcohol exposure in pregnancy
Know that alcohol causes growth problems
Agree that drinking alcohol during pregnancy can lead to lifelong disabilities in a child
Agree that pregnant women should not drink alcohol
Report negative feeling to women drinking alcohol in pregnancy
Adjusted OR† (95% CI)
Highest education level
Completed Year 12
1.87* (1.01 to 3.46)
0.98 (0.56 to 1.70)
1.13 (0.68 to 1.89)
1.08 (0.64 to 1.81)
0.80 (0.48 to 1.34)
Post school below bachelor degree
1.48 (0.83 to 2.65)
1.52 (0.95 to 2.44)
1.32 (0.85 to 2.07)
0.88 (0.56 to 1.38)
0.89 (0.57 to 1.40)
Bachelor degree or higher
5.62** (3.20 to 9.87)
2.04* (1.26 to 3.31)
2.15* (1.28 to 3.61)
0.87 (0.54 to 1.40)
0.75 (1.47 to 1.21)
Alcohol can cause lifelong disabilities
4.59** (3.22 to 6.54)
3.67** (2.56 to 5.29)
Not given birth to a child
Given birth to a child
1.13 (0.77 to 1.67)
1.24 (0.84 to 1.83)
0.64 (0.40 to 1.01)
0.59* (0.39 to 0.88)
0.39** (0.26 to 0.60)
0.48** (0.34 to 0.69)
0.78 (0.53 to 1.14)
18 to 29 years
30 to 45 years
0.72 (0.49 to 1.06)
1.18 (0.81 to 1.72)
0.67 (0.43 to 1.03)
1.01 (0.69 to 1.48)
1.14 (0.77 to 1.68)
Knowledge was related to education level. Women who had a bachelor degree or higher were more likely than those who had left school before the end of year 12 to know that alcohol consumption in pregnancy can cause one or more of the diagnoses resulting from alcohol exposure in pregnancy (OR 5.60 95% CI 3.22 to 9.74; p < 0.001), and to know that drinking alcohol during pregnancy can lead to lifelong disabilities in a child (OR 2.52 95% CI 1.52 to 4.19; p < 0.001). Women who had previously given birth were less likely to know that the disabilities can be lifelong than nulliparous women (OR 0.48 95% CI 0.33 to 0.69; p < 0.001). Similarly, women aged 30-45 years were less likely than younger women to know that alcohol can cause growth problems or that it can cause lifelong disabilities. After adjusting for potential confounders, level of education remained the only statistically significant predictor of women's knowledge.
Factors associated with attitudes to alcohol consumption in pregnancy (Table 3)
Agreeing that alcohol causes lifelong disabilities, planning a future pregnancy, age, pregnancy history and smoking status were associated with attitudes to alcohol consumption during pregnancy. After adjusting for age, level of education and pregnancy history, women who agreed that alcohol consumption in pregnancy can cause lifelong disabilities were more likely than women without this knowledge to agree that pregnant women should not drink (adjusted OR 4.59 95% CI 3.22 to 6.54; p < 0.001) and to have a negative attitude towards alcohol consumption in pregnancy (adjusted OR 3.67 95% CI 2.56 to 5.29; p < 0.001). This was the only significant association between knowledge and attitude. Women who had given birth previously or were smokers were more likely to have a tolerant attitude towards alcohol consumption in pregnancy. Education level was not associated with attitudes to alcohol consumption in pregnancy.
This survey provides contemporary national data on Australian women's knowledge and attitudes about alcohol consumption in pregnancy and its effects on the fetus. While there was an encouraging baseline level of knowledge regarding alcohol use in pregnancy and its effects on the fetus, it is of concern that one in three women of child bearing age said they did not know any adverse effects of alcohol consumption in pregnancy. Many women who said they were aware of adverse effects could not name any specific effects. Most women agreed that pregnant women should not drink alcohol and reported a negative attitude towards alcohol consumption in pregnancy, although one fifth had a neutral or positive attitude towards alcohol consumption in pregnancy.
Comparison of results of this survey with those from the 2006 Canadian survey showed some differences in knowledge. Australian women were less likely than Canadian women (83.8% vs 96%) to agree that alcohol consumption in pregnancy can lead to life long disabilities. Fewer Australian than Canadian women had heard of the terms FAS or FASD (56.4% vs 88%). Australian women were also more likely to report they did not know any specific characteristics of these disorders (25.7% vs 18%). This is the third iteration of the Canadian survey and there has been an increase in knowledge since the first survey in 1999, which may be the outcome of several FASD awareness campaigns conducted in Canada.
Direct comparison to other studies is hampered by the use of different questions; however, women's knowledge in this study was generally similar to that in other studies. In a French study, 83% of respondents knew that alcohol in pregnancy could be harmful to the baby. In a Danish study, 85% of respondents knew that alcohol is possibly harmful to the fetus. However, a much smaller proportion of Danish women stated that pregnant women should not drink alcohol than in this study (80.2% vs 24%). This may be due to differences in methodology, as the Danish women gave a spontaneous answer to an open-ended question, whereas the women in this study were asked to agree or disagree with a statement.
Women with higher levels of education had more knowledge about the specific effects of alcohol consumption in pregnancy, which has been found in previous studies. However, women's attitudes towards alcohol consumption in pregnancy were not associated with level of education (the Canadian survey did not report women's attitudes). Agreeing that alcohol in pregnancy can cause lifelong disabilities in the child was the only knowledge associated with a negative attitude.
Knowledge, experiences, social influence, habits, self confidence, motivation, attitudes and possibility for change have all been identified as determinants of health behaviour. One common theory is that knowledge is required to influence attitude which then leads to changes in health behaviour. However, other models suggest that there is a more complex pathway leading to changes in health behaviour, e.g. experiences and social influence may cause changes in self-confidence and attitude which lead to changes in health behaviour. Other studies have also found a disjunction between knowledge and attitudes, which may be mediated by risk perception[19, 21]. Risk perception, which is strongly influenced by individual experience, is an important predictor of alcohol consumption during pregnancy[21, 22]. Differences in risk perception may explain the differences in attitudes between nulliparous and multiparous women. Women who have had a previously health pregnancy report a lower perceived risk from alcohol consumption than women experiencing their first pregnancy.
Traditional public health approaches have concentrated on increasing public knowledge through awareness campaigns[17, 21]. However, it is clear that approaches to the prevention of FASD must encompass more than education. Awareness campaigns in isolation are doomed to failure as they do not address attitudes, social influence or experience. The data from this study can be used to inform development of effective health promotion campaigns and identify groups who require targeted strategies. These findings suggest we need a combination of strategies at community and society levels to inform Australian women's knowledge and influence attitudes about alcohol and pregnancy. At the community level, strategies could include education in schools and social marketing campaigns. Prevention of tobacco smoking campaigns may provide successful models which can be adapted for this issue. At the society level, governments should disseminate clear guidelines about alcohol consumption in pregnancy to health professionals and provide funding for public health campaigns. Other government strategies for raising awareness include legislation for compulsory labelling of alcoholic beverages.
This is the first national Australian study reporting women's knowledge and attitudes towards alcohol consumption in pregnancy and the first study to explore the relationship between knowledge and attitudes. The study has several strengths: the response rate was high (nearly 70% of eligible women contacted agreed to participate) and the study population was similar to the Australian population of women aged 18 to 45 years, although with a higher level of education. As knowledge of the effects of alcohol consumption in pregnancy is associated with level of education, our estimate of the level of knowledge in the general population may be inflated. A limitation of the study is that it was not specifically designed to capture knowledge and attitudes of Indigenous women, who comprised 1.9% of our sample, slightly lower than the proportion in the general population (2.4%). The knowledge and attitudes regarding alcohol and pregnancy may be different in Indigenous women compared to non-indigenous women and a separate study of Indigenous women's knowledge and attitudes is warranted. Also, teenagers under the age of 18 years were omitted from this study. There were 11, 977 births to Australian teenagers in 2006. As there has been an increase in the proportion of teenagers drinking at harmful levels since 1990, contemporary research into their knowledge and attitudes towards alcohol consumption in pregnancy is needed.
In this study, we found that Australian women of childbearing age have poor knowledge of the specific effects of alcohol in pregnancy on the unborn child and one in five women have a neutral or positive attitude towards alcohol consumption in pregnancy. We also found a disjunction between women's knowledge and attitudes. Interventions based on a theoretical model of health behaviour and which address past experience, social influence, risk perception and identified gaps in knowledge and misconceptions may be more successful than the traditional educational approaches. Public health campaigns, national policy and guidelines must all be underpinned by a regularly reviewed scientific evidence base of the influences on women's alcohol behaviour in pregnancy and effective prevention strategies.
We thank the women who participated in the computer assisted telephone survey. We acknowledge the support of the Alcohol and Pregnancy Steering Committee. We also acknowledge the members of the Aboriginal Community Reference Group (Rhonda Cox, Lyn Dimer, Michael Doyle, Paula Edgill, Gloria Khan, Michael Wright) and members of the Community and Consumer Reference Group (Pip Brennan, Kiely O'Flaherty, Julie Whitlock). We acknowledge the support of the Health Promotion Foundation of Western Australia (Healthway Starter Grant 16163) and the National Health and Medical Research Council (Enabling Grant 402784, Program Grant 353514, Research Fellowship CB 353625, Practitioner Fellowship EE 457084 and Postgraduate Scholarship EP 457230).
- Jones KL, Smith DW: Recognition of the fetal alcohol syndrome in early infancy. Lancet. 1973, 2: 999-1001. 10.1016/S0140-6736(73)91092-1.View ArticleGoogle Scholar
- Lemoine P: The history of ethanol fetopathies. Journal of FAS International. 2003, 1: E2-Google Scholar
- Maconochie N, Doyle P, Prior S, Simmons R: Risk factors for first trimester miscarriage--results from a UK-population-based case-control study. BJOG. 2007, 114 (2): 170-186. 10.1111/j.1471-0528.2006.01193.x.View ArticlePubMedGoogle Scholar
- Sokol RJ, Janisse JJ, Louis JM, Bailey BN, Ager J, Jacobson SW, Jacobson JL: Extreme prematurity: an alcohol-related birth effect. Alcohol Clin Exp Res. 2007, 31: 1031-1037. 10.1111/j.1530-0277.2007.00384.x.View ArticlePubMedGoogle Scholar
- Burd L, Roberts D, Olson M, Odendaal H: Ethanol and the placenta: A review. J Matern Fetal Neonatal Med. 2007, 20: 361-375. 10.1080/14767050701298365.View ArticlePubMedGoogle Scholar
- Centers for Disease Control and Prevention, National Center on Birth Defects and Developmental Disabilities, National Task Force on Fetal Alcohol Syndrome and Fetal Alcohol Effects: Fetal Alcohol Syndrome: guidelines for referral and diagnosis. 2004, Atlanta: Centers for Disease Control and PreventionGoogle Scholar
- Chikritzhs T, Catalano P, Stockwell T, Donath S, Ngo H, Young D, Matthews S: Australian Alcohol Indicators. 2003, Perth, Western Australia: National Drug Research InstituteGoogle Scholar
- Allard-Hendren R: Alcohol use and adolescent pregnancy. MCN Am J Matern Child Nurs. 2000, 25: 159-162. 10.1097/00005721-200005000-00010.View ArticlePubMedGoogle Scholar
- Flanigan B, McLean A, Hall C, Propp V: Alcohol use as a situational influence on young women's pregnancy risk-taking behaviors. Adolescence. 1990, 25: 205-214.PubMedGoogle Scholar
- Colvin L, Payne J, Parsons D, Kurinczuk JJ, Bower C: Alcohol consumption during pregnancy in nonindigenous west Australian women. Alcohol Clin Exp Res. 2007, 31: 276-284. 10.1111/j.1530-0277.2006.00303.x.View ArticlePubMedGoogle Scholar
- Australian Historical Population Statistics (cat. no. 3105.0.65.001). [http://www.ausstats.abs.gov.au/ausstats/subscriber.nsf/0/CA2568A90021A807CA256DB000025315/$File/3105065001_table19.xls]
- Awareness of the effects of alcohol use during pregnancy and Fetal Alcohol Syndrome. [http://www.phac-aspc.gc.ca/publicat/fas-saf-natsurv-2000/index-eng.php]
- Cat. No. 2068.0 - 2006 Census of Population and Housing Australia. [http://www.censusdata.abs.gov.au]
- Cat. No. 6278.0 - Education and Training Experience, Australia. [http://www.abs.gov.au/AUSSTATS/abs@.nsf/DetailsPage/6278.02005]
- Australian Institute of Health and Welfare: 2007 National Drug Strategy Household Survey: first results. Drug Statistics Series number 20. Cat. no. PHE 98. 2008, Canberra: AIHWGoogle Scholar
- Environics Research Group: Alcohol use during pregnancy and awareness of Fetal Alcohol Syndrome and Fetal Alcohol Spectrum Disorder. 2006, Toronto, CanadaGoogle Scholar
- Public Health Agency of Canada: What we have learned: key Canadian FASD awareness campaigns. 2006, Ottawa, Canada: Health CanadaGoogle Scholar
- Lelong N, Kaminski M, Chwalow J, Bean K, Subtil D: Attitudes and behavior of pregnant women and health professionals towards alcohol and tobacco consumption. Patient Educ Couns. 1995, 25: 39-49. 10.1016/0738-3991(94)00695-I.View ArticlePubMedGoogle Scholar
- Kesmodel U, Schioler KP: Drinking during pregnancy: attitudes and knowledge among pregnant Danish women, 1998. Alcohol Clin Exp Res. 2002, 26: 1553-1560.View ArticlePubMedGoogle Scholar
- Meillier LK, Lund AB, Kok G: Cues to action in the process of changing lifestyle. Patient Educ Couns. 1997, 30: 37-51. 10.1016/S0738-3991(96)00957-3.View ArticlePubMedGoogle Scholar
- Testa M, Reifman A: Individual differences in perceived riskiness of drinking in pregnancy: antecedents and consequences. J Stud Alcohol. 1996, 57: 360-367.View ArticlePubMedGoogle Scholar
- Raymond N, Beer C, Glazebrook C, Sayal K: Pregnant women's attitudes towards alcohol consumption. BMC Public Health. 2009, 9: 175-10.1186/1471-2458-9-175.View ArticlePubMedPubMed CentralGoogle Scholar
- Laws PJ, Hilder L: Australia's mothers and babies 2006. Perinatal statistics series no.22. Cat. no. PER 46. 2008, Sydney: AIHW National Perinatal Statistics UnitGoogle Scholar
- White V, Hayman J: Australian secondary school students' use of alcohol in 2005. 2006, Canberra: Australian Government Department of Health and AgeingGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2458/10/510/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.